The Effect of Pulsed Ultrasound on Skin Inflammatory Reaction Induced by Ultraviolet Irradiation

자외선 조사로 유발된 피부 염증성 반응에 맥동성 초음파가 미치는 영향

  • Lee, Jeong-Woo (Department of Physical Therapy, Kwangju Women's University) ;
  • Park, Su-Ji (Department of Physical Therapy, Kwangju Women's University) ;
  • Yoon, Se-Won (Department of Physical Therapy, Kwangju Women's University)
  • 이정우 (광주여자대학교 물리치료학과) ;
  • 박수지 (광주여자대학교 물리치료학과) ;
  • 윤세원 (광주여자대학교 물리치료학과)
  • Received : 2011.04.15
  • Accepted : 2011.08.03
  • Published : 2011.08.25

Abstract

Purpose: This study aims to examine the effects of pulsed ultrasound on skin inflammatory reaction induced by ultraviolet irradiation. Methods: Twenty subjects were selected for this study. Inflammatory reaction was induced by ultraviolet irradiation in two areas of the lumbar region of the subjects. Pulsed ultrasound (3 MHz) was applied to one of the two areas of inflammatory reaction at a pulse ratio of 1:4, intensity of $0.5W/cm^2$, once a day for 5 min, and pulsed ultrasound was not applied to the other area. Wound color (chromatic red), luminance (gray) and wound contraction (area) were measured using digital imaging processing method. Results: There was a significant difference in wound color (chromatic red) in the interaction between time and area. There was a significant difference in luminance in the interaction between time and area. There was a significant difference in wound contraction between the two areas. Conclusion: These results indicate that pulsed ultrasound increased the wound contraction rate and reduced the inflammatory reaction activity such as erythema induced by ultraviolet irradiation.

Keywords

References

  1. Clydesdale GJ, Dandie GW, Muller HK. Ultraviolet light induced injury:Immunological and inflammatory effects. Immunol Cell Biol. 2001;79(6):547-68. https://doi.org/10.1046/j.1440-1711.2001.01047.x
  2. Duthie MS, Kimber I, Norval M. The effects of ultraviolet radiation on the human immune system. Br J Dermatol. 1999;140(6):995-1009. https://doi.org/10.1046/j.1365-2133.1999.02898.x
  3. Svobodova A, Walterova D, Vostalove J. Ultraviolet light induced alteration to the skin. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2006;150(1):25-38. https://doi.org/10.5507/bp.2006.003
  4. Jung JH, Yoon JI, Ko WS et al. UVB induced minimal erythema dose (MED) and minimal melanogenic dose (MMD) in young adult koreans. Korean J Dermatol. 1994; 32(2):253-7.
  5. Kim JH, Park JK, Haw CR et al. The effects of multiple UVB exposures on proliferation and melanization in cultured human melanocyte. Korean J Dermatol. 1994;32(6):1035- 45.
  6. Yeo UC, Kim KH, Jo MK et al. Inflammatory skin response to ultraviolet radiation:ear swelling response in C57BL Mouse. Korean J Dermatol. 1989;27(6):650-4.
  7. Shono S, Imura M, Ota M et al. The relationship of skin color, uvb-induced erythema, and melanogenesis. J invest Dermatol. 1985;84(4):265-7. https://doi.org/10.1111/1523-1747.ep12265342
  8. Woodley DT, O'Keefe EJ, Prunieras M. Cutaneous wound healing: a model for cell-matrix interactions. J Am Acad Dermatol. 1985;12(2 Pt 2):420-33. https://doi.org/10.1016/S0190-9622(85)80005-0
  9. Hopkins JT, McLoda TA, Seegmiller JG et al. Low-level laser therapy facilitates superficial wound healing in humans: A triple-blind, sham-controlled study. J Athl Train. 2004; 39(3):223-9.
  10. Oh HJ, Kim JW, Kim MS et al. The effect of microcurrent stimulation on histological structure of wound in rat. J Kor Soc Phys Ther. 2008;20(1):67-73 https://doi.org/10.1589/jpts.20.67
  11. Lowe AS, Walker MD, Cowan R et al. Therapeutic ultrasound and wound closer: lack of healing effect on x-ray irradiated wound in murine skin. Arch Phys Med Rehabil. 2001;82(11): 1507-11. https://doi.org/10.1053/apmr.2001.25083
  12. Kim SM, Lee MP, Choi, BC et al. A study of ultrasound rehabilitation therapy:Physiological effects by change of ultrasound intensity. Journal of biomedical engineering research. 2008;29(1):40-5.
  13. 13. Min SG, Kim KY, Oh MH. The effects of wound healing of pulsed ultrasound in diabetic induced rats. J Kor Acad Clin Elec. 2003;1:89-100.
  14. Nyborg WL. Ultrasonic microstreaming and related phenomena. Br J Cancer Suppl. 1982;5:156-60.
  15. Duarte LR. The stimulation of bone growth by ultrasound. Arch Orthop Trauma Surg. 1983;101(3):153-9. https://doi.org/10.1007/BF00436764
  16. Klug W, Franke WG, Knoch HG. Scintigraphic control of bone-fracutre healing under ultrasonic stimulation: An animal experimental study. Eur J Nucl Med. 1986;11(12): 494-7.
  17. Hadjiarhyrou M, McLeod K, Ryaby JP et al. Enhancement of fracutre healing by low intensity ultrasound. Clin Orthop Relat Res. 1998;355:S216-29.
  18. Ko HY, Kim KS.Tissue temperature change according to ultrasound application techniques. J Korean Acad Rehab Med. 1993;17(1):76-80.
  19. Dinno MA, Dyson M, Young SR et al. The significance of memebrane changes in the safe and effectvie use of therapeutic and diagnostic ultrasound. Phys Med Biol. 1989; 34(11):1543-52. https://doi.org/10.1088/0031-9155/34/11/003
  20. Dyson M. Non-thermal cellular effects of ultrasound. Br J Cancer Suppl. 1982;5:165-71.
  21. Mortimer AJ, Dyson M. The effect of therapeutic ultrasound on calcium uptake in fibroblasts. Ultrasound Med Biol. 1988;14(6):499-506. https://doi.org/10.1016/0301-5629(88)90111-1
  22. Altomare M, Nascimento AP, Romana-Souza B et al. Ultraound accelerates healing of normal wounds but not of ischemic ones. Wound Repair Regen. 2009;17(6):825-31. https://doi.org/10.1111/j.1524-475X.2009.00542.x
  23. Ikail H, Tamura T, Watanabe T et al. Low-intensity pulsed ultrasound accelerates periodontal wound healing after flap surgery. J Periodontal Res. 2008;43(2):212-6. https://doi.org/10.1111/j.1600-0765.2007.01016.x
  24. Della Rocca GJ. The science of ultrasound therapy for fracture healing. Indian J orthop. 2009;43(2):121-6. https://doi.org/10.4103/0019-5413.50845
  25. Pounder NM, Harrison AJ. Low intensity pulsed ultrasound for fracutre healing: A review of the clinical evidence and the associated biological mechanism of action. Ultrasonics. 2008;48(4):330-8. https://doi.org/10.1016/j.ultras.2008.02.005
  26. Min SG, Chun JS, Young JH et al. The effects of pulsed ultrasound on the bone healing process in fracture model of diabetes mellitus rat. J Kor Soc Phys Ther. 2006;18(2): 47-58.
  27. Jang C, Bae SS, Kim HJ. The effects of ultrasound treatment on the angle of cervical vertebrae movement of VDT syndrome patient. J Kor Soc Phys Ther. 2003;15(3):277-94.
  28. Warden SJ, Metcalf BR, Kiss ZS et al. Low-intensity pulsed ultrasound for chronic patellar tendinopathy: A randomized, double-blind, placebo-controlled trial. Rheumatology (Oxford). 2008;47(4):467-71.
  29. Lee SH, Pi ER, Jung HN et al. The effects of ESWT and US on muscle power, pain and muscle tension at trigger point of the sapular inter angle. J Kor Acad Clin Elec. 2009;7(1):29-34.
  30. Lee JH, Park CE, Park RJ. Electrical Stimulation Induces the Collagen Deposition and TGF-$\beta$1 RNA Expression in Skin Wound of Rat. J Kor Soc Phys Ther. 2010;22(3):87-92.
  31. Lee HM, Chae YW. Influence of Microcurrent Therapy in Interleukin-1 Expression in Rhueumatoid Arthritis Rats. J Kor Soc Phys Ther. 2009;21(2):103-8.
  32. Ha MS, Park RJ. The Effect of Electroacupuncture on Concentration and Activity of CK, ALP, and IgG in Serum and Central Nervous System of Rats. J Kor Soc Phys Ther. 2010;22(2):47-54.
  33. Han DU, Nam HC, Park RJ. Phototherapy 5th edition. Seoul, Jeongdammidia, 2008:80-1.
  34. Ham YU. Phototherapy 4th edition. Seoul, Hyeonmunsa, 1995:98.
  35. Hansen GL, Sparrow EM, Kokate JY et al. Wound status evaluation using color image processing. IEEE Trans Med Imaging. 1997;16(1):78-86. https://doi.org/10.1109/42.552057
  36. Rafasekaran NS, Nithya M, Rose C et al. The effect of finger millet feeding on the early responses during the process of wound healing in diabetic rats. Biochim Biophys Acta. 2004;1689(3):190-201. https://doi.org/10.1016/j.bbadis.2004.03.004
  37. Bon FX, Briand E, Guichard S et al. Quantitative and kinetic evolution of wound healing through image analysis. IEEE Trans Med Imaging. 2000;19(7):767-72. https://doi.org/10.1109/42.875206
  38. Gostishchev VK, Baichorov EKh, Berchenko GN. Effect of low-frequency ultrasound on the course of the wound process. Vestn Khir Im II Grek. 1984;133(10):110-3.
  39. Kim JS, Park SW, Choi TH et al. The evaluation of relevant factors influencing skin graft changes in color over time. Dermatol Surg. 2008;34(1):32-9.
  40. Byl NN, McKenzie AL, West JM et al. Low-dose ultrasound effects on wound healing: A controlled study with yucatan pigs. Arch Phys Med Rehabil. 1992;73(7):656-64.