Animal cells and systems

  • 제15권2호
  • /
  • Pages.107-114
  • /
  • 2011
  • /
  • 1976-8354(pISSN)
  • /
  • 2151-2485(eISSN)
한국통합생물학회 (The Korean Society for Integrative Biology)
권호 표지
DOI QR코드

DOI QR Code

Toxicoproteomic identification of $TiO_2$ nanoparticle-induced protein expression changes in mouse brain

  • Jeon, Yu-Mi (Department of Medical Biotechnology, SoonChunHyang University) ;
  • Park, Seul-Ki (Department of Medical Biotechnology, SoonChunHyang University) ;
  • Lee, Mi-Young (Department of Medical Biotechnology, SoonChunHyang University)
  • 투고 : 2010.08.26
  • 심사 : 2010.10.24
  • 발행 : 2011.06.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

A proteomic analysis of the proteins in mouse brain that were differentially expressed in response to $TiO_2$ nanoparticles was conducted to better understand the molecular mechanism of $TiO_2$ nanoparticle-induced brain toxicity at the protein level. A total of 990 proteins from mouse brain were resolved by two-dimensional gel electrophoresis. A comparative proteomic analysis revealed that the expression levels of 11 proteins were changed by more than 2-fold in response to $TiO_2$ nanoparticles: eight proteins were upregulated and three were downregulated by $TiO_2$ nanoparticles. In addition, the activities of several antioxidative enzymes and acetylcholine esterase were reduced in $TiO_2$ nanoparticle-exposed mouse brain. The protein profile alterations seem to be due to an indirect effect of $TiO_2$ nanoparticles, because $TiO_2$ nanoparticles were not detected in the brain in this investigation.

키워드

참고문헌

  1. Bartkowiak K, Wieczorek M, Buck F, Harder S, Moldenhauer J, Effenberger KE, Pantel K, Peter-Katalinic J, Brandt BH. 2009. Two-dimensional differential gel electrophoresis of a cell line derived from a breast cancer micrometastasis revealed a stem/progenitor cell protein profile. J Proteome Res. 8:2004-2014. https://doi.org/10.1021/pr8009758
  2. Blackford JA Jr, Jones W, Dey RD, Castranova V. 1997. Comparison of inducible nitric oxide synthase gene expression and lung inflammation following intratracheal instillation of silica, coal, carbonyl iron, or titanium dioxide in rats. J Toxicol Environ Health. 51: 203-218. https://doi.org/10.1080/00984109708984022
  3. Borm PJ, Robbins D, Haubold S, Kuhlbusch T, Fissan H, Donaldson K, Schins R, Stone V, KreylingW, Lademann J, et al. 2006. The potential risks of nanomaterials: a review carried out the ECETOC. Part Fibre Toxicol. 3:11. https://doi.org/10.1186/1743-8977-3-11
  4. Boyd-Kimball D, Sultana R, Poon HF, Lynn BC, Casamenti F, Pepeu G, Klein JB, Butterfield DA. 2005. Proteomic identification of proteins specifically oxidized by intracerebral injection of amyloid beta-peptide (1-42) into rat brain: implications for Alzheimer's disease. Neuroscience. 132:313-324. https://doi.org/10.1016/j.neuroscience.2004.12.022
  5. Chen HW, Su SF, Chien CT, Lin WH, Yu SL, Chou CC, Chen JJ, Yang PC. 2006. Titanium dioxide nanoparticles induce emphysema-like lung injury in mice. FASEB J. 20:2393-2395. https://doi.org/10.1096/fj.06-6485fje
  6. Choi H, Stathatos E, Dionysiou DD. 2006. Sol-gel preparation of mesoporous photocatalytic $TiO_{2}$ films and $TiO_{2}$/$Al_{2}O_{3}$ composite membranes for environmental applications. Appl Catal B. 63:60-67. https://doi.org/10.1016/j.apcatb.2005.09.012
  7. Choi KM, Youn HS, Lee MY. 2009. Genomic and proteomic profiling of the cadmium cytotoxic response in human lung epithelial cells. Mol Cell Toxicol. 5:198-206.
  8. Dietschy JM, Turley SD. 2001. Cholesterol metabolism in the brain. Curr Opin Lipidol. 12:105-112. https://doi.org/10.1097/00041433-200104000-00003
  9. Ellman GL, Courtney KD, Andres V Jr, Feather-Stone RM. 1961. A new and rapid colorimetric determination of acetylcholinestrase activity. Biochem Pharmacol. 7:88-95. https://doi.org/10.1016/0006-2952(61)90145-9
  10. Gelman JS, Sironi J, Castro LM, Ferro ES, Fricker LD. 2010. Hemopressins and other hemoglobin-derived peptides in mouse brain: comparison between brain, blood, and heart peptidome and regulation in $Cpe^{fat/fat}$mice. J Neurochem. 113:871-880. https://doi.org/10.1111/j.1471-4159.2010.06653.x
  11. Haapasalo J, Nordfors K, Jarvela S, Bragge H, Rantala I, Parkkila AK, Haapasalo H, Parkkila S. 2007. Carbonic anhydrase II in the endothelium of glial tumors: a potential target for therapy. Neuro Oncol. 9:308-313. https://doi.org/10.1215/15228517-2007-001
  12. Ito J, Li H, Nagayasu Y, Kheirollah A, Yokoyama S. 2004. Apolipoprotein A-I induces translocation of protein kinase C$\alpha$ to a cytosolic lipid-protein particle in astrocytes. J Lipid Res. 45:2269-2276. https://doi.org/10.1194/jlr.M400222-JLR200
  13. Ito J, Kheirollah A, Nagayasu Y, Lu R, Kato K, Yokoyama S. 2006. Apolipoprotein A-I increases association of cytosolic cholesterol and caveolin-1 with microtubule cytoskeletons in rat astrocytes. J Neurochem. 97:1034-1043. https://doi.org/10.1111/j.1471-4159.2006.03805.x
  14. Jeon YM, Son BS, Lee MY. 2011. Proteomic identification of the differentially expressed proteins in human lung epithelial cells by airborne particulate matter. J Appl Toxicol. 31:45-52. https://doi.org/10.1002/jat.1566
  15. John GB, Shang Y, Li L, Renken C, Mannella CA, Selker JM, Rangell L, Bennett MJ, Zha J. 2005. The mitochondrial inner membrane protein mitofilin controls cristae morphology. Mol Biol Cell. 16:1543-1554. https://doi.org/10.1091/mbc.E04-08-0697
  16. Johnston HJ, Hutchison GR, Christensen FM, Peters S, Hankin S, Stone V. 2009. Identification of the mechanisms that drive the toxicity of $TiO_{2}$ particulates: the contribution of physicochemical characteristics. Part Fibre Toxicol. 6:33. https://doi.org/10.1186/1743-8977-6-33
  17. Kaida T, Kobayashi K., Adachi M, Suzuki F. 2004. Optical characteristics of titanium oxide interference film and the film laminated with oxides and their applications for cosmetics. J Cosmet Sci. 55:219-220.
  18. Kim JJ, Lee MY. 2009. Differential expression of cytosolic and nuclear proteins during s-nitrosoglutathione-induced cell death. BioChip J. 3:326-332.
  19. Kiss B, Biro T, Czifra G, Toth BI, Kertesz Z, Szikszai Z, Kiss AZ, Juhasz I, Zouboulis CC, Hunyadi J. 2008. Investigation of micronized titanium dioxide penetration in human skin xenografts and its effect on cellular functions of human skin-derived cells. Exp Dermatol. 17:659-667. https://doi.org/10.1111/j.1600-0625.2007.00683.x
  20. Lee MY, Shin HW. 2003. Cadmium-induced changes in antioxidant enzymes from the marine alga Nannochloropsis oculata. J Appl Phycol. 15:13-19. https://doi.org/10.1023/A:1022903602365
  21. Lin KM, Lin B, Lian IY, Mestril R, Scheffler IE, Dillmann WH. 2001. Combined and individual mitochondrial HSP60 and HSP10 expression in cardiac myocytes protects mitochondrial function and prevents apoptotic cell deaths induced by simulated ischemia-reoxygenation. Circulation. 103:1787-1792. https://doi.org/10.1161/01.CIR.103.13.1787
  22. Liu H, Ma L, Zhao J, Liu J, Yan J, Ruan J, Hong F. 2009. Biochemical toxicity of nano-anatase $TiO_{2}$ particles in mice. Biol Trace Elem Res. 129:170-180. https://doi.org/10.1007/s12011-008-8285-6
  23. Ma L, Liu J, Li N, Wang J, Duan Y, Yan J, Liu H, Wang H, Hong F. 2010. Oxidative stress in the brain of mice caused by translocated nanoparticulate $TiO_{2}$ delivered to the abdominal cavity. Biomaterials. 31:99-105. https://doi.org/10.1016/j.biomaterials.2009.09.028
  24. Myung JK, Gulesserian T, Fountoulakis M, Lubec G. 2003. Deranged hypothetical proteins Rik protein, Nit protein 2 and mitochondrial inner membrane protein, Mitofilin, in fetal Down syndrome brain. Cell Mol Biol. 49:739-746.
  25. Paglia DE, Valentine WN. 1967. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med. 70:158-169.
  26. Park EJ, Yi J, Chung KH, Ryu DY, Choi J, Park K. 2008. Oxidative stress and apoptosis induced by titanium dioxide nanoparticles in cultured BEAS-2B cells. Toxicol Lett. 180:222-229. https://doi.org/10.1016/j.toxlet.2008.06.869
  27. Park EJ, Yoon J, Choi K, Yi J, Park K. 2009. Induction of chronic inflammation in mice treated with titanium dioxide nanoparticles by intratracheal instillation. Toxicology. 260:37-46. https://doi.org/10.1016/j.tox.2009.03.005
  28. Park SK, Nam SW, Ryu JC, Ham JH, Lee MY. 2010. Proteomic analysis of rat liver proteins differentially induced by trichloethylene. BioChip J. 4:57-62. https://doi.org/10.1007/s13206-010-4109-9
  29. Roberts DJ, Waelbroeck M. 2004. G protein activation by G protein coupled receptors: ternary complex formation or catalyzed reaction? Biochem Pharmacol. 68:799-806. https://doi.org/10.1016/j.bcp.2004.05.044
  30. Shim JY, Om AS. 2008. Chlorella vulgaris has preventive effect on cadmium induced liver damage in rats. Mol Cell Toxicol. 4:138-143.
  31. Shim YJ, Lee MY. 2008. Identification of proteins in human follicular fluid by proteomic profiling. Mol Cell Toxicol. 4:253-259.
  32. Sohaebuddin SK, Thevenot PT, Baker D, Eaton JW, Tang L. 2010. Nanomaterial cytotoxicity is composition, size, and cell type dependent. Part Fibre Toxicol. 7:22. https://doi.org/10.1186/1743-8977-7-22
  33. Son JH, Kawamata H, Yoo MS, Kim DJ, Lee YK, Kim S, Dawson TM, Zhang H, Sulzer D, Yang L, et al. 2005. Neurotoxicity and behavioral deficits associated with septin 5 accumulation in dopaminergic neurons. J Neurochem. 94:1040-1053. https://doi.org/10.1111/j.1471-4159.2005.03257.x
  34. Son BS, Seong AR, Park SK, Kim WJ, Ryu JC, Lee MY. 2007. Toxicoproteomic analysis of differentially expressed proteins in rat liver by DEHP. Mol Cell Toxicol. 3:299-305.
  35. Trumpower BL. 1990. Cytochrome bc1 complexes of microorganisms. Microbiol Rev. 54:101-129.
  36. Tsang CW, Fedchyshyn M, Harrison J, Xie H, Xue J, Robinson PJ, Wang LY, Trimble WS. 2008. Superfluous role of mammalian septins 3 and 5 in neuronal development and synaptic transmission. Mol Cell Biol. 28:7012-7029. https://doi.org/10.1128/MCB.00035-08
  37. Wang JJ, Sanderson BJ, Wang H. 2007. Cyto- and genotoxicity of ultrafine $TiO_{2}$ particles in cultred human lymphoblastoid cells. Mutat Res. 628:99-106. https://doi.org/10.1016/j.mrgentox.2006.12.003
  38. Wang J, Liu Y, Jiao F, Lao F, Li W, Gu Y, Li Y, Ge C, Zhou G, Li B, et al. 2008. Time-dependent translocation and potential impairment on central nervous system by intranasally instilled $TiO_{2}$ nanoparticles. Toxicology. 254:82-90. https://doi.org/10.1016/j.tox.2008.09.014
  39. Warheit DB, Borm PJ, Hennes C, Lademann J. 2007. Testing strategies to establish the safety of nanomaterials: conclusion of an ECETOC workshop. Inhal Toxicol. 19:631-643. https://doi.org/10.1080/08958370701353080
  40. Xiao GG, Wang M, Li N, Loo JA, Nel AE. 2003. Use of proteomics to demonstrate a hierarchical oxidative stress response to diesel exhaust particle chemicals in a macrophage cell line. J Biol Chem. 278:50781-50790. https://doi.org/10.1074/jbc.M306423200
  41. Yamashita R, Fujiwara Y, Yuan X, Yasuda K, Kaburagi Y. 2005. 2-D LC-MS/MS analysis of secreted proteins from HepG2 cells: combination with various sample preparation methods before in-solution trypsin digestion. J Electrophoresis. 49:1-4. https://doi.org/10.2198/jelectroph.49.1
  42. Yoshiura K, Nakaoka T, Nishishita T, Sato K, Yamamoto A, Shimada S, Saida T, Kawakami Y, Takahashi TA, Fukuda H, et al. 2005. Carbonic anhydrase II is a tumor vessel endothelium-associated antigen targeted by dendritic cell therapy. Clin Cancer Res. 11:8201-8207. https://doi.org/10.1158/1078-0432.CCR-05-0816

피인용 문헌

  1. Neurotoxicity of nanoscale materials vol.22, pp.1, 2011, https://doi.org/10.1016/j.jfda.2014.01.012
  2. Toxicity of Nano-Titanium Dioxide (TiO2-NP) Through Various Routes of Exposure: a Review vol.172, pp.1, 2011, https://doi.org/10.1007/s12011-015-0550-x
  3. Experimental investigation of stability and transport of TiO2 nanoparticles in real soil columns vol.57, pp.54, 2016, https://doi.org/10.1080/19443994.2016.1163513
  4. Is Neurotoxicity of Metallic Nanoparticles the Cascades of Oxidative Stress? vol.11, pp.1, 2011, https://doi.org/10.1186/s11671-016-1508-4
  5. Nanoparticles as potential clinical therapeutic agents in Alzheimer’s disease: focus on selenium nanoparticles vol.10, pp.7, 2017, https://doi.org/10.1080/17512433.2017.1324781
  6. Role of omics techniques in the toxicity testing of nanoparticles vol.15, pp.None, 2011, https://doi.org/10.1186/s12951-017-0320-3
  7. Effects of Titanium Dioxide Nanoparticles Exposure on Human Health—a Review vol.193, pp.1, 2011, https://doi.org/10.1007/s12011-019-01706-6