Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
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Evaluation of the Antimicrobial Activity of Seven Gabonese Medicinal Plants against Methicillin-Resistant Staphylococcus aureus and Salmonella

  • Obiang-Obounou, Brice W. (College of Pharmacy and Wonkwang-Oriental Medicines Research Institute, Wonkwang University) ;
  • Kang, Ok-Hwa (College of Pharmacy and Wonkwang-Oriental Medicines Research Institute, Wonkwang University) ;
  • Choi, Jang-Gi (College of Pharmacy and Wonkwang-Oriental Medicines Research Institute, Wonkwang University) ;
  • Keum, Joon-Ho (College of Pharmacy and Wonkwang-Oriental Medicines Research Institute, Wonkwang University) ;
  • Kim, Sung-Bae (College of Pharmacy and Wonkwang-Oriental Medicines Research Institute, Wonkwang University) ;
  • Kim, Yong-Sik (College of Pharmacy and Wonkwang-Oriental Medicines Research Institute, Wonkwang University) ;
  • Mun, Su-Hyun (College of Pharmacy and Wonkwang-Oriental Medicines Research Institute, Wonkwang University) ;
  • Choi, Mi-Sun (College of Pharmacy and Wonkwang-Oriental Medicines Research Institute, Wonkwang University) ;
  • Maroufath, Lamidi (IPHAMETRA (Institute of Traditional Pharmacopoeia and Medicine), CENAREST) ;
  • Kwon, Dong-Yeul (College of Pharmacy and Wonkwang-Oriental Medicines Research Institute, Wonkwang University)
  • Received : 2010.11.11
  • Accepted : 2011.03.05
  • Published : 2011.03.31
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Abstract

The plant species reported here are used by traditional healers in Gabon for different ailments such as wounds, malaria, fever, gonorrhea or diarrhea. The aim of this study was to evaluate the antimicrobial activities of 7 plants (Strombosiopsis tetrandra, Tetraberlinia bifoliolata, Dichapetalum barbatum, Guibourtia demeusii, Dacryodes normandii, Manniophytum fulvum, Paropsia grewoides) against different strains of both Methicillin-Resistant Staphylococcus aureus (MRSA) and Salmonella. Disc diffusion was first used to determine the antimicrobial effectiveness of the plants' ethanolic extracts. Then the minimum inhibitory concentrations of the crude extracts of either leaves or stem barks of the 7 plants were determined using broth micro-dilution. The ethanolic plant extracts showed very good activity against both MRSA and Salmonella strains where the MICs ranged from $250\;{\mu}g/ml$ to $1000\;{\mu}g/ml$. The study shows that many of the tested plants used by Gabonese traditional healers have antimicrobial activities and give support to their traditional use.

Keywords

References

  1. Adjuik, M., Babiker, A., and White, N., Combinations for treatment of malaria: meta analysis. Lancet. 363, 9-17 (2004). https://doi.org/10.1016/S0140-6736(03)15162-8
  2. Akinyemi, K.O., Oladapo, O., and Fasure, K.A., Screening of crude extracts of six medicinal plants used in South-West Nigerian unorthodox medicine for anti-methicillin resistant Staphylococcus aureus activity. BMC complement altern med. 5, 6 (2005). https://doi.org/10.1186/1472-6882-5-6
  3. Amoo, S.O., Finnie, J.F., and Van, S.J., In vitro pharmacological evaluation of three Barleria species. J ethnopharmacol. 121(2), 274-277 (2009). https://doi.org/10.1016/j.jep.2008.10.035
  4. Cabrera, R., Ruiz, J., Usera, Vila, J., Mechanism of resistance to several antimicrobial agents in Salmonella Clinical isolates causing traveler's diarrhea. Antimicrob agents chemother. 48(10), 3934-3939 (2004). https://doi.org/10.1128/AAC.48.10.3934-3939.2004
  5. Chea, A., jonville, M.C., and Balansard, G., In vitro Antimicrobial Activity of Plants used in Cambodian Traditional Medicine., Am J Chin Med. 35(5), 867-873 (2007). https://doi.org/10.1142/S0192415X07005338
  6. Choi, J.G., Kang, O.H., and Kwon, D.Y., Antibacterial Activity of Hylomecon hylomeconoides Against Methicillin-Resistant Staphylococcus aureus. Appl Biochem Biotechnol. 160(8), 2467-2474 (2010). https://doi.org/10.1007/s12010-009-8698-5
  7. Cowan, M., Plant Products as Antimicrobial Agents. Clin Microbiol Rev. 564-582 (1999).
  8. Cloutier, M.J., Antibiotics: Mechanisms of Action and the Acquisition of Resistance: When Magic Bullets Lose Their Magic. Am J Pharm Educ. 59 (1995).
  9. CLSI., Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. Approved standards. CLSI document M7-A5. Wayne, PA (2000).
  10. Elujoba, A.A., Odeleye, O.M., and Ogunyemi, C.M., Traditional Medicine Development for medical and dental primary health care delivery system in dental primary health Care Delivery system in Africa. Afri. J. Trad. 2(1), 46-61 (2005).
  11. Farombi, E., Olatunde., African indigenous plants with chemotherapeutic potentials and biotechnological approach to the production of bioactive prophylactic agents. AJB. 2(12), 662-671 (2003).
  12. Fink, S.L. and Cookson, B.T., Pyroptosis and host cell death responses during Salmonella infection. Cell Microbiol. 9, 2562-2570 (2007). https://doi.org/10.1111/j.1462-5822.2007.01036.x
  13. Garcia, D., Leiro, J., and Ubeira, F.M., Mangifera indica L. extract (Vimang) and mangiferin modulate mouse humoral immune responses. Phytother Res. 17(10), 1182-1187 (2003). https://doi.org/10.1002/ptr.1338
  14. Grassl, G.A., Valdez, Y., and Finlay, B.B., Chronic enteric Salmonella infection in mice leads to severe and persistent intestinal fibrosis. Gastroenterology. 134, 768-780 (2008). https://doi.org/10.1053/j.gastro.2007.12.043
  15. Joung, H. and Shin, D.W., Antibacterial and synergistic effects of Smallanthus sonchifolius leaf extracts against methicillin-resistant Staphylococcus aureus under light intensity. J Nat Med. 64, 212-215 (2010). https://doi.org/10.1007/s11418-010-0388-7
  16. Himal, P.C., Nisha, S.Y., and Panna, T., Phytochemical and antimicrobial evaluation of Medicinal plants of Nepal. KUSET. 5, 49-54 (2008).
  17. Lamidi, M., DiGiorgio, C., Delmas, F., and Balansard, G., In vitro cytotoxic, antileishmanial and antifungal activities of ethnopharmacologically selected Gabonese plants. J Ethnopharmacol. 102(2), 185-190 (2005). https://doi.org/10.1016/j.jep.2005.06.011
  18. Lewis, K. and Ausubel, F., Prospects for plant-derived antibacterials. Nat Biotechnol. 24(12), 1504-1507 (2006). https://doi.org/10.1038/nbt1206-1504
  19. Mills, E., Cooper, C., and Kanfer, I., African herbal medicines in the treatment of HIV: Hypoxis and Sutherlandia. An overview of evidence pharmacology. Nutr J. 4, 19 (2005). https://doi.org/10.1186/1475-2891-4-19
  20. Mothana, R.A., Abdo, S.A., and Lindequist, U., Antimicrobial, Antioxidant and Cytotoxic Activities and Phytochemical Screening of Some Yemeni Medicinal Plants. Evid based complement alternat Med (2008).
  21. Rabe, T. and Van, S. J., Antibacterial activity of South African plants used for medicinal purposes. J Ethnopharmacol. 56(1), 81-87 (1997). https://doi.org/10.1016/S0378-8741(96)01515-2
  22. Samy, R.P. and Gopalakrishnakone, P., Therapeutic Potential of Plants as Anti-microbials for Drug Discovery. Evid based complement alternat med. (2008).
  23. Tenover, F., Mechanisms of Antimicrobial Resistance in Bacteria. Am J Med. 119(6A), S3-S10 (2006).