Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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Ginsenoside F1 Modulates Cellular Responses of Skin Melanoma Cells

  • Yoo, Dae-Sung (College of Biomedical Science and Institute of Bioscience and Biotechnology, Kangwon National University) ;
  • Rho, Ho-Sik (R&D Center, Amore-Pacific Co.) ;
  • Lee, Yong-Gyu (College of Biomedical Science and Institute of Bioscience and Biotechnology, Kangwon National University) ;
  • Yeom, Myung-Hun (R&D Center, Amore-Pacific Co.) ;
  • Kim, Duck-Hee (R&D Center, Amore-Pacific Co.) ;
  • Lee, Sang-Jin (Department of Genetic Engineering, Sungkyunkwan University) ;
  • Hong, Sung-Youl (Department of Genetic Engineering, Sungkyunkwan University) ;
  • Lee, Jae-Hwi (College of Pharmacy, Chung-Ang University) ;
  • Cho, Jae-Youl (Department of Genetic Engineering, Sungkyunkwan University)
  • Received : 2010.11.01
  • Accepted : 2010.11.10
  • Published : 2011.03.29
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Abstract

Ginsenoside (G)-F1 is an enzymatic metabolite generated from G-Rg1. Although this metabolite has been reported to suppress platelet aggregation and to reduce gap junction-mediated intercellular communication, the modulatory activity of G-F1 on the functional role of skin-derived cells has not yet been elucidated. In this study, we evaluated the regulatory role of G-F1 on the cellular responses of B16 melanoma cells. G-F1 strongly suppressed the proliferation of B16 cells up to 60% at 200 ${\mu}g/mL$, while only diminishing the viability of HEK293 cells up to 30%. Furthermore, G-F1 remarkably induced morphological change and clustering of B16 melanoma cells. The melanin production of B16 cells was also significantly blocked by G-F1 up to 70%. Interestingly, intracellular signaling events involved in cell proliferation, migration, and morphological change were up-regulated at 1 h incubation but down-regulated at 12 h. Therefore, our results suggest that G-F1 can be applied as a novel anti-skin cancer drug with anti-proliferative and anti-migration features.

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References

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