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Physicochemical Properties of a Giant Embryo Mutant Induced by T-DNA Insertion in Rice

  • Park, Hee-Yeon (Department of Agronomy, Kyungpook National University) ;
  • Qin, Yang (Biosafety Division, National Academy of Agricultural Science, RDA) ;
  • Sohn, Jae-Keun (Department of Agronomy, Kyungpook National University)
  • 투고 : 2011.09.15
  • 심사 : 2011.12.20
  • 발행 : 2011.12.30

초록

This study was conducted to determine the physicochemical properties of a giant embryo rice 'P47JB-4-B-5-B' derived from the cross between 'P47', a mutant of 'Hwayoung' induced by T-DNA insertion, and 'Junam'. The grain appearance and chemical components of the embryo were analyzed and compared with a donor cultivar, 'Hwayoung'. The proportion of embryo weight to grain weight of 'P47 JB-4-B-5-B' was 2.2 times heavier (6.7%) than that (3.1%) of 'Hwayoung'. Total free amino acid content (75.81 mg/100 g) of 'P47JB-4-B-5-B' was 2.1 times higher than that of 'Hwayoung'. The GABA content in brown rice was 14.06 mg/100 g in 'P47JB-4-B-5-B' and 6.8 mg/100 g in 'Hwayoung'. Especially, the GABA content in brown rice of 'P47JB-4-B-5-B' remarkably increased (about 33 times from 1.48 mg to 44.81 mg/100 g) 2 days after germination. Continuous frequency distributions and transgressive segregation in embryo length and width were observed in the $F_2$ population of the cross between 'P47' and 'Cheongcheong', indicating that the giant embryo was controlled by quantitative trait loci. However, embryo length and width demonstrated high broad sense heritability, implying that giant embryonic traits could be selected in earlier generations in comparison with other quantitative traits.

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참고문헌

  1. Choi, I. D., Kim, D. S., Son, J. R., Yang, C. G., Chun, J. Y. and Kim, K. J. 2006. Physico-chemical properties of giant embryo brown rice (Keuunnunbyeo). Agric. Chem. Biotechnol. 49(3) : 95-100.
  2. Choi, I. D., Suh, S. J., Kim, J. H. and Kim, S. L. 2009. Effects of germination on fatty acid and free amino acid profiles of brown rice 'Keunnun'. Food Sci. Biotechnol. 18(3) : 799-802.
  3. Choi, H. C. 2005. Current status of varietal improvement and use of specially rice in Korea. Rice is life: scientific perspectives for the 21st century. Session 9: Developing new uses of rice : 272-275.
  4. Choi, H. D., Park, Y. K. and Kim, Y. S. 2004. Effect of pretreatment conditions on γ-aminobutyric acid content of brown rice and germinated brown rice. Korean J. Food Sci. Technol. 36(5) : 761-764.
  5. Dong, Y. J., Tsuzuki, E., Kanmiunten, H., Terad, H. and Lin, D. Z. 2003. Mapping of QTL for embryo size in rice. Crop Sci. 43 : 1068-1071. https://doi.org/10.2135/cropsci2003.1068
  6. Fusino, Y. 1978. Rice lipid. Cereal Chem 55 : 559-571.
  7. Hinton, J. J. C. 1948. The distribution of vitamin B1 in the rice grain. Br. J. Nutr. 2 : 237-241. https://doi.org/10.1079/BJN19480041
  8. Hong, S. K., Kitano, H., Satoh, H. and Nagato, Y. 1995. Mutations affecting embryo size in rice. Rice Genetics Newsletter 12.
  9. Hong, S. K. 2002. Developmental characterization of embryo size mutant in rice (Oryza sativa L.). Korean J. Plant. Res. 5(2) : 141-154.
  10. Hong, S. K. 2002. Interaction among genes related to embryo size in rice. Korean J. Breed 34(3) : 212-222.
  11. Jannoey, P., Niamsup, H., Lumyong, S., Tajima, S., Nomura, M. and Chairote, G. 2010. γ-aminobutyric acid (GABA) accumulations in rice during germination. Chiang Mai J. Sci. 37(1) : 124-133.
  12. Jeon, J. S., Lee, S. C., Jung, K. H., Jun, S. H., Jeong, D. H., Lee, J. W., Kim, C. H., Jang, S. H., Lee, S. Y., Yang, K. Y., Nam, J. M., An, K. S., Han, M. J., Sung, R. J., Choi, H. S., Yu, J. H., Choi, J. H., Cho, S. Y., Cha, S. S., Kim, S. I. and An, G. H. 2000. T-DNA insertional mutagenesis for functional genomics in rice. The Plant Journal 22(6) : 561-570. https://doi.org/10.1046/j.1365-313x.2000.00767.x
  13. Kang, T. J. and Oh, S. H. 2007. GABA production and uses. BioWave (http://bric.postech.ac.kr/webzine) 9(14).
  14. Kien, T. H., Oh, J. M., Yang, P., Hong, S. K. and Ahn, S. N. 2010. Analysis and mapping of the re-1 gene for reduced embryo size in rice. Korean J. Breed. Sci. 42(1) : 23-27.
  15. Kim, K. H., Heu, M. H., Park, S. Z. and Koh, H. J. 1991. New mutants for endosperm and embryo characters in rice. Korean J. Crop Sci 36(3) : 197-203.
  16. Koh, H. J., Park, S. Z., Won, Y. J. and Heu, M. H. 1993. Nutritional and agronomic characteristics of super-giant embryo mutant in rice. Korean J. Crop Sci. 38(6) : 537-544.
  17. Koh, H. J., Myung, J. S., Heu, M. H. and McCouch, S. R. 1996. Tagging of ges gene controlling the super-giant embryo character using RFLP, RAPD, and Microsatellite markers in rice. Korean J. Breed. 28(4) : 436-444.
  18. Koh, H. J., Heu, M. J. and McCouch, S. R. 1996. Molecular mapping of the ges gene controlling the super-giant embryo character in rice (Oryza sativa L.). Theor Appl Genet 93 : 257-261. https://doi.org/10.1007/BF00225754
  19. Lin, Y. R., Chiang, S. H. and Wu, Y. P. 2009. Fine mapping of the giant embryo gene GE2 in rice. International Rice Research in the Era of Glabal Warning 17-30.
  20. Maeda, H., Nemoto, H., Iida, S., Ishii, T., Nakagawa, N., Hoshino, T., Sakai, M., Okamoto, M., Shinoda, H. and Yoshida, T. 2001. A new rice variety with giant embryos, "Haiminori". Breeding Science 51 : 211-213. https://doi.org/10.1270/jsbbs.51.211
  21. Nagasawa, K. and Onota, A. 1996. Accumulation of gammaaminobutyric acid (GABA) in the rice germ. Food Process 31 : 43-46.
  22. Oh, S. K., Hwang, P. S., Kim, K. G., Kim, Y. K. and Lee, J. H. 2010. Changes in nutritional components throughout germination in paddy rice and brown rice. J Food Sci Nutr 15 : 113-119. https://doi.org/10.3746/jfn.2010.15.2.113
  23. Park, D. S., Park, S. K., Lee, B. C., Song, S. Y., Jun, N. S., Manigbas, N., Cho, J. H., Nam, M. H., Jeon, J. S., Han, C. D., Choi, K. J., Kim, D. H., Woo, Y. M., Koh, H. J., Kang, H. W. and Yi, G. H. 2009. Molecular Characterization and physico-chemical analysis of a new giant embryo mutant allele (get) in ric (Oryza sativa L.). GENES & GENOMICS 31(4) : 277-282. https://doi.org/10.1007/BF03191200
  24. Qian, Q., Xiong, Z., Min, S. and Zhu, L. H. 1996. The RFLP tagging of giant embryo gene. Chinese J. Rice Sci. 10 : 65-70.
  25. Qin, Y., Kim, S. M., Park, H. Y. and Sohn, J. K. 2009. Inheritance analysis of giant embryo mutation by T-DNA insertion in Rice. Korea J. Breed. Sci. 41(1) : 9-15.
  26. Saikusa, T., Horino, T. and Mori, Y. 1994. Distribution of free amino acids in rice kernel and kernel fractions and the effect of water soaking on the distribution. J. Agric Food Chem. 42 : 1122-1125. https://doi.org/10.1021/jf00041a015
  27. Satoh, H. and Omura, T. 1981. New endosperm mutations induced by chemical mutagens in rice (Oryza sativa L.). Japan J. Breed. 31(3) : 316-326. https://doi.org/10.1270/jsbbs1951.31.316
  28. Zhang, L. L., Hu, P. S., Tang, S. Q., Zhao, H. J. and Wu, D. Z. 2005. Comparative studies on major nutritional components of rice with a giant embryo and a normal embryo. J Food Biochem. 29(6) : 653-661. https://doi.org/10.1111/j.1745-4514.2005.00039.x
  29. Zhang, H., Yao, H. Y., and Chen, F. 2006. Accumulation of gamma-aminobutyric acid in rice germ Protease. Biosci. Biotechnol Biochem. 70 : 1160-1165. https://doi.org/10.1271/bbb.70.1160