Biomolecules & Therapeutics

  • 제19권2호
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  • Pages.195-200
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  • 2011
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  • 1976-9148(pISSN)
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  • 2005-4483(eISSN)
한국응용약물학회 (The Korean Society of Applied Pharmacology)
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Cytoprotective Effects of Docosyl Cafferate against tBHP-Induced Oxidative Stress in SH-SY5Y Human Neuroblastoma Cells

  • Choi, Yong-Jun (Department of Pharmacology, College of Medicine, Kangwon National University) ;
  • Kwak, Eun-Bee (Department of Pharmacology, College of Medicine, Kangwon National University) ;
  • Lee, Jae-Won (Department of Pharmacology, College of Medicine, Kangwon National University) ;
  • Lee, Yong-Suk (Department of Anesthesiology, College of Medicine, Kangwon National University) ;
  • Cheong, Il-Young (Department of Anesthesiology, College of Medicine, Kangwon National University) ;
  • Lee, Hee-Jae (Department of Pharmacology, College of Medicine, Kangwon National University) ;
  • Kim, Sung-Soo (Department of Pharmacology, College of Medicine, Kangwon National University) ;
  • Kim, Myong-Jo (Division of Bio-resources Technology, Kangwon National University) ;
  • Kwon, Yong-Soo (College of Pharmacy, Kangwon National University) ;
  • Chun, Wan-Joo (Department of Pharmacology, College of Medicine, Kangwon National University)
  • 투고 : 2011.02.01
  • 심사 : 2011.03.03
  • 발행 : 2011.04.30
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초록

Neuronal cell death is a common characteristic feature of a variety of neurodegenerative disorders including Alzheimer's disease and Parkinson's disease. However, there have been no effective drugs to successfully prevent neuronal death in those diseases. In the present study, docosyl cafferate (DC), a derivative of caffeic acid, was isolated from Rhus verniciflua and its protective effects on tBHP-induced neuronal cell death were examined in SH-SY5Y human neuroblastoma cells. Pretreatment of DC significantly attenuated tBHP-induced neuronal cell death in a concentration-dependent manner. DC also significantly suppressed tBHP-induced caspase-3 activation. In addition, DC restored tBHP-induced depletion of intracellular Bcl-2, an anti-apoptotic member of the Bcl-2 family. Furthermore, DC significantly suppressed tBHP-induced degradation of IKB, which retains $NF-{\kappa}B$ in the cytoplasm, resulting in the suppression of nuclear translocation of $NF-{\kappa}B$ and its subsequent activation. Taken together, the results clearly demonstrate that DC exerts its neuroprotective activity against tBHP-induced oxidative stress through the suppression of nuclear translocation of $NF-{\kappa}B$.

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참고문헌

  1. Amoroso, S., Gioielli, A., Cataldi, M., Di Renzo, G. and Annunziato, L. (1999) In the neuronal cell line SH-SY5Y, oxidative stress-induced free radical overproduction causes cell death without any participation of intracellular $Ca^{2+}$ increase. Biochim. Biophys. Acta. 1452, 151-160. https://doi.org/10.1016/S0167-4889(99)00110-X
  2. Ashikawa, K., Shishodia, S., Fokt, I., Priebe, W. and Aggarwal, B. B. (2004) Evidence that activation of nuclear factor-kappaB is essential for the cytotoxic effects of doxorubicin and its analogues. Biochem. Pharmacol. 67, 353-364. https://doi.org/10.1016/j.bcp.2003.08.039
  3. Barger, S. W., Horster, D., Furukawa, K., Goodman, Y., Krieglstein, J. and Mattson, M. P. (1995) Tumor necrosis factors alpha and beta protect neurons against amyloid beta-peptide toxicity: evidence for involvement of a kappa B-binding factor and attenuation of peroxide and $Ca^{2+}$ accumulation. Proc. Natl. Acad. Sci. U S A 92, 9328-9332. https://doi.org/10.1073/pnas.92.20.9328
  4. Bian, X., McAllister-Lucas, L. M., Shao, F., Schumacher, K. R., Feng, Z., Porter, A. G., Castle, V. P. and Opipari, A. W., Jr. (2001) NF-kappa B activation mediates doxorubicin-induced cell death in N-type neuroblastoma cells. J. Biol. Chem. 276, 48921-48929. https://doi.org/10.1074/jbc.M108674200
  5. Byun, J. S., Han, Y. H., Hong, S. J., Hwang, S. M., Kwon, Y. S., Lee, H. J., Kim, S. S., Kim, M. J. and Chun, W. (2010) Bark constituents from mushroom-detoxified Rhus verniciflua suppress kainic acid-induced neuronal cell death in mouse hippocampus. Korean J. Physiol. Pharmacol. 14, 279-283. https://doi.org/10.4196/kjpp.2010.14.5.279
  6. Chandra, J., Samali, A. and Orrenius, S. (2000) Triggering and modulation of apoptosis by oxidative stress. Free Radic. Biol. Med. 29, 323-333. https://doi.org/10.1016/S0891-5849(00)00302-6
  7. Cho, I. H., Hong, J., Suh, E. C., Kim, J. H., Lee, H., Lee, J. E., Lee, S., Kim, C. H., Kim, D. W., Jo, E. K., Lee, K. E., Karin, M. and Lee, S. J. (2008) Role of microglial IKKbeta in kainic acid-induced hippocampal neuronal cell death. Brain 131, 3019-3033. https://doi.org/10.1093/brain/awn230
  8. Clemens, J. A., Stephenson, D. T., Smalstig, E. B., Dixon, E. P. and Little, S. P. (1997) Global ischemia activates nuclear factor-kappa B in forebrain neurons of rats. Stroke 28, 1073-1080; discussion 1080-1071. https://doi.org/10.1161/01.STR.28.5.1073
  9. Deng, M., Zhao, J. Y., Tu, P. F., Jiang, Y., Li, Z. B. and Wang, Y. H. (2004) Echinacoside rescues the SHSY5Y neuronal cells from TNFalpha-induced apoptosis. Eur. J. Pharmacol. 505, 11-18. https://doi.org/10.1016/j.ejphar.2004.09.059
  10. Friedlander, R. M. (2003) Apoptosis and caspases in neurodegenerative diseases. N. Engl. J. Med. 348, 1365-1375. https://doi.org/10.1056/NEJMra022366
  11. Goodman, Y. and Mattson, M. P. (1996) Ceramide protects hippocampal neurons against excitotoxic and oxidative insults, and amyloid beta-peptide toxicity. J. Neurochem. 66, 869-872.
  12. Halliwell, B. (1989) Free radicals, reactive oxygen species and human disease: a critical evaluation with special reference to atherosclerosis. Br. J. Exp. Pathol. 70, 737-757.
  13. Hyslop, P. A., Zhang, Z., Pearson, D. V. and Phebus, L. A. (1995) Measurement of striatal H2O2 by microdialysis following global forebrain ischemia and reperfusion in the rat: correlation with the cytotoxic potential of H2O2 in vitro. Brain Res. 671, 181-186. https://doi.org/10.1016/0006-8993(94)01291-O
  14. Iinuma, M., Tanaka, T., Mizuno, M. and Lang, F. A. (1992) Two flavoanones from roots of Sophora leachiana. Phytochemistry 31, 721-723. https://doi.org/10.1016/0031-9422(92)90075-2
  15. Jeon, W. K., Lee, J. H., Kim, H. K., Lee, A. Y., Lee, S. O., Kim, Y. S., Ryu, S. Y., Kim, S. Y., Lee, Y. J. and Ko, B. S. (2006) Anti-platelet effects of bioactive compounds isolated from the bark of Rhus verniciflua Stokes. J. Ethnopharmacol. 106, 62-69. https://doi.org/10.1016/j.jep.2005.12.015
  16. Jeong, J. S. and Park, J. W. (2008) Carcinostatic effect of allergen removed Rhus verniciflua stokes based traditional Korean medicine on a patient with lung adenocarcinoma; single case report. OPEM 7, 573-578. https://doi.org/10.3742/OPEM.2008.7.5.573
  17. Jiang, W. L., Tian, J. W., Fu, F. H., Zhu, H. B. and Hou, J. (2010) Neuroprotective efficacy and therapeutic window of Forsythoside B: in a rat model of cerebral ischemia and reperfusion injury. Eur. J. Pharmacol. 640, 75-81. https://doi.org/10.1016/j.ejphar.2010.04.055
  18. Kang, S. and Kim, C. (1987) Studies on the Korean Indigenous Plants. Isolation of 1-eicosanoyl cafferate from Echinosophora koreensis. Arch. Pharm. Res. 10, 67-68. https://doi.org/10.1007/BF02855623
  19. Karin, M., Takahashi, T., Kapahi, P., Delhase, M., Chen, Y., Makris, C., Rothwarf, D., Baud, V., Natoli, G., Guido, F. and Li, N. (2001) Oxidative stress and gene expression: the AP-1 and NF-kappaB connections. Biofactors 15, 87-89. https://doi.org/10.1002/biof.5520150207
  20. Kim, M. and Choi, W. (2002) Anticancer and antioxidant activity of allergen-removed extract in Rhus verniciflua stokes. Korean J. Med. Crop Sci 10, 288-293.
  21. Kim, M., Choi, Y. H., Kim W. G. and Kwak S. S. (1997) Antioxidative activity of urushiol derivatives from the sap of lacquer tree (Rhus verniciflua Stokes). Korean J. Plant. Res. 10, 227-230.
  22. Kim, S. R. and Kim, Y. C. (2000) Neuroprotective phenylpropanoid esters of rhamnose isolated from roots of Scrophularia buergeriana. Phytochemistry 54, 503-509. https://doi.org/10.1016/S0031-9422(00)00110-2
  23. Kim, Y. (2010) Neuroprotective phenolics in medicinal plants. Arch. Pharm. Res. 33, 1611-1632. https://doi.org/10.1007/s12272-010-1011-x
  24. Komatsu, M., Tomimori, T., Hatayama, K. and Makiguchi, Y. (1970) Studies on the constituents of Sophora species. 3. Constituents of the root of Sophora subprostrata Chun et T. Chen. Yakugaku Zasshi 90, 459-462.
  25. Lapchak, P. A. (2007) The phenylpropanoid micronutrient chlorogenic acid improves clinical rating scores in rabbits following multiple infarct ischemic strokes: synergism with tissue plasminogen activator. Exp. Neurol. 205, 407-413. https://doi.org/10.1016/j.expneurol.2007.02.017
  26. Li, Q. and Verma, I. M. (2002) NF-kappaB regulation in the immune system. Nat. Rev. Immunol. 2, 725-734. https://doi.org/10.1038/nri910
  27. Li, Y. Y., Lu, J. H., Li, Q., Zhao, Y. Y. and Pu, X. P. (2008) Pedicularioside A from Buddleia lindleyana inhibits cell death induced by 1-methyl-4-phenylpyridinium ions (MPP+) in primary cultures of rat mesencephalic neurons. Eur. J. Pharmacol. 579, 134-140. https://doi.org/10.1016/j.ejphar.2007.10.052
  28. Liu, B., Gao, H. M., Wang, J. Y., Jeohn, G. H., Cooper, C. L. and Hong, J. S. (2002) Role of nitric oxide in inflammation-mediated neurodegeneration. Ann. N Y Acad. Sci. 962, 318-331. https://doi.org/10.1111/j.1749-6632.2002.tb04077.x
  29. Liu, H., Miller, E., van de Water, B. and Stevens, J. L. (1998) Endoplasmic reticulum stress proteins block oxidant-induced $Ca^{2+}$ increases and cell death. J. Biol. Chem. 273, 12858-12862. https://doi.org/10.1074/jbc.273.21.12858
  30. Mattson, M. P., Goodman, Y., Luo, H., Fu, W. and Furukawa, K. (1997) Activation of NF-kappaB protects hippocampal neurons against oxidative stress-induced apoptosis: evidence for induction of manganese superoxide dismutase and suppression of peroxynitrite production and protein tyrosine nitration. J. Neurosci. Res. 49, 681-697. https://doi.org/10.1002/(SICI)1097-4547(19970915)49:6<681::AID-JNR3>3.0.CO;2-3
  31. Nohl, H., Gille, L., Schonheit, K. and Liu, Y. (1996) Conditions allowing redox-cycling ubisemiquinone in mitochondria to establish a direct redox couple with molecular oxygen. Free Radic. Biol. Med. 20, 207-213. https://doi.org/10.1016/0891-5849(95)02038-1
  32. Park, K. Y., Jung, G. O., Lee, K. T., Choi, J., Choi, M. Y., Kim, G. T., Jung, H. J. and Park, H. J. (2004) Antimutagenic activity of flavonoids from the heartwood of Rhus verniciflua. J. Ethnopharmacol. 90, 73-79. https://doi.org/10.1016/j.jep.2003.09.043
  33. Romano, M. F., Avellino, R., Petrella, A., Bisogni, R., Romano, S. and Venuta, S. (2004) Rapamycin inhibits doxorubicin-induced NF-kappaB/Rel nuclear activity and enhances the apoptosis of melanoma cells. Eur. J. Cancer 40, 2829-2836. https://doi.org/10.1016/j.ejca.2004.08.017
  34. Simonian, N. A. and Coyle, J. T. (1996) Oxidative stress in neurodegenerative diseases. Annu. Rev. Pharmacol. Toxicol. 36, 83-106. https://doi.org/10.1146/annurev.pa.36.040196.000503
  35. Sul, D., Kim, H. S., Lee, D., Joo, S. S., Hwang, K. W. and Park, S. Y. (2009) Protective effect of caffeic acid against beta-amyloid-induced neurotoxicity by the inhibition of calcium influx and tau phosphorylation. Life Sci. 84, 257-262. https://doi.org/10.1016/j.lfs.2008.12.001
  36. Tsai, S. K., Lin, M. J., Liao, P. H., Yang, C. Y., Lin, S. M., Liu, S. M., Lin, R. H., Chih, C. L. and Huang, S. S. (2006) Caffeic acid phenethyl ester ameliorates cerebral infarction in rats subjected to focal cerebral ischemia. Life Sci. 78, 2758-2762. https://doi.org/10.1016/j.lfs.2005.10.017
  37. Wang, S., Kotamraju, S., Konorev, E., Kalivendi, S., Joseph, J. and Kalyanaraman, B. (2002) Activation of nuclear factor-kappaB during doxorubicin-induced apoptosis in endothelial cells and myocytes is pro-apoptotic: the role of hydrogen peroxide. Biochem. J. 367, 729-740. https://doi.org/10.1042/BJ20020752
  38. Wei, X., Ma, Z., Fontanilla, C. V., Zhao, L., Xu, Z. C., Taggliabraci, V., Johnstone, B. H., Dodel, R. C., Farlow, M. R. and Du, Y. (2008) Caffeic acid phenethyl ester prevents cerebellar granule neurons (CGNs) against glutamate-induced neurotoxicity. Neuroscience 155, 1098-1105. https://doi.org/10.1016/j.neuroscience.2008.06.056

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