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Effects of lipopolysaccharide and CpG-DNA on burn-induced skin injury

  • Park, Byoung-Kwon (Department of Microbiology, College of Medicine, Hallym University) ;
  • Kim, Dong-Bum (Department of Microbiology, College of Medicine, Hallym University) ;
  • Cho, Sun-Hee (Department of Microbiology, College of Medicine, Hallym University) ;
  • Seo, Jae-Nam (Department of Pathology, College of Medicine, Hallym University) ;
  • Park, Jae-Bong (Department of Biochemistry, College of Medicine, Hallym University) ;
  • Kim, Yong-Sun (Department of Microbiology, College of Medicine, Hallym University) ;
  • Choi, Ihn-Geun (Department of Neuropsychiatry, Hallym University, Han-Gang Sacred Heart Hospital) ;
  • Kwon, Hyeok-Yil (Department of Physiology, College of Medicine, Hallym University) ;
  • Lee, Young-Hee (Department of Biochemistry, College of Natural Sciences, Chungbuk National University) ;
  • Kwon, Hyung-Joo (Department of Microbiology, College of Medicine, Hallym University)
  • Received : 2011.01.17
  • Accepted : 2011.02.09
  • Published : 2011.04.30
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Abstract

Destruction of the skin barrier by thermal injury induces microbial invasion, which can lead to the development of systemic infection and septic shock. Microbial pathogens possess pathogen-associated molecular patterns (PAMPs), which are recognized by conserved receptors. To understand the role of PAMPs in thermal injury-induced mice, LPS or CpG-DNA were topically applied to dorsal skin after thermal injury. We observed an increase in the number of inflammatory cell infiltrates as well as thickening in the dermis upon treatment with LPS or CpG-DNA. We also found that expression of IL-$1{\beta}$, MIP-2, and RANTES induced by thermal injury was enhanced by LPS or CpG-DNA. In addition, the proportions of $CD4^+$ and $CD^8+$ T cells in the spleen and lymph nodes were altered by LPS or CpG-DNA. These results provide important information concerning PAMPs-induced inflammation upon thermal injury and provide a basis for studying the role of PAMPs in thermal injury-induced complications.

Keywords

References

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