Journal of Oral Medicine and Pain

Korean Academy of Orofacial Pain and Oral Medicine (대한안면통증구강내과학회)
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A Comparison Study on Animal Models for Osteoarthritis in Temporomandibular Joint

측두하악관절에서의 골관절염 유도 동물모델 비교연구

  • Yu, Sun-Nyoung (Department of Microbiology & Immunology, School of Medicine, Pusan National University) ;
  • Yi, Young-Chul (Department of Oral Medicine, School of Dentistry, Pusan National University) ;
  • Park, Hae-Ryoun (Department of Oral Pathology, School of Dentistry, Pusan National University) ;
  • Ryu, Mi-Heon (Department of Oral Pathology, School of Dentistry, Pusan National University) ;
  • Jeon, Hye-Mi (Department of Oral Medicine, School of Dentistry, Pusan National University) ;
  • Kim, Kwang-Youn (Department of Microbiology & Immunology, School of Medicine, Pusan National University) ;
  • Kim, Sang-Hun (Department of Microbiology & Immunology, School of Medicine, Pusan National University) ;
  • Ok, Soo-Min (Department of Oral Medicine, School of Dentistry, Pusan National University) ;
  • Ko, Myung-Yun (Department of Oral Medicine, School of Dentistry, Pusan National University) ;
  • Ahn, Yong-Woo (Department of Oral Medicine, School of Dentistry, Pusan National University) ;
  • Ahn, Soon-Cheol (Department of Microbiology & Immunology, School of Medicine, Pusan National University) ;
  • Jeong, Sung-Hee (Department of Oral Medicine, School of Dentistry, Pusan National University)
  • 유선녕 (부산대학교 의학전문대학원 미생물학 및 면역학 교실) ;
  • 이영철 (부산대학교 치의학전문대학원 구강내과학 교실) ;
  • 박혜련 (부산대학교 치의학전문대학원 구강병리학 교실) ;
  • 유미현 (부산대학교 치의학전문대학원 구강병리학 교실) ;
  • 전혜미 (부산대학교 치의학전문대학원 구강내과학 교실) ;
  • 김광연 (부산대학교 의학전문대학원 미생물학 및 면역학 교실) ;
  • 김상헌 (부산대학교 의학전문대학원 미생물학 및 면역학 교실) ;
  • 옥수민 (부산대학교 치의학전문대학원 구강내과학 교실) ;
  • 고명연 (부산대학교 치의학전문대학원 구강내과학 교실) ;
  • 안용우 (부산대학교 치의학전문대학원 구강내과학 교실) ;
  • 안순철 (부산대학교 의학전문대학원 미생물학 및 면역학 교실) ;
  • 정성희 (부산대학교 치의학전문대학원 구강내과학 교실)
  • Received : 2011.10.04
  • Accepted : 2011.11.16
  • Published : 2011.12.30
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Abstract

Osteoarthritis in patients with temporomandibular disorders(TMDs) induces pain, limitation of mouth opening, occlusal problems, and most commonly affects their life quality. Control method and progressive process of osteoarthritis are being extensively researched. The researchers focus on histologic changes, synovial changes, muscular and ligamental changes and observed reaction to pain. Therefore most of them developed the animal model for osteoarthritis in TMD patients. In this study, we applied several methods which induces osteoarthritis of temporomandibular joint(TMJ) in rats or mice. For locally induce osteoarthritis in TMJ, Monosodium iodoacetate(MIA) or interleukin-$1{\alpha}$(IL-$1{\alpha}$) were injected into TMJ joint space for 5 or 3 weeks. Other groups are chosen for osteoarthritis under systemic control including hormonal changes and aging. To observe cellular change, increased collagen, degenerative bony destruction and distribution of proteoglycans (PGs), safranin-O staining and Masson's trichrome staining were used.

측두하악관절에서 발생하는 골관절염은 저작시 통증, 개구제한, 관절잡음, 교합변화를 유발하여 환자의 삶의 질을 저하시키는데 관여하는 주요한 질환이다. 골관절염의 진행과정과 그에 따른 제어방법을 연구하기 위하여 다양한 연구방법이 시도되고 있다. 이 중 골조직면에서의 조직변화, 활막액의 성분변화, 활막의 조직변화, 주변조직의 변화, 통증에 대한 반응을 확인할 수 있는 동물모델이 주로 많이 이용된다. 본 연구에서는 실험동물에서 다양한 방법으로 측두하악관절 골관절염을 유도하여 하악관절표면에서 일어난 조직변화를 비교 관찰하였으며 각 동물모델의 연구 활용방안에 대하여 탐색하였다. 측두하악관절의 관절강에 monosodium iodoacetate (MIA) 와 interleukin-$1{\alpha}$(IL-$1{\alpha}$)를 직접 주입하여 약물로 관절염을 유도하여 관절조직면을 관찰하였다. 또한 여성호르몬인 에스트로겐에 의한 골변화를 확인하기 위하여 난소절제술로 인한 골다공증 유발모델에서의 하악관절을 관찰하였으며, 노화된 백서의 관절면을 확인함으로써 노화로 인한 골변화를 관찰하였다. Safranin-O 염색 법과 Masson's trichrome 염색법을 이용하여 각각의 관절면에서 세포배열의 변화, 콜라겐의 변화, 골조직의 변화 및 파괴양상에 대하여 확인하였고 Safranin-O의 양성반응을 확인하여 proteoglycans (PGs)의 조직내 분포를 추적하였다.

Keywords

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