Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Quantitative Analysis for the Quality Evaluation of Scutellariae Radix by HPLC/UVD

  • Received : 2011.08.11
  • Accepted : 2011.12.05
  • Published : 2011.12.30
Download PDF
⟨ Previous Next ⟩

Abstract

In this study, quantitative analysis was developed using HPLC/UVD for the quality evaluation of Scutellariae Radix. For quantitative analysis, six major bioactive compounds were assessed. The separation conditions employed for HPLC/UVD were optimized using Phenomenex $C_{18}$ column ($250{\times}4.6$ mm, 5 ${\mu}m$) with a gradient of solvent A (1% acetic acid of $H_2O$) and solvent B (acetonitrile : methanol : acetic acid = 70 : 30 : 1) as the mobile phase at a flow rate of 1.0 mL/min and a detection wavelength of 275 nm. These methods were fully validated with respect to linearity, accuracy, precision and recovery. The HPLC/UVD method was applied successfully to the quantification of six major compounds in the extract of Scutellariae Radix. The results indicate that the established HPLC/UVD method is suitable for the quantitative analysis and quality control of multicomponents in Scutellariae Radix.

Keywords

References

  1. Gao, Z.H., Huang, K.X., and Xu, H.B., Protective effects of flavonoids in the roots of Scutellaria baicalensis Georgi against hydrogen peroxide-induced oxidative stress in HS-SY5Y cells. Pharmacol. Res. 43, 173-178 (2001). https://doi.org/10.1006/phrs.2000.0761
  2. He, Z.Y., Cao, P.X., Liang, G.Y., Liu, Z.C., and Wu, X.G., Measurement of baicalin contents by HPLC. Zhongguo Zhongyao Zazhi 27, 258-260 (2002).
  3. Huang, R.L. Chen, C.C., Huang, H.L., Chang, C.G., Chen, C.F., Chang, and Hsieh, M.T., Anti-hepatitis B virus effects of wogonin isolated from Scutellaria baicalensis. Planta Med. 66, 694-698 (2000). https://doi.org/10.1055/s-2000-9775
  4. Ishimaru, K., Nishikawa, K., Omoto, T., Asai, I., Yoshihira, K., and Shimomura, K., Two flavone 2'-glucosides from Scutellaria baicalensis. Phytochemistry 40, 279-281 (1995). https://doi.org/10.1016/0031-9422(95)00200-Q
  5. Kimura, T., Ogita, H., and Ogita, Z., Effects of flavonoids isolated from Scutellariae radix on fibrinolytic system induced by trypsin in human umbilical vein endothelial cells. J. Nat. Prod. 60, 598-601 (1997). https://doi.org/10.1021/np970035l
  6. Koda, A., Watanabe, S., Yanagihara, Y., Nagai, H., and Sakamoto, K., A comparative study of the anti-allegic effects of disodium baicalein 6- phosphate (BPS) and disodium cromoglycate (DSCG). Jpn. J. Pharmacol. 27, 31-37 (1977). https://doi.org/10.1254/jjp.27.31
  7. Konoshima, T., Kokumai, M., Kozuka, M., Iinuma, M., Mizuno, M., Tanaka, T., Tokuda, H., Nishino, H., and Iwashima, A., Studies on inhibitors of skin tumor promotion. XI. inhibitory effects of flavonoids from Scutellaria baicalensis on epstein-barr virus activation and their anti-tumor-promoting activities. Chem. Pharm. Bull. 40, 531-533 (1992). https://doi.org/10.1248/cpb.40.531
  8. Li, B.Q., Fu, T., Gong, W.H., Dunlop, N., Kung, H.F., Yan, Y.D., Kang, J., and Wang, J.M., The flavonoid baicalin exhibits anti-inflammatory activity by binding to chemokines. Immunopharmacology 49, 295-306 (2000). https://doi.org/10.1016/S0162-3109(00)00244-7
  9. Li, J., Ding, Y.Q., Li, X.C., Ferreira, D., Khan, S., Smillie, T., and Khan, I.A., Scuteflorins A and B, dihydropyranocoumarins from Scutellaria lateriflora. J. Nat. Prod. 72, 983-987 (2009). https://doi.org/10.1021/np900068t
  10. Lu, Y., Joerger, R., and Wu, C., Study of the chemical composition and antimicrobial activities of ethanolic extracts from roots of Scutellaria baicalensis Georgi. J. Agric. Food Chem. 59, 10934-10942 (2011). https://doi.org/10.1021/jf202741x
  11. Ma, S.C., Du, J., But, P.P.H., Deng, X.L., Zhang, Y.W., Ooi, V.E.C., Xu, H.X., Lee, S.H.S., and Lee, S.F., Antiviral Chinese medicinal herbs against respiratory syncytial virus. J. Ethnopharmacol. 79, 205-211 (2002). https://doi.org/10.1016/S0378-8741(01)00389-0
  12. Coldwell, R.D., Trafford, D.J., Makin, H.L., and Varley, M.J., Specific mass fragmentographic assay for 25,26-dihydroxyvitamin D in human plasma using a deuterated internal standard. J. Chromatogr. 338, 289-302 (1985). https://doi.org/10.1016/0378-4347(85)80100-6
  13. Tomimori, T., Jin, H., Miyaichi, Y., Toyofuku, S., and Namba, T., Studies on the constituents of Scutellaria species. VI. on the flavonoid constituents of the root of Scutellaria baicalensis Georgi (5) quantitative analysis of flavonoids in Scutellaria roots by high-performance liquid chromatography. Yakugaku Zasshi 105, 148-155 (1985). https://doi.org/10.1248/yakushi1947.105.2_148
  14. Uchiyama, H., Mishima, K., Oka, S., Ezawa, M., Ide, M., and Park, P.W., Solubilities of flavone and 3-hydroxyflavone in supercritical carbon dioxide. J. Chem. Eng. Data 42, 570-573 (1997). https://doi.org/10.1021/je9603990
  15. Wu, J.A., Attele, A.S., Zhang, L., and Yuan, C.S., Anti-HIV activity of medicinal herbs: usage and potential development. Am. J. Chin. Med. 29, 69-81 (2001). https://doi.org/10.1142/S0192415X01000083
  16. Ye, F., Che, Y., Mcmillen, E., Gorski, J., Brodman, D., Saw, D., Jiang, B., and Zhang, D.Y., The effect of Scutellaria baicalensis on the signaling network on hepatocellular carcinoma cells. Nutr. Cancer 62, 530-537 (2009).
  17. Zhang, Y.Y., Don, H.Y., Guo, Y.Z., Ageta, H., Harigaya, Y., Onda, M., Hashimoto, K., Ikeya, Y., Okada, M., and Maruno, M., Comparative study of Scutellaria planipes and Scutellaria baicalensis. Biomed. Chromatogr. 12, 31-33 (1998). https://doi.org/10.1002/(SICI)1099-0801(199801/02)12:1<31::AID-BMC716>3.0.CO;2-S