KOREAN JOURNAL OF CROP SCIENCE (한국작물학회지)

The Korean Society of Crop Science (한국작물학회)
권호 표지
DOI QR코드

DOI QR Code

Genetic Variation of Foxtail Millet [Setaria italica (L.) P. Beauv.] Among Accessions Collected From Korea Revealed by AFLP Markers

AFLP 분자마커를 이용한 우리나라에서 수집한 조 계통들의 유전적 다양성

  • Kim, Eun-Ji (Department of Applied Plant Sciences, College of Agriculture and Life Sciences, Kangwon National University) ;
  • Sa, Kyu-Jin (Department of Applied Plant Sciences, College of Agriculture and Life Sciences, Kangwon National University) ;
  • Lee, Ju-Kyong (Department of Applied Plant Sciences, College of Agriculture and Life Sciences, Kangwon National University)
  • 김은지 (강원대학교 농업생명과학대학 식물자원응용공학과) ;
  • 사규진 (강원대학교 농업생명과학대학 식물자원응용공학과) ;
  • 이주경 (강원대학교 농업생명과학대학 식물자원응용공학과)
  • Received : 2011.10.26
  • Accepted : 2011.11.11
  • Published : 2011.12.30
Download PDF
⟨ Previous Next ⟩

Abstract

AFLP markers were employed to detect genetic diversity and genetic relationship among 26 accessions of foxtail millet collected from Korea. Analysis of 26 accessions of foxtail millet with nine AFLP primer set combinations detected a total of 170 bands, of which 145 (85.3%) showed polymorphic at the species level. The phenotypic diversity (Hs) calculated for the nine primer combinations ranged from 1.84 to 6.8, with an average of 3.85. The average phenotypic diversity values were 3.39 and 2.99 for accessions collected from Gangwon province (Group 1), and accessions collected from the other areas including Gyeonggi province (Group 2), respectively. In the cluster analysis of 26 accessions, two major groups were recognized at 73% genetic similarity. Group I includes 13 accessions, which were collected in Gangwon province, and 1 accession, which was collected in Gyeonggi province, with genetic similarity of 76.8%. Group II includes two accessions, which were collected in Gangwon province, and 10 accessions, which were collected in the other areas with genetic similarity of 78.9%. The presenting data on the assessment of genetic diversity and genetic relationships among Korean accessions of foxtail millet will be helpful for efficient collection or conservation strategy of foxtail millet germplasm in Korea.

우리나라에서 수집한 재래종 조 26계통들에 대하여 9개의 AFLP primer조합을 사용하여 유전적 다양성 및 계통유연관계를 분석한 결과는 다음과 같다. 1. 분석에 이용한 9개의 AFLP primer조합들은 국내에서 수집한 조 26계통들에서 총 170개의 DNA단편을 나타내었고, 이중에서 145개의 단편(85.3%)들은 다형성을 나타내었다. 9개의 AFLP primer조합들에서 측정된 유전적 다양성 값(Hs)은 1.84에서 6.80의 범위로 나타나, 평균 3.85값을 나타내었다. 강원지역에서 수집한 조 계통(Group 1)들은 평균 3.39의 유전적 다양성 값을 나타내었고, 경기도 등 타 지역에서 수집한 조 계통들(Group 2)은 평균 2.99의 유전적 다양성 값을 나타내었다. 2. 국내에서 수집된 재래종 조 26계통들은 유전적 유사성 73% 수준에서 크게 두 개의 그룹으로 구분되었다. Group I은 유전적 유사성 76.8% 수준에서 강원도에서 수집한 13계통들과 경기도에서 수집한 1계통을 포함하고 있었으며, Group II는 유전적 유사성 78.9% 수준에서 강원도에서 수집한 2계통들과 타 지역에서 수집한 10계통들을 포함하고 있었다. 본 연구결과는 우리나라에서 수집한 재래종 조 계통들에 대한 유전적 다양성 및 계통유연관계 이해 그리고 이들 자원의 수집 및 보존 등에 유용한 정보를 제공할 것으로 기대된다.

Keywords

References

  1. Bowman, K. D., K. Hutcheson, E. P. Odum and L. R. Shenton. 1971. Comments on the distribution of indices of diversity. Stat. Ecol. 3 : 315-359.
  2. Chen, Y. and R. L. Nelson. 2004. Genetic variation and relationships among cultivated, wild and semiwild soybean. Crop sci. 44 : 316-325. https://doi.org/10.2135/cropsci2004.0316
  3. Cho, Y. S., S. K. Hong, M. T. Song, H. P. Moon, J. H. Lee, and N. S. Kim. 1998. Comparison of genetic variation among rice varieties detected by RAPD, AFLP and SSRP. Korea J. Genetic 20 : 117-127.
  4. De wet J. M. J., L. L. Oestry-Stidd and J. I. Cubero. 1979. Origins and evolution of foxtail millets (Setaria italica) J. Agric. Trop. Bot. Appl. [JATBA] 26 : 53-64.
  5. Dellaporta S. L., J. Wood and J. B. Hicks. 1983. A plant DNA minipreparation: Version II. Plant Mol. Biol. Rept. 1 : 19-21. https://doi.org/10.1007/BF02712670
  6. Dice L. R. 1945. Measures of the amount of ecologic association between species. Ecology 26 : 297-302. https://doi.org/10.2307/1932409
  7. Fukunaga K., E. Domon and M. Kawase. 1997. Ribosomal DNA variation in foxtail millet, Setaria italica (L.) P. Beauv. and a survey of variation from Europe and Asia. Theor. Appl. Genet. 95 : 751-756. https://doi.org/10.1007/s001220050621
  8. Fukunaga K., Z. M. Wang, K. Kato and M. Kawase. 2002. Geographical variation of nuclear genome RFLPs and genetic differentiation in foxtail millet, Setaria italica (L.) P. Beauv. Genet. Resour. Crop. Evol. 49 : 95-101. https://doi.org/10.1023/A:1013852007770
  9. Garcia-Mas, J., M. Oliver, H. Gomez-Paniagua, and M. C. de Vicente. 2000. Comparing AFLP, RAPD and RFLP markers for measuring genetic diversity in melon. Theor. Appl. Genet. 101 : 860-864.
  10. Harlan J. R. 1995. The living fields: our agricultural heritage. Cambridge University Press, Cambridge.
  11. Jia, X., Z. Zhang, Y. Liu, C. Zhang, Y. Shi, Y. Song, T. Wang and Y. Li. 2009. Development and genetic mapping of SSR markers in foxtail millet (Setaria italica (L.) P. Beauv.). Theor. Appl. Genet. 118 : 821-829. https://doi.org/10.1007/s00122-008-0942-9
  12. Jusuf M. and J. Pernes. 1985. Genetic variability of foxtail millet (Setaria italica. (L.) P. Beauv): Electrophoretic study of five isozyme systems. Theor. Appl. Genet. 71 : 385-391.
  13. Kawase M and S. Sakamoto. 1984. Variation, geographical distribution and genetic analysis of esterase isozymes in foxtail millet, Setaria italica (L.) P. Beauv. Theor. Appl. Genet. 67 : 529-533. https://doi.org/10.1007/BF00264899
  14. Kihara H. and E. Kishimoto. 1942. Bastarde zwischen Setaria italica and Setaria viridis. Bot. Mag. pp62-67.
  15. Kim S. K., E. Y. Sohn and I. J. Lee. 2009. Starch properties of native foxtail millet, Setaria italica Beauv. J. Crop. Sci. Biotech. 12(1) : 59-62. https://doi.org/10.1007/s12892-009-0073-0
  16. Le T. D., O. Penaud, B. Toupance and A. Sarr. 2000. Assessment of genetic relationships between Setarica italica and its wild relative S. viridis using AFLP marker. Theor. Appl. Genet. 100 : 1061-1066. https://doi.org/10.1007/s001220051387
  17. Li C. H., W. K. Pao and H. W. Li. 1942. Interspecific crosses in Setaria II, Cytological studies of interspecific hybrids involving : 1, S. faberi and S. italica, and 2, a three way cross, F2 of S. italica ${\times}$ S. viridis and S. faberi. J. Hered. 33 : 351-355.
  18. Li, Y., Y. S. Cao, S. Z. Wu and X. Z. Zhang. 1995. A diversity analysis of foxtail millet (Setaria italica (L.) P. Beauv) landraces of Chinese origin. Genet. Res. Crop. Evol. 45 : 279-285.
  19. Li, Y., J. Jia, Y. Wang and S. Wu. 1998. Intraspecific and interspecific variation in Setaria revealed by RAPD analysis. Genet. Res. Crop. Evol. 45 : 279-285. https://doi.org/10.1023/A:1008600123509
  20. Lu, T. L. D. 2002. A green foxtail (Setaria viridis) cultivation experiment in the middle Yellow River valley and some related issues. Asian Perspect. 41 : 1-14. https://doi.org/10.1353/asi.2002.0007
  21. Marathee, J. P. 1993. Structure and characteristics of the world millet economy. p. 159-178. In: Advances in small millets (Riley KW, Gupta SC, Seetharam A, Mushonga JN, eds.). Oxford & IBH press, New Delhi, India.
  22. Rohlf, F. J. 1998. NTSYS-pc numerical taxonomy and multivariate analysis system, version 2.02. Exeter Software, Setauket, New York.
  23. Sakamoto, S. 1987. Origin and dispersal of common millet and foxtail millet. JARQ 21(22) : 84-89.
  24. Sakamoto, S. 1988. Zakkoku no kita michi (The road of Millets): Eurasia no minzoku shokudutsu-gaku kara (from the ethnobotanical study in Eurasi). Japan Broadcast Publishing, Tokyo (in Japan).
  25. Schontz, D. and B. Rether. 1998. Genetic variability in foxtail millet, Setaria italica (L.) P. Beauv. RFLP using a heterologous rDNA probe. Plant Breed. 117 : 231-234. https://doi.org/10.1111/j.1439-0523.1998.tb01931.x
  26. Schontz, D. and B. Rether. 1999. Genetic variability in foxtail millet, Setaria italica (L.) P. Beauv. : Identification and classification of lines with RAPD markers. Plant Breed. 118 : 190-192. https://doi.org/10.1046/j.1439-0523.1999.118002190.x
  27. Vavilov, N. I. 1926. Studies on the origin of cultivated plants. Inst. Appl. Bot. Plant Breed. 16 : 1-248.
  28. Vos, P., R. Hogers, M. Bleeker, M. Reijans, T. van de Lee, M. Hornes et al. 1995. AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res. 23 : 4407-4414. https://doi.org/10.1093/nar/23.21.4407
  29. Wachira, F. N., R. Waugh, W. Powell, and C. A. Hacket. 1995. Detection of genetic diversity in tea (Camellia sinensis) using RAPD markers. Genome 38 : 201-210. https://doi.org/10.1139/g95-025
  30. Zabeau, M. and P. Vos. 1993. Selective restriction fragment amplification: a general method for DNA fingerprinting. European Patent Application 92402629.7 (Publ. No. 0534858Al).
  31. Zhao, J., X. Wang, B. Deng, P. Lou, J. Wu, R. Sun, Z. Xu, J. Vromans, M. Koornnef, and G. Bennema. 2005. Genetic relationship within Brassica rapa as inferred from AFLP fingerprints. Theor. Appl Genet. 110 : 1301-1314. https://doi.org/10.1007/s00122-005-1967-y

Cited by

  1. Study of genetic diversity and relationships among accessions of foxtail millet [Setaria italica (L.) P. Beauv.] in Korea, China, and Pakistan using SSR markers vol.34, pp.5, 2012, https://doi.org/10.1007/s13258-012-0074-0
  2. Milling Condition and Harvesting Time for Improving Milling Recovery of Head Foxtail Millet Grain vol.57, pp.3, 2012, https://doi.org/10.7740/kjcs.2012.57.3.280
  3. EST-SSR Based Genetic Diversity and Population Structure among Korean Landraces of Foxtail Millet (Setaria italica L.) vol.29, pp.3, 2016, https://doi.org/10.7732/kjpr.2016.29.3.322
  4. Phylogenetic relationships among species of Setaria (Paniceae; Panicoideae; Poaceae) in Korea: insights from nuclear (ITS and kn1) and chloroplast DNA sequence data vol.301, pp.2, 2015, https://doi.org/10.1007/s00606-014-1111-z
  5. Identification of Populations of Korean Mistletoe (Viscum AlbumL. Var.Coloratum) from Gangwon-Do in Korea by AFLP vol.27, pp.5, 2013, https://doi.org/10.5504/BBEQ.2013.0035