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Physiological Properties of Oak Mistletoe (Loranthus yadoriki) Extracts by Microwave Extraction Condition

마이크로웨이브 추출조건에 따른 참나무 겨우살이 추출물의 생리활성

  • Received : 2010.08.30
  • Accepted : 2011.01.07
  • Published : 2011.02.28

Abstract

The physiological properties of oak mistletoe slice extracts were investigated. The total polyphenol content of mistletoe extracts was 50.28-109-69 mg%, depending on extraction conditions. The polyphenol content increased as extract concentration rose (25 mL/g>50 mL/g>100 mL/g). The electron-donating ability (EDA) of the 60% (v/v) ethanolic extract was 68.88% at 25 mL/g; this was the highest value of all extracts tested (p<0.05). The SOD-like activities of water, 30%, 60%, and 90% (all v/v) ethanolic extracts were 15.24-30.84% lower than those of 1.0% and 0.1% (both w/v) L-ascorbate solutions. Tyrosinase inhibitory activities of all samples were 3.40-30.92% lower than those of 1.0% and 0.1% (both w/v) L-ascorbate solutions. Nitrite-scavenging abilities measured at pH 1.2 and pH 3.0 were highest when the water, and the 30%, 60% and 90% (all v/v) ethanolic extracts, were tested. The results expand our understanding of the physiological properties of oak mistletoe slice extracts.

참나무 겨우살이의 생리활성을 알아보기 위해 전자공여 작용, SOD 유사활성, 총 폴리페놀 함량, Tyrosinase 저해효과 및 아질산염 소거작용을 측정하였다. 전자공여능은 추출용매의 비율에 따라 농도가 높아짐에 따라 전자공여능도 증가하였고, 특히 60% EtOH을 추출용매로 하여 25 mL/g의 비율로 추출한 실험구가 68.88%로 가장 높은 항산화력을 나타냈다(p<0.05). SOD 유사활성 측정에서는 모든 추출물이 15.24-30.84%로 비교물질인 L-ascorbic acid 보다 낮은 활성을 나타냈다(p<0.05). 총 폴리페놀 함량 측정 결과 참나무 겨우살이의 페놀 함량이 50.28-109.69 mg%로 나타났다. 추출조건에 따라 25 mL/g>50 mL/g>100 mL/g으로 농도가 높을수록 페놀함량도 유의적으로 높아졌고, 추출용매에 따라 물 추출물에 비해 에탄올 추출물에서 다소 높은 경향을 보였다. 아질산염소거능을 측정한 결과 pH 1.2와 pH 3.0과 같은 산성조건에서 소거능이 모두 높았고, 추출용매와 상관없이 농도가 높은 추출구에서 아질산염 소거능이 높게 나타났다.

Keywords

References

  1. Kim OK (2005) Antidiabetic and antioxidative effects of Corni fructus in streptozotocin-induced diabetic rats. J Korean Oil Chemists Soc, 22, 157-167
  2. Lee JS, Park YC, Paik SW, Lee SS, Ahn YK, Lee JS (2008) Physiological functionality of Gugija products and an In vivo examination on anti-hpertension effects. Korean J Food Nutr, 21, 115-120
  3. Jeong MH, Choi WY, Seo YC, Kang HY, Choi G.P Lee HY (2010) Anticancer activity of Acer mono wood extracted by ultra high pressure extraction process. Korean J Medicinal Crop Sci, 18, 157-167
  4. Park YH, Chang SK (1997) Screening of inhibitory effect of edible mushrooms on tyrosinase and isolation of active component. J Food Hyg Safety, 12, 195-199
  5. Lee SO, Lee J, Yu MH, Im HG, Lee IS (2005) Total polyphenol contents and antioxidant activities of methanol extracts from vegetables produced in Ullung island. Korean J Food Sci Technol, 37, 233-240
  6. Park YS, Jang HG (2003) Lactic acid fermentation and biological activities of rubus coreanus. J Korean Soc Agric Chem Biotechnol, 46, 367-375
  7. Gayon GR, Jung ML, Scala DD, Beck JP (1986) Comparison of the effects of fermented and unfermented mistletoe preparations on cultured tumor cells. Oncology, 43, 35-46 https://doi.org/10.1159/000226418
  8. Holtskog R, Sandvig K, Olsnes S (1988) Characterization of a toxic lectin in Isocador, a misltetoe preparation with alleged cancerostatic properties. Oncology, 45, 172-179 https://doi.org/10.1159/000226557
  9. Pare JRJ, Sigouin M, Lapointe J (1991) Microwave-assisted natural products extraction. US Patent 5002, Various international counterparts, 26, 784
  10. Jocelyn Pare JR, Belanger MR, Stafford SS (1994) Microwave-assosted process(MAPTM) : a new tool for the analytical laboratory. Trend in analytical Chemisty, 13, 176-184 https://doi.org/10.1016/0165-9936(94)87033-0
  11. Giese J (1992) Advances in microwave food processing. Food Technol, 46, 118-123
  12. Folin O, Denis W (1912) On Phosphotungasticphosphomo- lybdic compounds as color reagents. J Biol Chem, 12, 239-249
  13. Blois MS (1958) Antioxidant determination by the use of a stable free radical. Nature, 26, 1198-1202
  14. Marklund S, Marklund G (1975) Involvement of superoxide amino radical in the oxidation of pyrogallol and a convenient assay for superoxide dismutase. European J Biochem, 47, 468-474
  15. Gray JI, Dugan Jr LR (1975) Inhibition of N-nitosamine formation in model food system. J Food Sci, 40, 981-984 https://doi.org/10.1111/j.1365-2621.1975.tb02248.x
  16. Wong TC, Luh BS, Whitaker JR (1971) Isolation and charaterization of polyphenol oxidase of clingstone peach. Plant Physiol, 48, 19-23 https://doi.org/10.1104/pp.48.1.19
  17. Joo EY, Kim NW (2008) Polyphenol contents and antioxidant activity of extracts from Angelica dahurica root after different condition of microwave-assisted extraction. Korean J Food Preserv, 15, 133-138
  18. Kim TH (2008) Antioxidative and biological activities of Santalum album extracts by extracting methods. Korean J Food Preserv, 15, 456-460
  19. Seo SJ, Kim NW (2010) Physiological activities of leaf and root extracts from Liriope platyphylla. Korean J Food Preserv, 17, 123-130
  20. Lee YS (2007) Antioxidative and physiological activity of extracts of Angelica dahurica leaves. Korean J Food Preserv, 14, 78-86
  21. Na GM, Han HS, Ye SH, Kim HG (2004) Physiological activity of medicinal plant extracts. Korean J Food Preserv, 11, 388-393
  22. Torel J, Gillard J, Gillard P (1986) Antioxidant activity of flavonoids and reactivity with peroxy radical. Phytochemistry, 25, 383-385 https://doi.org/10.1016/S0031-9422(00)85485-0
  23. Lee HJ, Do JR, Kown JH, Kim HG (2010) Antioxidant effect of Viscum album L. extracts by extraction conditions. J Korean Soc Food Sci Nutr, 39, 14-19 https://doi.org/10.3746/jkfn.2010.39.1.014
  24. Ye EJ, Kim SJ, Nam HS, Park EM, Bae MJ (2010) Physiological ecalution of korean mountain ginseng and korean mountain ginseng leaf tea. Korean J Food Culture, 25, 350-356
  25. Lee SE, Kim YS, Kim JE, Bang JK, Seong, N.S. (2004) Antioxidant activity of Ulmus davidiana var. jiponica N. and Hemipteleae davidii P. Korean J Medicinal Crop Sci, 12, 321-327
  26. Davies KJ, Goldberg AL (1987) Proteins damaged by oxygen radicals are rapidly degraded in extracts of red blood cells. J Biol Chem, 262, 8227-8234
  27. Aruoma OI (1994) Nutrition and health aspects of free radicals and antioxidants. Food Chem Toxicol, 32, 671-683 https://doi.org/10.1016/0278-6915(94)90011-6
  28. Park CS, Kim DH, Kim ML (2008) Biological activities of extracts from Corni fructus, Astragalus membranaceus and Glycyrrhiza uralensis. Korean J Herbology, 23, 93-101
  29. Jeong SJ, Lee JH, Song HN, Seong NS, Lee SE, Baeg NI (2004) Screening for antioxidant activity of plant medicinal extracts. J Korean Soc Appl Biol Chem, 47, 135-140
  30. Lim JD, Yu CY, Kim MJ, Yun SJ, Lee SJ, Kim NY, Chung IM (2004) Comparison of SOD activity and phenolic compound contents in various Korean medicinal plants. Korean J Medicinal Crop Sci, 12, 191-202
  31. Kim SS, Ha JH, Jeong MM, Ahn JH, Yoon WB, Park SJ, Seong DH, Lee HY (2009) Comparison of biological acitivities of fermented Codonopsis lanceolata and fresh Codonopsis lanceolata. Korean J Medicinal Crop Sci, 17, 280-285
  32. Jung SW, Lee NK, Kim SJ, Han DS (1995) Screening of tyrosinase inhibitor from plants. Korean J Food Sci Technol, 27, 891-896
  33. Kim JH, Jeong CH, Shim KH (2010) Antioxidative and anticancer activities of various solvent fractions from the leaf of Camellia japonica L. Korean J Food Preserv, 17, 267-274
  34. Song HS, Kim DP, Jung YH, Lee MK (2007) Antioxidant activities of red hamcho(Salicornia herbacea L.) against lipid peroxidation and the formation of radicals. Korean J Food Nutr, 20, 150-157
  35. Park YB, Lee TG, Kim WK, Do JR, Yeo SG, Park YH, Kim SB (1995) Characteristics of nitrite scavenger derived from seeds of Cassia tara L. Korean J Food Sci Technol, 27, 124-128

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