Korean Journal of Acupuncture

Society for Meridian and Acupoint (경락경혈학회)
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Suppression of Inflammatory Macrophage Responese by Glycyrrhiza Uralensis Herbal Acupuncture Extract

감초 약침액이 대식세포주에서 항염증효과에 미치는 영향

  • Bak, Jong-Phil (The Clinical Trial Center for Bio-Industry Se-Myung University) ;
  • Son, Jeong-Hyun (The Clinical Trial Center for Bio-Industry Se-Myung University) ;
  • Kim, Yong-Min (The Clinical Trial Center for Bio-Industry Se-Myung University) ;
  • Lee, Eun-Yong (Dept. of Acupuncture & Moxibustion, College of Korean Medicine, Se-Myung University) ;
  • Leem, Kang-Hyun (Dept. of Herbology, College of Korean Medicine, Se-Myung University) ;
  • Kim, Ee-Hwa (Dept. of Meridian & Acupoint, College of Korean Medicine, The Clinical Trial Center for Bio-Industry Se-Myung University)
  • 박종필 (세명대학교 한방바이오산업 임상지원센터) ;
  • 손정현 (세명대학교 한방바이오산업 임상지원센터) ;
  • 김용민 (세명대학교 한방바이오산업 임상지원센터) ;
  • 이은용 (세명대학교 한의과대학 침구학교실) ;
  • 임강현 (세명대학교 한의과대학 본초학교실) ;
  • 김이화 (세명대학교 한의과대학 경락경혈학교실)
  • Received : 2011.11.08
  • Accepted : 2011.12.12
  • Published : 2011.12.27
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Abstract

목적 : 본 연구는 감초의 염증에 대한 효과를 연구하였다. 감초의 에탄올 그리고 물로 추출한 두 가지의 약침액을 이용하여 쥐의 대식세포에서 유래한 RAW264.7 세포에 대한 염증 억제효과를 확인하였다. 방법 : Inducible nitric oxide synthase(iNOS), cyclooxygenase-2(COX-2)를 포함한 염증성 단백질의 발현과 extracellular signal-regulated kinase 1/2(ERK1/2) 그리고 phosphorylated ERK1/2 의 발현을 Western blot 으로 확인하였고, PGE2의 발현은 ELISA 로 확인하였다. 결과 : RAW264.7 세포에 감초의 물 혹은 에탄올 추출 약침액을 투여한 결과 투여된 농도에 따라 LPS로 유도된 NO의 생성이 억제되었으며 iNOS, COX-2, 그리고 인산화 ERK1/2 의 발현도 감소되었다. 결론 : 본 실험 결과, 적작약의 물 그리고 에탄올 추출 약침액에 대하여 항염증성 효과가 있음을 확인하였다.

Keywords

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