Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Analytical Method Validation of Quercetin in Changnyeong Onion Extract as a Functional Ingredient for Functional Health Food

건강기능식품 기능성원료로서 창녕양파추출액의 지표성분 Quercetin 분석법

  • Jeon, Seon-Young (Dept. of Food and Nutrition, Changwon National University) ;
  • Jeong, Eun-Jeong (Dept. of Food and Nutrition, Changwon National University) ;
  • Baek, Jeong-Hwa (Dept. of Food and Nutrition, Changwon National University) ;
  • Cha, Yong-Jun (Dept. of Food and Nutrition, Changwon National University)
  • 전선영 (창원대학교 식품영양학과) ;
  • 정은정 (창원대학교 식품영양학과) ;
  • 백정화 (창원대학교 식품영양학과) ;
  • 차용준 (창원대학교 식품영양학과)
  • Received : 2011.02.07
  • Accepted : 2011.03.02
  • Published : 2011.04.30
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Abstract

Validation of quercetin as a marker compound in the standardization of Changnyeong onion extract developed for functional health food was attempted by analytical method. The specificity was satisfied with retention time and photo diode array (PDA) spectrum by analysis of quercetin using HPLC and comparison with standard compound. It showed a high linearity in the calibration curve as coefficient of correlation ($R^2$) of 0.9986, and the limit of detection (LOD) and limit of quantitation (LOQ) were 0.2 mg/L and 0.5 mg/L, respectively. Recovery rate test with quercetin concentration of 0.05, 0.075 and 0.1 mg/mL was revealed in the high range of 82.36~95.26%, 82.70~98.24% and 87.91~95.11%, respectively. The intra-day and inter-day precision in quercetin for Changnyeong onion extracts was 0.10~3.28% and 0.96~5.79%, respectively. Therefore, application of quercetin was validated in analytical method as a marker compound in Changnyeong onion extracts.

개별인정형 건강기능식품 기능성 원료로 개발한 창녕양파추출액의 표준화를 위해 지표성분으로 quercetin을 설정하였으며, HPLC를 이용하여 지표성분 quercetin의 분석법을 확립하며 그에 따른 유효성 검정을 실시하고자 하였다. 유효성 검정 결과, 본 시험법에서 표준용액의 피크유지시간과 창녕양파추출액의 피크유지시간이 일치하였으며 동일한 spectrum을 나타내어 특이성을 확인하였다. 검량선의 상관계수($R^2$)는 0.9986로 높은 직선성을 보여 분석에 적합함을 알 수 있었으며, 검출한계는 0.2 mg/L, 정량한계는 0.5 mg/L 로 설정되었다. Quercetin의 회수율은 0.05 mg/mL에서는 82.36~95.26%, 0.075 mg/mL는 82.70~98.24%, 0.1 mg/mL은 87.91~95.11%의 범위의 회수율을 보였으며, intra-day에서의 정밀도(RSD)는 0.10~3.28%, inter-day에서는 0.96~5.79%의 정밀도를 나타내어 창녕양파추출액의 지표성분인 quercetin의 분석법은 적합한 시험법임이 검증되었다.

Keywords

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