Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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Hydrophillic Interaction Chromatography-tandem Mass Spectrometry Method for Identification and Quantitation of 5-MeO-DIPT and its Metabolites in Rat Urine

  • Kim, Yoon (Doping Control Center, Korea Institute of Science and Technology) ;
  • Kim, Un-Yong (Doping Control Center, Korea Institute of Science and Technology) ;
  • In, Moon-Kyo (Drug Analysis Laboratory, Supreme Prosecutors' Office) ;
  • Lee, Jae-Ick (Doping Control Center, Korea Institute of Science and Technology) ;
  • Kwon, Oh-Seung (Doping Control Center, Korea Institute of Science and Technology) ;
  • Yoo, Hye-Hyun (Doping Control Center, Korea Institute of Science and Technology)
  • Received : 2010.10.02
  • Accepted : 2011.01.29
  • Published : 2011.04.20
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Abstract

5-Methoxy-N,N-diisopropyltryptamine (5-MeO-DIPT), a psychoactive tryptamine derivative, is a hallucinogenic drug of abuse. In this study, 5-OH-DIPT and its metabolites were identified and the quantitative method was developed and validated by using hydrophilic interaction chromatography-tandem mass spectrometry (HILICMS/MS). Chromatographic separation was achieved on an Atlantis HILIC silica column ($5{\mu}m$, $100{\times}2.1\;mm$). The metabolites of 5-MeO-DIPT in rat urine were characterized via Q1 scanning and product ion scanning. As a consequence, 5-MeO-IPT, 5-OH-DIPT, 6-OH-5-MeO-DIPT and their glucuronide conjugates were detected and identified as the metabolites of 5-MeO-DIPT. Subsequently, a quantitative method for 5-MeO-DIPT and its major metabolites, 5-MeO-IPT and 5-OH-DIPT, was developed in multiple reactions monitoring (MRM) mode. The calibration curves for all analytes evidenced good linearity over the concentration range of 1-1000 ng/mL with linear correlation co-efficients ($r^2$) in excess of 0.99. The intra- and inter-day accuracy and precision were 92.2-110.2% and 1.5-9.9%, respectively.

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References

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