Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
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Activities of Essential Oils from Perilla frutescens var. acuta against Antibiotic-Susceptible and -Resistant Vibrio and Salmonella Species

  • Received : 2011.08.26
  • Accepted : 2011.11.28
  • Published : 2011.12.30
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Abstract

We determined the inhibitory activity of the essential oil fraction obtained by steam distillation from the fresh and dried leaves of Perilla frutescens var. acuta against some pathogenic Salmonella and Vibrio spp. The activities of compounds isolated from the essential oils, apiol and myristicin, were also tested and the results were compared with those of the essential oil fraction. The Perilla essential oil fraction and its main components showed significant inhibition against antibiotic-susceptive and antibiotic-resistant strains of the tested Salmonella and Vibrio strains. Synergistic or additive effects were identified by combing the oils with ampicillin by checkerboard-titer tests. We conclude that essential oils from P. frutescens can be useful in the treatment of Salmonella and Vibrio infections and as safe additives to food materials for the prevention of contamination of food by these bacteria. This is especially important because of the rapid increase in antibiotic-resistant strains, which could cause severe symptoms in humans.

Keywords

References

  1. Andrysiak, A.K., Olson, A.B., Tracz, D.M., Dore, K., and Irvin, R., Genetic characterization of clinical and agri-food isolates of multi drug resistant Salmonella enteric serovar Heidelberg from Canada. BMC Microbiol. 8, 89-89 (2008). https://doi.org/10.1186/1471-2180-8-89
  2. Baker-Austin, C., Macarthur, J.V., Tuckfield, R.C., Najarro, M., Lindell, A.H., Gooch, J., and Stepanauskas, R., Antibiotic resistance in the shellfish pathogen Vibrio parahaemolyticus isolated from the coastal water and sediment of Georgia and South Carolina, USA. J. Food Protect. 71, 2552-2558 (2008). https://doi.org/10.4315/0362-028X-71.12.2552
  3. Benevides, P.J.C., Sartorelli, P., and Kato, M.J., Phenylpropanoids and neolignans from Piper regnellii. Phytochemistry 52, 339-343 (1999). https://doi.org/10.1016/S0031-9422(99)00177-6
  4. Chen G., Zhang, J., and Guo, Y., Analysis of volatile component of fresh Perilla frutescens (L) Britt. var. acuta (Thunb.) Kudo by headspace GC/MS. 2004. J. Essent. Oil Res. 16, 435-436 (2004). https://doi.org/10.1080/10412905.2004.9698765
  5. Choi, U.K., Lee, O.H., Lim, S.I., and Kim, Y.C., Optimization of antibacterial activity of Perilla frutescens var. acuta leaf against Pseudomonas aerunginosa using the evolutionary operation-factorial design technique. Int. J. Mol. Sci. 11, 3922-3932 (2010). https://doi.org/10.3390/ijms11103922
  6. Davidson, P.M., and Parish, M.E., Methods for testing the efficacy of food antimicrobials. Food Technol. 43, 148-155 (1989).
  7. Devi, R., Surendran, P.K. and Chkraborty, K., Antibiotic resistance and plasmid profiling of Vibrio parahaemolyticus isolated from shrimp farms along the southwest coast of India. World J. Microb.Biot. 25, 2005-2012 (2009). https://doi.org/10.1007/s11274-009-0101-8
  8. Hald, T., Lo Fo Wong, D.M.A., and Aarestrup, F.M., The attribution of human infections with antimicrobial resistant Salmonella bacteria in Denmark to sources of animal origin. Foodborne. Pathog. Dis. 4, 313-326 (2007). https://doi.org/10.1089/fpd.2007.0002
  9. Haldar, S., Mody, K.H., and Jha, B., Abundance, diversity and antibiotics resistance pattern of Vibrio spp. In coral ecosystem of Kursdai island. J. Basic Microb. 51, 163-162 (2011). https://doi.org/10.1002/jobm.201000160
  10. Huang, B., Lei, Y., Tang, Y., Zhang, J., Qin, L. and Liu, J., Comparison of HS-SPME with hydrodistillation and SFE for the analysis of the volatile compounds of Zisu and Baisu, two varietal species of Perilla frutescens of Chinese origin. Food Chem. 125, 268-275 (2011). https://doi.org/10.1016/j.foodchem.2010.08.043
  11. Kim, S.A., Seo, J.E., and Bae, J.H., Effect of Perilla frutescens extract on the growth of food-borne pathogens. J. East Asian Soc. Diat. Life 14, 472-478 (2004).
  12. Kitiyodom, S., Khemtong, S., Wongtavatchai, J., and Chuanchuen, R., Characterization of antibiotic resistance in Vibrio spp. Isolated from farmed marine shrimps (Penaeus monodon). FEMS Microbiol. Ecol. 72, 219-227 (2010). https://doi.org/10.1111/j.1574-6941.2010.00846.x
  13. Kitaoka, M., Miyata, S.T., Unterweger, D., and Pukatzki, S., Antibiotic resistance mechanisms of Vibrio cholerae. J. Med. Microbiol. 60, 397-407 (2011). https://doi.org/10.1099/jmm.0.023051-0
  14. Laureati, M., Buratti, S., Bassoli, A., Bortonovo, G., and Pagliarini, E., Discrimination and characterization of three cultivars of Perilla frutescens by means of sensory descriptors and electronic nose and tongue analysis. Food Res. Int. 43, 959-964 (2010). https://doi.org/10.1016/j.foodres.2010.01.024
  15. Lee, K.S., Lee, J.C., Han, K.H., and Oh, M.J., Antimicrobial activities of extracts of Perilla fruescens Briton var. acuta Kudo on food spoilage or foodborne disease microorganism. Kor. J. Postharvest Sci. 6, 239-244 (1999).
  16. Lee, S.H., Ahn, J.K., and Moon, H.I., Variations in the major essential oils content under different leaf-picking time of Perilla frutescens spp. Kor. J. Plant. Res. 10, 314-318 (1997).
  17. Liu, J.H., Steigel, A., Reininger, E., and Bauer, R., Two new prenylated 3-benzoxepin derivatives as cyclooxygenase inhibitors from Perilla frutescens var. acuta. J. Nat. Prod. 63, 403-405 (1999).
  18. Oh, E.K., Son, K.T., Yu, H., Lee, T.S., Lee, H.J., Shin. S., Kwon, J.Y., Park, K., and Kim, J., Antimicrobial resistance of Vibrio parahaemolyticus and Vibrio alginolyticus strains isolated from farmed fish in Korea from 2005 through 2007. J. Food Prot. 74, 380 (2011). https://doi.org/10.4315/0362-028X.JFP-10-307
  19. Ohk, H.C. and Chae, Y.A., Analysis of volatile oil components and identification of chemotypes in Jaso (Perilla frutescens) collected in Korea. Korean J. Medicinal Crop Sci. 12, 97-101 (2004).
  20. Rahim, N., Gomes, D.J., Watanabe, H., Rahman, S.R., Chomvarin, V., Endts, H.P., and Alam, M., Antibacterial activity of Psidium guajava leaf and bark against multidrug-reisstant Vibrio cholerae: implication for cholera control. JPN. J. Infect. Dis. 63, 271-274 (2010).
  21. Roig, F., Llorens, A., Fouz, B., and Amaro, C., Spontaneous quinolone resistance in the zoonotic serovar of Vibrio vulnificus. Appl. Environ. Microbiol. 75, 2577-2580 (2009). https://doi.org/10.1128/AEM.02921-08
  22. Shin, S., Studies on tissue cultures of Perilla species. Kor. J. Pharmacogn. 17, 7-11 (1986).
  23. Shin, S., In vitro effects of essential oils from Ostericum koreanum against antibiotic-resistant Salmonella species. Arch. Pharm. Res. 28, 765-769 (2005). https://doi.org/10.1007/BF02977340
  24. Shin, S., Antibacterial effects of vulgarone B from Artemisia iwaqomogi alone and in combination with oxacillin. Arch. Pharm, Res. 32, 1711-1719 (2009). https://doi.org/10.1007/s12272-009-2208-8
  25. Son, H.U., Heo, J.C., Seo, M.S., and Lee, S.H., Effects of Perilla frutescens L. on anti-oxidant and anti-inflammation activity. Korean J. Food Chem. 17, 757-761 (2010).
  26. Varma, J.K., Marcus, R., Stenzel, S.A., and Hanna, S.S., Highly resistant Salmonella Newport-MDRAmpC transmitted through the domestic US food supply: A FoodNet Case-Control Study of sporadic Salmonella newport infections, 2002-2003. J. Infect. Dis. 194, 222-230 (2006). https://doi.org/10.1086/505084
  27. Zhang, X., Wu, W., Zheng, Y., Chen, L., and Qianrong, C., Essential oil variation in different Perilla L. accessions: chemotaxonomic implication. Plant Sys. Evol. 281, 1-10 (2009). https://doi.org/10.1007/s00606-009-0152-1
  28. Zhu, Y.P., Chinese Materia Medica, Harwood Academic Publishers, Amsterdam, pp. 54-55, 1998.