Journal of the East Asian Society of Dietary Life (동아시아식생활학회지)

The East Asian Society of Dietary Life (동아시아식생활학회)
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The Effect of Angelica keiskei Ethnol Extract on Proliferation, Apotosis and ROS Accumulation in Human Breast Cancer MDA-MB-231 Cells

신선초 에탄올 추출물이 인체 유방암 MDA-MB-231 세포에서 세포증식, 세포사멸과 ROS 축적에 미치는 영향

  • Jeong, Yu-Jin (Dept. of Food & Nutrition, College of Natural Science, DukSung Women's University) ;
  • Nam, Mi-Kyung (Dept. of Food & Nutrition, College of Natural Science, DukSung Women's University) ;
  • Kang, Keum-Jee (Dept. of Food & Nutrition, College of Natural Science, DukSung Women's University)
  • 정유진 (덕성여자대학교 자연과학대학 식품영양학과) ;
  • 남미경 (덕성여자대학교 자연과학대학 식품영양학과) ;
  • 강금지 (덕성여자대학교 자연과학대학 식품영양학과)
  • Received : 2010.11.15
  • Accepted : 2011.02.14
  • Published : 2011.02.28
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Abstract

The anti-cancer effects of Angelica keiskei ethanol extract were evaluated in human breast cancer MDA-MB-231 cells. The concentrations of extract were 1, 2, 3, 4 and 5 mg/mL. Dose-dependent reductions in the number of cells with altered cell shape and pyknotic nuclei were observed at 48 h after treatments. MTT assay also exhibited a similar dose-dependent reduction in mitochondrial reductase activity (p<0.05), in particular, with a rapid reduction in the activity of the 5 mg/mL group. Analysis of cell death with propidium iodide (PI) staining revealed only a slight increase in cell death in the 5 mg/mL group. Analysis of bromodeoxyuridine (BrdU) incorporations also showed a dose-dependent reduction in cell proliferation (p<0.05). Finally, increases in total radical oxygen species (ROS) accumulation in cells, as revealed by DCF-DA staining, were observed in the treated groups in a similar dose-dependent fashion (p<0.05). These results indicate that Angelica keiskei ethanol extract exhibiting anti-cancer effects in MDA-MB-231 cells causes multiple changes in cell shape, enzyme activity, and ROS accumulation, thereby inducing cell death.

Keywords

References

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