Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Ameliorating Effects of Herbal Ethanol Extract from Gynostemma pentaphyllum on Chronic Stress-Induced Anxiety in Mice

돌외 에탄올 추출물 엑스의 만성 스트레스-유도 불안작용에 대한 개선작용

  • Received : 2010.12.03
  • Accepted : 2010.12.29
  • Published : 2011.03.31
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Abstract

The effects of herbal ethanol extract from Gynostemma pentaphyllum (GP extract) on chronic stress-induced anxiety in mice were investigated. The animals were treated with GP extracts (50 and 100 mg/kg/day, p.o.) for 21 days before exposure to electric footshock (EF; duration and interval 10 sec for 3 min, 2 mA) for chronic stress once a day. The ambulatory locomotor activity was reduced by chronic EF stress and it was recovered by 12.9-15.1% in GP extract-treated groups. The grip strength was also significantly decreased by chronic EF stress, however, the EF-stressed groups treated with GP extract increased grip strength from 13.9% to 56.8% compared to EF-stressed groups. In addition, the serum levels of corticosterone were significantly elevated by chronic EF stress to 197% of the control levels, which was reduced to 73.1% by treatment with GP extract (100 mg/kg). In contrast, the brain levels of dopamine and serotonin were reduced to 67.6% and 63.1% by chronic EF stress, which was recovered to 90% of the control levels by treatment with GP extract. These results indicate that GP extract shows the ameliorating effects on chronic EF stress-induced anxiety in mice and it can be developed as the promising anti-anxiety agent.

Keywords

References

  1. Yoshikawa, K., Mitake, M., Takemoto, T. and Arihara, S. (1987) Studies on the constituents of Curcubitaceae plants. XVII. On the saponins constituents of Gynostemma pentaphyllum Makino (12). Yakugaku. Zasshi 107: 355-360.
  2. Kuwahara, M., Kawanishi, F., Komiya, T. and Oshio, H. (1989) Dammarane saponins from Gynostemma pentaphylllum Makino and isolation of malonylginsenosides-$Rb_1$, -Rd, and malonylgypenoside V. Chem. Pharm. Bull. 37: 135-139. https://doi.org/10.1248/cpb.37.135
  3. Blumert, M. L. and Liu, J. (2003) Jiaogulan, China immortality herb, 12-20, Torchlight Publishing, Inc., Badger, CA, USA.
  4. Wang, Z. J. and Luo, D. H. (2007) Antioxidant activities of different fractions of polysaccharide purified from Gynostemma pentaphyllum Makino. Carbohyd. Polym. 68: 54-58. https://doi.org/10.1016/j.carbpol.2006.07.022
  5. Huang, T. H., Tran, V. H., Roufogalis, B. D. and Li, Y. (2007) Gypenoside XLIX, a naturally occurring PPAR-alpha activator, inhibits cytokine-induced vascular cell adhesion molecule-1 expression and activity in human endothelial cells. Eur. J. Pharmacol. 565: 158-165. https://doi.org/10.1016/j.ejphar.2007.03.013
  6. Circosta, C., De Pasquale, R. and Occhiuto, F. (2005) Cardiovascular effects of the aqueous extract of Gynostemma pentaphyllum Makino. Phytomedicine 12: 638-643. https://doi.org/10.1016/j.phymed.2004.06.023
  7. Megallia, S., Aktanb, Fugen., Daviesc, N. M. and Roufogalisa, B. D. (2005) Phytopreventative anti-hyperlipidemic effects of Gynostemma pentaphyllum in rats. J. Pharm. Pharmaceut. Sci. 8: 507-515.
  8. Huang, W. C., Kuo, M. L., Li, M. L., Yang, R. C., Liou, C. J. and Shen, J. J. (2007) The extract of Gynostemma pentaphyllum enhanced the production of antibodies and cytokines in mice. Yakugaku Zasshi 127: 889-896. https://doi.org/10.1248/yakushi.127.889
  9. Hou, J., Liu, S., MA, Z., Lang, X., Wang, J., Wang, J. and Liang, Z. (1991) Effects of Gynostemma pentaphyllum Makino on the immunological functions of cancer patients. J. Trad. Chin. Med. 11: 47-52.
  10. Norberg, A., Hoa, N. K., Liepinsh, E., Van Phan D., Thuan, N. D., Jornvall, H., Sillard, R. and Ostenson, C. G. (2004) A novel insulin-releasing substance, phanoside, from the plant Gynostemma pentaphyllum. J. Biol. Chem. 279: 41361-41367. https://doi.org/10.1074/jbc.M403435200
  11. Lin, J. M., Lin, C. C., Chiu, H. F., Yang, J. J. and Lee, S. G. (1993) Evaluation of the anti-inflammatory and liver-protective effects of Anoectochilus formosanus, Ganoderma lucidum and Gynostemma pentaphyllum in rats. Am. J. Chin. Med. 21: 59-69. https://doi.org/10.1142/S0192415X9300008X
  12. Rujjanawate, C., Kanjanapothi, D. and Amornlerdpison, D. (2004) The anti-gastric ulcer effect of Gynostemma pentaphyllum Makino. Phytomedicine 11: 431-435. https://doi.org/10.1016/j.phymed.2003.07.001
  13. Ulrich-Lai, P. M. and Engeland, W. C. (2005) Sympathoadrenal activity and hypothalamic-pituitary-adrenal axis regulation. In Steckler, T., Kalin, N. H., Reul, J. M. H. M. (ed.). Handbook of stress and the brain, Part I, 419-436, Elsevier.
  14. Gonzalo, A., Carrasco, L. D. and Van, D. K. (2003) Neuroendocrine pharmacology of stress. Eur. J. Pharmacol. 463: 235-272. https://doi.org/10.1016/S0014-2999(03)01285-8
  15. Kovacs, K. J., Mikios, I. H. and Bali, B. (2005) Psychological and physical stressors. In Steckler, T., Kalin, N. H., Reul, J. M. H. M. (ed.). Handbook of stress and the brain, Part I, 775-792, Elsevier.
  16. Retana-Marques, S., Bonilla-Jaime, H., Vazquez-Palacios, G., Dominguez-Salazar, E., Martinez-Garcia, R. and Velazquez-Moctezuma, J. (2003) Body weight gain and diurnal differences of corticosterone changes in response to acute and chronic stress in rats. Psychoneuroendocrinol. 28: 207-227. https://doi.org/10.1016/S0306-4530(02)00017-3
  17. Silva, M. I., de Aquino Neto, M. R., Teixeira Neto, P. F., Moura, B. A., do Amaral, J. F. and de Sousa, D. P. (2007) Central nervous system of acute administration of isopulegol in mice. Pharmacol. Biochem. Behav. 88: 141-147. https://doi.org/10.1016/j.pbb.2007.07.015
  18. Choi, H. S., Lim, S. A., Park, M. S., Hwang, B. Y., Lee, C. K., Kim, S. H., Lim, S. C. and Lee, M. K. (2008) Ameliorating effects of the ethanol extracts from Gynostemma pentaphyllum on electric footshock stress. Kor. J. Pharmacogn. 39: 341-346.
  19. Sheikh, N., Ahmad, A., Siripurapu, K. B., Kuchibhotla, V. K., Singh, S. and Palit, G. (2007) Effects of Bacopa monniera on stress induced changes in plasma corticosterone and brain monoamines in rats. J. Ethnopharmacol. 111: 671-676. https://doi.org/10.1016/j.jep.2007.01.025
  20. Niesenbaum, L. K. and Abercrombie, E. D. (1993) Presynaptic alterations associated with enhancement of evoked release and synthesis of norepinephrine in hippocampus of chronically cold stressed rats. Brain Res. 608: 280-287. https://doi.org/10.1016/0006-8993(93)91469-9
  21. Fujino, K., Yoshitake, T., Inoue, O., Ibii, N., Kehr, J., Ishida, J., Nohta, H. and Yamaguchi, M. (2002) Increased serotonin release in mice frontal cortex and hippocampus induced by acute physiological stressors. Neurosci. Lett. 320: 91-95. https://doi.org/10.1016/S0304-3940(02)00029-0
  22. Lim, S. A., Choi, H. S., Hwang, B. Y., Lee, M. K. and Lee, C. K. (2009) Augmentation of immune responses by oral administration of Gynostemma pentaphyllum ethanol extract. Kor. J. Pharmacogn. 40: 35-40.
  23. Mitsui, A., Nohta, H. and Ohkura, Y. (1985) High-performance liquid chromatography of plasma catecholamines using 1,2-diphenylethylenediamine as precolumn fluorescence derivatization reagent. J. Chromatogr. 344: 61-70.
  24. Yanagisa, M., Hasegawa, H. and Ichiyama, A. (1982) Assay of tryptophan hydroxylase and aromatic L-amino acid decarboxylase based on rapid separation of the reaction product by high-performance liquid chromatography. J. Biochem., 92: 449-456.
  25. Attawish, A., Chivapat, S., Phadungpat, S., Bansiddhi, J., Techadamrongsin, Y., Mitrijit, O., Chaorai, B. and Chavalittumrong, P. (2004) Chronic toxicity of Gynostemma pentaphyllum. Fitoterapia 75: 539-551. https://doi.org/10.1016/j.fitote.2004.04.010
  26. Akana, S. F., Shinsako, J. and Dallman, M. F. (1983) Relationship among adrenal weight, corticosterone and stimulated adrenocorticotropin. Endocrinol. 113: 2226-2239. https://doi.org/10.1210/endo-113-6-2226
  27. Piemonti, L., Monti, P., Allavena, P., Sironi, M., Soldini, L., Leone, B. E., Socci, C. and Carlo, V. (1999) Glucocorticoids affect human dendritic cell differentiation and maturation. J. Immunol. 162: 6473-6481.
  28. Tuckermann, J. P., Kleiman, A., Moriggl, R., Spanbroek, R., Neumann, A., Illing, A., Clausen, B. E., Stride, B., Forster, I., Habenicht, A. J., Reichardt, H. M., Tronche, F., Schmid, W. and Schutz, G. (2007) Macrophages and neutrophils are the targets for immune suppression by glucocorticoids in contact allergy. J. Clin. Invest. 117: 1381-1390. https://doi.org/10.1172/JCI28034
  29. Choi, H. S., Park, M. S., Kim, S. H., Hwang, B. Y., Lee, C. K. and Lee, M. K. (2010) Neuroprotective effects of herbal ethanol extracts from Gynostemma pentaphyllum in the 6-hydroxydopamine-lesioned rat model of Parkinson's disease. Molecules 15: 2814-2824. https://doi.org/10.3390/molecules15042814