Clinical Application of Mesenchymal Stem Cells in a Dog with Intervertebral Disc Disease

추간판 탈출증이 발생한 개에서 동종의 지방유래 줄기세포의 임상적 적용

  • Kim, Young-Ki (Lab. of Veterinary Surgery and Behavior, College of Veterinary Medicine, Gyeongsang National University) ;
  • Lee, Seung-Yong (Lab. of Veterinary Surgery and Behavior, College of Veterinary Medicine, Gyeongsang National University) ;
  • Park, Se-Jin (Lab. of Veterinary Surgery and Behavior, College of Veterinary Medicine, Gyeongsang National University) ;
  • Lee, Scott-S. (Lab. of Veterinary Surgery and Behavior, College of Veterinary Medicine, Gyeongsang National University) ;
  • Kim, Jin-Hyun (Clinical Research Institute, Gyeongsang National University Hospital) ;
  • Lee, Hee-Chun (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University) ;
  • Chang, Hong-Hee (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University) ;
  • Lee, Hyo-Jong (Lab. of Veterinary Surgery and Behavior, College of Veterinary Medicine, Gyeongsang National University) ;
  • Yeon, Seong-Chan (Lab. of Veterinary Surgery and Behavior, College of Veterinary Medicine, Gyeongsang National University)
  • 김영기 (경상대학교 수의과대학 수의외과, 행동학 연구실) ;
  • 이승용 (경상대학교 수의과대학 수의외과, 행동학 연구실) ;
  • 박세진 (경상대학교 수의과대학 수의외과, 행동학 연구실) ;
  • 이스캇 (경상대학교 수의과대학 수의외과, 행동학 연구실) ;
  • 김진현 (경상대학교 병원 임상의학연구소) ;
  • 이희천 (경상대학교 수의과대학 동물의학연구소) ;
  • 장홍희 (경상대학교 수의과대학 동물의학연구소) ;
  • 이효종 (경상대학교 수의과대학 수의외과, 행동학 연구실) ;
  • 연성찬 (경상대학교 수의과대학 수의외과, 행동학 연구실)
  • Accepted : 2011.01.17
  • Published : 2011.02.28

Abstract

A 5-year old, intact male, Cocker spaniel dog was referred with paraplegia and loss of deep pain perception. Physical, neurological examinations, radiography, and computed tomography were evaluated. Based on the clinical examinations, the dog was diagnosed with severe disc herniation ($L_2$ to $L_3$ intervertebral disc space). On the next day of presentation (6 days after loss of deep pain perception), hemilaminectomy was performed. After decompression of spinal cord and removal of herniated disc materials, $1{\times}10^6$ canine allogenic adipose tissue-derived mesenchymal stem cells (MSCs) diluted by $50{\mu}l$ saline were directly applied to the injured site of the spinal cord. Ten weeks of follow-up after surgery, full recovery of deep pain perception and motor function were evaluated in both hind limbs. Based on the result, we suggest that the transplantation of allogenic adipose tissue-derived MSCs to dogs with spinal cord injuries could be a considerable method to expect better clinical outcomes in veterinary practice.

후지마비와 심부통각의 소실을 보이는 5 년령의 수컷 Cocker Spaniel이 내원하였다. 신체검사, 신경검사, 방사선검사와 컴퓨터 단층촬영결과 심부통각의 소실을 동반한 요추 2번과 3번 사이의 디스크 탈출증으로 진단하였다. 편측 추궁 절제술을 시행하여 감압술과 탈출된 디스크 물질을 제거하였으며 $1{\times}10^6$ 개의 동종 지방유래 줄기세포를 $50{\mu}l$ 생리식염수에 희석하여 손상된 척수에 직접 주입하였다. 수술 10주 후, 양쪽 후지 모두에서 심부통각과 운동기능이 완전히 회복되었으며 세포이식과 관련된 부작용은 현재까지 발견되지 않았다. 본 증례를 통해 심각한 정도의 디스크질환을 가진 환축에서 감압술과 병행된 줄기세포의 이식방법은 좀더 나은 예후를 기대하기 위한 치료방법들 중 하나로 고려해볼 수 있을 것으로 생각된다.

Acknowledgement

Supported by : National Research Foundation of Korea (NRF)

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