Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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Development of an ISSR-Derived SCAR Marker in Korean Ginseng Cultivars (Panax ginseng C. A. Meyer)

  • Lee, Jei-Wan (Division of Forest Genetic Resources, Korea Forest Research Institute) ;
  • Kim, Young-Chang (National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Jo, Ick-Hyun (National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Seo, A-Yeon (National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Lee, Jeong-Hoon (National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Kim, Ok-Tae (National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Hyun, Dong-Yun (National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Cha, Seon-Woo (National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Bang, Kyong-Hwan (National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Cho, Joon-Hyeong (Department of Biological and Environmental Science, Dongguk University)
  • Received : 2010.10.06
  • Accepted : 2011.01.17
  • Published : 2011.03.29
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Abstract

Recently, new ginseng cultivars having superior agricultural traits have been developed in Korea. For newly developed plant cultivars, the identification of distinctiveness is very important factors not only in plant cultivar management but also in breeding programs. Thus, eighty-five inter simple sequence repeat (ISSR) primers were applied to detect polymorphisms among six major Korean ginseng cultivars and two foreign ginsengs. A total of 197 polymorphic bands with an average 5.8 polymorphic bands and 2.9 banding patterns per assay unit across six Korean ginseng cultivars and foreign ginsengs from 236 amplified ISSR loci with an average 6.9 loci per assay unit were generated by 34 out of 85 ISSR primers. Three species of Panax ginseng including the Korean ginseng cultivars, P. quinquefolius, and P. notoginseng, could be readily discriminated using most tested primers. UBC-821, UBC-868, and UBC-878 generated polymorphic bands among the six Korean ginseng cultivars, and could distinguish them from foreign ginsengs. Sequence characterized amplified region (SCAR) marker system was introduced in order to increase the reproducibility of the polymorphism. One SCAR marker, PgI821C650, was successfully converted from the randomly amplified polymorphism by UBC-821. It showed the expected dominant polymorphism among ginseng samples. In addition, the specific polymorphism for Sunwon was generated by treating Taq I restriction enzyme to polymerase chain reaction products of PgI821C650. These results will serve as useful DNA markers for identification of Korean ginseng, especially Sunwon cultivar, seed management, and molecular breeding program supplemented with marker-assisted selection.

Keywords

References

  1. Wen J, Zimmer EA. Phylogeny and biogeography of Panax L. (the ginseng genus, araliaceae): inferences from ITS sequences of nuclear ribosomal DNA. Mol Phylogenet Evol 1996;6:167-177. https://doi.org/10.1006/mpev.1996.0069
  2. Liu J, Wang S, Liu H, Yang L, Nan G. Stimulatory effect of saponin from Panax ginseng on immune function of lymphocytes in the elderly. Mech Ageing Dev 1995;83:43-53. https://doi.org/10.1016/0047-6374(95)01618-A
  3. Shin HR, Kim JY, Yun TK, Morgan G, Vainio H. The cancerpreventive potential of Panax ginseng: a review of human and experimental evidence. Cancer Causes Control 2000; 11:565-576. https://doi.org/10.1023/A:1008980200583
  4. Yun TK, Lee YS, Lee YH, Kim SI, Yun HY. Anticarcinogenic effect of Panax ginseng C. A. Meyer and identifi - cation of active compounds. J Korean Med Sci 2001;16 Suppl:S6-S18. https://doi.org/10.3346/jkms.2001.16.S.S6
  5. Dey L, Xie JT, Wang A, Wu J, Maleckar SA, Yuan CS. Anti-hyperglycemic effects of ginseng: comparison between root and berry. Phytomedicine 2003;10:600-605. https://doi.org/10.1078/094471103322331908
  6. Kim SH, Park KS. Effects of Panax ginseng extract on lipid metabolism in humans. Pharmacol Res 2003;48:511-513. https://doi.org/10.1016/S1043-6618(03)00189-0
  7. Hu SY. The genus Panax (ginseng) in Chinese medicine. Econ Bot 1976;30:11-28. https://doi.org/10.1007/BF02866780
  8. Park JD, Rhee DK, Lee YH. Biological activities and chemistry of saponins from Panax ginseng C. A. Meyer. Phytochem Rev 2005;4:159-175. https://doi.org/10.1007/s11101-005-2835-8
  9. Lee BY. Status of Korean ginseng industry and development of new ginseng products. Food Ind Nutr 2003;8:1-9.
  10. Kwon WS, Chung CM, Kim YT, Lee MG, Choi KT. Breeding process and characteristics of KG101, a superior line of Panax ginseng C. A. Meyer. Korean J Ginseng Sci 1998;22:11-17.
  11. Kwon WS, Lee MG, Choi KT. Breeding process and characteristics of Yunpoong, a new variety of Panax ginseng C. A. Meyer. J Ginseng Res 2000;24:1-7.
  12. Kwon WS, Lee JH, Park CS, Yang DC. Breeding process and characteristics of Gopoong, a new variety of Panax ginseng C. A. Meyer. J Ginseng Res 2003;27:86-91. https://doi.org/10.5142/JGR.2003.27.2.086
  13. Joshi SP, Gupta VS, Aggarwal RK, Ranjekar PK, Brar DS. Genetic diversity and phylogenetic relationship as revealed by inter simple sequence repeat (ISSR) polymorphism in the genus Oryza. Theor Appl Genet 2000;100:1311-1320. https://doi.org/10.1007/s001220051440
  14. Chowdhury MA, Vandenberg B, Warkentin T. Cultivar identifi cation and genetic relationship among selected breeding lines and chickpea (Cicer arietinum L.). Euphytica 2002; 127:317-325. https://doi.org/10.1023/A:1020366819075
  15. Reddy MP, Sarla N, Siddiq EA. Inter simple sequence repeat (ISSR) polymorphism and its application in plant breeding. Euphytica 2002;128:9-17. https://doi.org/10.1023/A:1020691618797
  16. Pharmawati M, Yan G, Finnegan PM. Molecular variation and fi ngerprinting of Leucadendron cultivars (Proteaceae) by ISSR markers. Ann Bot 2005;95:1163-1170. https://doi.org/10.1093/aob/mci127
  17. Gostimskiĭ SA, Kokaeva ZG, Konovalov FA. Studying plant genome variation using molecular markers. Genetika 2005;41:480-492.
  18. Williams JG, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV. DNA polymorphisms amplifi ed by arbitrary primers are useful as genetic markers. Nucleic Acids Res 1990;18:6531-6535. https://doi.org/10.1093/nar/18.22.6531
  19. Deng Z, Xiao S, Huang S, Gmitter FG Jr. Development and characterization of SCAR markers linked to the Citrus tristeza virus resistance gene from Poncirus trifoliata. Genome 1997;40:697-704. https://doi.org/10.1139/g97-792
  20. Ardiel GS, Grewal TS, Deberdt P, Rossnagel BG, Scoles GJ. Inheritance of resistance to covered smut in barley and development of a tightly linked SCAR marker. Theor Appl Genet 2002;104:457-464. https://doi.org/10.1007/s001220100696
  21. In DS, Kim YC, Bang KH, Chung JW, Kim OT, Hyun DY, Cha SW, Kim TS, Seong NS. Genetic relationships of Panax species by RAPD and ISSR analyses. Korean J Med Crop Sci 2005;13:249-253.
  22. Kim OT, Bang KH, In DS, Lee JW, Kim YC, Shin YS, Hyun DY, Lee SS, Cha SW, Seong NS. Molecular authentication of ginseng cultivars by comparison of internal transcribed spacer and 5.8S rDNA sequences. Plant Biotechnol Rep 2007;1:163-167. https://doi.org/10.1007/s11816-007-0019-2

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