DOI QR코드

DOI QR Code

Microbial Inactivation of Chicken Cage Litter by Aqueous Chloride Dioxide

이산화염소수를 이용한 계사 내 깔짚의 미생물 수 저감화

  • Yu, Dong-Jin (Department of Food Science and Technology, Chungnam National University) ;
  • Kim, Hyun-Jin (Department of Food Science and Technology, Chungnam National University) ;
  • Song, Hyeon-Jeong (Department of Food Science and Technology, Chungnam National University) ;
  • Shin, Yoon-Ji (Department of Food Science and Technology, Chungnam National University) ;
  • Chae, Hyun-Seok (Poultry Science Division, National Institute of Animal Science) ;
  • Song, Kyung-Bin (Department of Food Science and Technology, Chungnam National University)
  • 유동진 (충남대학교 농업생명과학대학 식품공학과) ;
  • 김현진 (충남대학교 농업생명과학대학 식품공학과) ;
  • 송현정 (충남대학교 농업생명과학대학 식품공학과) ;
  • 신윤지 (충남대학교 농업생명과학대학 식품공학과) ;
  • 채현석 (국립축산과학원) ;
  • 송경빈 (충남대학교 농업생명과학대학 식품공학과)
  • Received : 2010.07.30
  • Accepted : 2010.12.31
  • Published : 2011.02.28

Abstract

We evaluated microbial inactivation in chicken cage litter, to ensure microbial safety, using aqueous chloride dioxide. Contamination by coliforms, Escherichia coli, Listeria spp., yeasts and molds, total aerobic bacteria, and Salmonella spp. was detected in fresh cage litter, and microbial populations increased if litters were repeatedly used. Aqueous $ClO_2$ treatment (500 ppm) significantly decreased the populations of coliforms, E. coli, Listeria spp., yeasts and molds, total aerobic bacteria, and Salmonella spp. in all litter samples tested. In particular, aqueous $ClO_2$ treatment on fresh litter reduced the initial populations of coliform, E. coli, Listeria spp., yeasts and molds, and total aerobic bacteria by 4.47, 1.29, 1.23, 3.24, and 5.2 log CFU/g, respectively. In addition, when litters used for 1 and 5 weeks were tested, treatment significantly reduced microbial populations. The results suggest that aqueous $ClO_2$ treatment is useful to reduce microbial hazards in chicken cage litter and to improve the microbial safety of slaughtered chickens.

본 연구에서는 이산화염소수를 이용한 계사 내 깔짚의 미생물 수 저감화를 통한 깔짚의 미생물학적 안전성 확보를 위하여 연구를 수행하였다. 사용하기 전의 깔짚에는 coliform, E. coli, Listeria spp., yeasts and molds 및 total aerobic bacteria가 높은 수준으로 검출되었으며 사용기간이 늘어남에 따라 미생물 수가 증가하였다. 500 ppm 이산화염소수를 이용한 살균처리에서는 coliform, E. coli, Listeria spp., yeasts and molds, total aerobic bacteria 및 Salmonella spp.의 균수가 모든 깔짚 시료에서 처리횟수에 따라 유의적으로 감소하였다. 사용하지 않은 깔짚에서는 coliform, E. coli, Listeria spp., yeasts and molds 및 total aerobic bacteria 수가 각각 4.47, 1.29, 1.23, 3.24 및 5.2 log CFU/g로 감소하였고, 1주 및 5주 사용한 깔짚의 경우도 모든 미생물 수가 현저하게 감소하였다. 따라서 본 연구 결과, 이산화염소수를 이용한 계사 내 깔짚의 미생물 수 저감화 방법이 도계된 계육의 미생물학적 안전성을 향상시키기 위한 방법으로 적합하다고 판단된다.

Keywords

References

  1. Patsias A, Chouliara I, Badeka A, Savvaidis IN, Kontominas MG (2006) Shelf-life of a chilled precooked chicken product stored in air and under modified atmospheres: Microbiological, chemical, sensory attributes. Food Microbiol, 23, 423-429 https://doi.org/10.1016/j.fm.2005.08.004
  2. Chouliara E, Karatapanis A, Savvaidis IN, Kontominas MG (2007) Combined effect of oregano essential oil and modified atmosphere packaging on shelf-life extension of fresh chicken breast meat, stored at $4^{\circ}C$. Food Microbiol, 24, 607-617 https://doi.org/10.1016/j.fm.2006.12.005
  3. Hong YH, Ku KJ, Kim MK, Won MS, Chung KS, Song KB (2008) Survival of Escherichia coli O157: H7 and Salmonella typhimurium inoculated on chicken by aqueous chlorine dioxide treatment. J Microbiol Biotechnol, 18, 742-745
  4. Kim HJ, Chun HH, Kim JY, Jang SA, Lee BD, Chae HS, Song KB (2010) Identification of microorganisms isolated from wound regions of chickens. Korean J Food Preserv, 17, 301-306
  5. Davies RH, Wray C (1996) Determination of an effective sampling regime to detect Salmonella enteritidis in the environment of poultry units. Vet Microbiol, 50, 117-127 https://doi.org/10.1016/0378-1135(96)00031-4
  6. Rose N, Beaudeau F, Drouin P, Toux JY, Rose V, Colin P (1999) Risk factors for Salmonella enterica subsp. Enterica contamination in french broiler-chicken flocks at the end of the rearing period. Prev Vet Med, 39, 265-277 https://doi.org/10.1016/S0167-5877(99)00002-1
  7. Park KJ, Park EJ, Kim JO, Kim YH (1995) Changes in the microflora on the surface of chicken meat during chilled and frozen storage. Korean J Anim Sci, 37, 279-286
  8. Owusu-Yaw J, Toth J, Wheeler W, Wei C (1990) Mutagenicity and identification of the reaction products of aqueous chlorine or chlorine dioxide with l-tryptophan. J Food Sci, 55, 1714-1719 https://doi.org/10.1111/j.1365-2621.1990.tb03607.x
  9. Kim JM (2001) Use of chlorine dioxide as a biocide in the food industry. Food Ind Nutr, 6, 33-39
  10. Rand JL, Hofmann R, Alam MZB, Chauret C, Cantwell R, Andrews RC, Gagnon GA (2007) A field study evaluation for mitigating biofouling with chlorine dioxide or chlorine integrated with UV disinfection. Water Res, 41, 1939-1948 https://doi.org/10.1016/j.watres.2007.02.004
  11. American Public Health Association (1995) Standard methods for the examination of water and wastewater. 19th ed, American Public Health Association, Washington DC, p 4-54
  12. SAS (2001) The SAS system for windows. Version 8.2. Statistical Analysis System Institute, Cary, NC, USA
  13. Kelley T, Pancorbo O, Merka W, Thompson S, Cabrera M, Barnhart H (1995) Bacterial pathogens and indicators in poultry litter during reutilization. J Appl Poult Res, 4, 366-373 https://doi.org/10.1093/japr/4.4.366
  14. Tamblyn K, Conner D, Bilgili S (1997) Utilization of the skin attachment model to determine the antibacterial efficacy of potential carcass treatments. Poult Sci, 76, 1318-1323 https://doi.org/10.1093/ps/76.9.1318
  15. Arshad N, Neubauer C, Hasnain S, Hess M (2008) Peganum harmala can minimize Escherichia coli infection in poultry, but long-term feeding may induce side effects. Poult Sci, 87, 240-249 https://doi.org/10.3382/ps.2007-00341
  16. Hong YH, Ku GJ, Kim MK, Song KB (2008) Effect of aqueous chlorine dioxide treatment on the microbial growth and quality of chicken legs during storage. J Food Sci Nutr, 13, 45-50 https://doi.org/10.3746/jfn.2008.13.1.045
  17. Hong YH, Ku GJ, Kim MK, Song KB (2007) Inactivation of Listeria monocytogenes and Campylobacter jejuni in chicken by aqueous chlorine dioxide treatment. J Food Sci Nutr, 12, 279-283 https://doi.org/10.3746/JFN.2007.12.4.279
  18. Hong YH, Kim MK, Song KB (2008) Effects of aqueous chlorine dioxide treatment on microbial safety and quality of Samgae chicken. Korean J Food Preserv, 15, 769-773

Cited by

  1. Enhanced antimicrobial effectiveness of Omija (Schizamdra chinesis Baillon) by ClO2(chlorine dioxide) treatment vol.20, pp.6, 2013, https://doi.org/10.11002/kjfp.2013.20.6.871