Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Effects of Dietary Inclusion of Various Additives on Growth Performance, Hematological Parameters, Fatty Acid Composition, Gene Expression and Histopathological Changes in Juvenile Olive Flounder Paralichthys olivaceus

배합사료의 다양한 첨가제가 넙치(Paralichthys olivaceus) 치어의 성장, 혈액 성상, 지방산 조성, 유전자 발현 및 조직 성상에 미치는 영향

  • Kim, Kyoung-Duck (Aquafeed Research Center, National Fisheries Research and Development Institute) ;
  • Seo, Joo-Young (Gangwon-Do Deep Sea Water & Fishery Resource Center) ;
  • Hong, Su-Hee (Department of Marine Biotechnology, Gangneung-Wonju National University) ;
  • Kim, Jeong-Ho (Department of Marine Resources Development, Gangneung-Wonju National University) ;
  • Byun, Hee-Guk (Department of Marine Biotechnology, Gangneung-Wonju National University) ;
  • Kim, Kang-Woong (Aquafeed Research Center, National Fisheries Research and Development Institute) ;
  • Son, Maeng-Hyun (Aquafeed Research Center, National Fisheries Research and Development Institute) ;
  • Lee, Sang-Min (Department of Marine Biotechnology, Gangneung-Wonju National University)
  • 김경덕 (국립수산과학원 사료연구센터) ;
  • 서주영 (강원도 해양심층수 수산자원센터) ;
  • 홍수희 (강릉원주대학교 해양생물공학과) ;
  • 김정호 (강릉원주대학교 해양자원육성학과) ;
  • 변희국 (강릉원주대학교 해양생물공학과) ;
  • 김강웅 (국립수산과학원 사료연구센터) ;
  • 손맹현 (국립수산과학원 사료연구센터) ;
  • 이상민 (강릉원주대학교 해양생물공학과)
  • Received : 2011.01.12
  • Accepted : 2011.04.06
  • Published : 2011.04.30
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Abstract

This feeding experiment was conducted to investigate the effects of dietary inclusion of various additives on growth performance, hematological parameters, fatty acid composition, gene expression and histopathological changes in juvenile olive flounder (Paralichthys olivaceus). Eleven isonitrogenous (49% crude protein) and isolipidic (10% crude lipid) experimental diets were formulated: no additives (Con); 5% kelp meal (Ke); 10% krill meal (Kr); 1% garlic powder (Ga); 1% citrus meal (Ci); 3% onion powder (On); 1% ginger powder (Gi); 1% mugwort powder (Mu); 1% licorice powder (Li); 1% wasabi powder (Wa); and a mixture (Mix) of these additives. Three replicate groups of juvenile flounder (average weight of 8.5 g) were fed one of the experimental diets to visual satiety twice a day for 15 weeks. The dietary inclusion of additives did not affect survival, weight gain, specific growth rate feed efficiency, daily feed intake, daily protein intake, protein efficiency ratio, hepatosomatic index and visceralsomatic index of the fish. Plasma triglyceride levels were significantly lower in fish fed the Ke, Ga, On, Gi, Mu, Li, and Mix diets than in fish fed the control diet. Plasma glucose, glutamic oxaloacetic transaminase and total cholesterol did not differ among dietary treatments. No significant difference was observed in fatty acid composition and lipid content of the dorsal muscle in fish fed the experimental diets. Myosin gene expression did not differ significantly among treatments after 5 weeks but was significantly lower in fish fed the Kr, Ci, Li, and Mix diets than in control group after 15 weeks. Histopathological analysis showed mild gill hyperplasia and mild necrosis of liver parenchymal cells in several individuals of each experimental group. These conditions were also observed in the control group and were not thought to be related to the inclusion of feed additives. The present findings indicate that the dietary inclusion of additives did not affect growth performance, fatty acid composition, gene expression, and histopathological changes in juvenile flounder. However, plasma triglyceride content may be reduced by supplementation with 5% kelp meal, 3% onion powder, 1% garlic powder, 1% ginger powder, 1% mugwort powder, and the additive mixture.

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References

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