Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Evaluation of Biological Activity in Pepper (Capsicum annuum L.) Breeding Lines

육성계통에 따른 고추의 생리활성 평가

  • Jung, Mi-Ri (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Hwang, Young (Korean Food & Culture Division, RDA) ;
  • Kim, Hae-Young (Dept. of Food Science and Nutrition, Yong In University) ;
  • Cho, Myeong-Cheoul (National Institute of Horticultural and Herbal Science, RDA) ;
  • Hwang, In-Guk (Korean Food & Culture Division, RDA) ;
  • Yoo, Seon-Mi (Korean Food & Culture Division, RDA) ;
  • Jeong, Heon-Sang (Dept. of Food Science and Technology, Chungbuk National University) ;
  • Lee, Jun-Soo (Dept. of Food Science and Technology, Chungbuk National University)
  • 정미리 (충북대학교 식품공학과) ;
  • 황영 (농촌진흥청 농식품자원부) ;
  • 김혜영 (용인대학교 식품영양학과) ;
  • 조명철 (농촌진흥청 원예작물부) ;
  • 황인국 (농촌진흥청 농식품자원부) ;
  • 유선미 (농촌진흥청 농식품자원부) ;
  • 정헌상 (충북대학교 식품공학과) ;
  • 이준수 (충북대학교 식품공학과)
  • Received : 2011.03.04
  • Accepted : 2011.04.16
  • Published : 2011.05.31
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Abstract

The purpose of this study was to determine the capsaicinoid and antioxidant compounds and to evaluate the biological activity of 40 different pepper (Capsicum annuum L.) breeding lines. Capsaicinoid and ascorbic acid content were measured with HPLC. Total polyphenolic and flavonoid contents and antioxidant activity were measured with spectrophotometric methods. The antiproliferative qualities of the samples against human breast tumor cells (MCF7) were assessed with a MTT assay. The nitric oxide content in the culture media was measured to evaluate the anti-inflammatory qualities of the samples using Raw264.7 macrophages. The capsaicinoid, ascorbic acid, polyphenolic and flavonoid content of the 40 pepper breeding lines were 0.1~204.2 mg/100 g (dry weight basis, DWB), 279.1~1695.5 mg/g (DWB), 2.6~10.2 mg/g (DWB), and 1.4~5.7 mg/g (DWB), respectively. The highest antioxidant, antiproliferative, and anti-inflammatory qualities were found in breeding line numbers 2500, 3201, and 3232, respectively. This study provides basic information useful to plant breeders and biotechnologists who are planning to breed pepper genotypes with high phytochemical content.

본 연구에서는 40여종의 육성계통 고추의 기능성 성분의 함량을 분석 및 활성 평가를 함으로써 우수 고추 품종 개발을 위한 database를 제공하고자 하였다. 연구 결과 capsaicinoid의 함량은 0.1에서 204.2 mg/100 g으로 나타났으며 ascorbic acid 함량은 279.1에서 1695.5 mg/100 g으로 넓은 분포 범위를 보였다. Polyphenol 함량은 2.6에서 10.2 mg/g 수준으로, flavonoid 함량은 1.4에서 5.7 mg/g 수준으로 나타났다. 항산화 활성 측정 결과는 육성계통 고추 breeding line No. 2500은 다른 계통에 비해 가장 높은 DPPH와 ABTS 라디칼 제거능을 나타내었으며 polyphenol과 flavonoid 함량 및 환원력이 다른 육성계통에 비해 높은 값을 나타내었다. 항암 효과는 breeding line No. 3201이 암세포 증식 저해율 84.5%로 가장 높은 효과를 나타내었으며 항염증 효과는 breeding line No. 3232가 75.2%로 가장 높은 nitric oxide 생성 억제율을 확인하였다. 본 연구에서는 육성계통에 따른 고추의 기능성 성분을 분석하고 다양한 생리활성을 평가하여 우수 품종 개량을 위한 성분 및 활성 database로 활용될 수 있을 것으로 생각된다.

Keywords

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