한국작물학회지 (KOREAN JOURNAL OF CROP SCIENCE)

  • 제56권1호
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  • Pages.23-28
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  • 2011
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  • 0252-9777(pISSN)
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  • 2287-8432(eISSN)
한국작물학회 (The Korean Society of Crop Science)
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뽕잎의 품종별.시기별 resveratrol 함량 변이

Quantitative Analysis of Resveratrol in Mulberry Leaves

  • 투고 : 2010.09.06
  • 발행 : 2011.03.30
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초록

뽕 품종의 육종 효율을 높이는 동시에 뽕잎의 기능성 및 이용성을 증대시키기 위해 품종별 채취시기별로 뽕잎을 채취하여 기능성물질인 레스베라트롤 함량을 분석하였다. 그 결과, 오디에 비해 상당히 낮은 함량을 나타냈으나 레스베라트롤의 주요 공급원으로 알려진 포도 품종과 비교했을 때에는 추기의 뽕잎은 오히려 평균 함량이 4배 높았다. 채취시기별 뽕잎의 평균 레스베라트롤 함량에 있어서는 추기의 뽕잎이 춘기보다 함량이 높았으며, 춘기의 뽕잎은 어린잎보다 성숙잎의 레스베라트롤 함량이 높았다. 즉 춘기 5개엽기 어린잎의 평균 레스베라트롤 함량은 $102{\mu}g/100g$이었으며 뽕잎의 성숙시기가 경과함에 따라 165, 209, $359{\mu}g/100g$으로 증가하였다. 하벌 후 새로 자란 뽕잎을 8윌 중순에 채취한 것은 $466{\mu}g/100g$이었으며, 10월 중순의 뽕잎은 $483{\mu}g/100g$ 이었다. 특히 10월 중순에 채취한 뽕잎은 엽질이 매우 거칠기 때문에 직접 식용으로의 이용 가능성은 매우 적으나 '개량뽕', '수계뽕' 및 '청일뽕' 품종의 경우 뽕잎의 레스베라트롤 함량이 각각 $838{\mu}g/100g$, $803{\mu}g/100g$, $800{\mu}g/100g$으로 매우 높으므로 기능성 화장품 등에 첨가하여 사용하기 위한 레스베라트롤 추출물 등을 위한 시료로서 이용한다면 그 활용 가능성은 매우 클 것으로 판단하였다. 또한 '신일뽕', '수성뽕' 및 '상일뽕'의 레스베라트롤 함량도 각각 $794{\mu}g/100g$, $606{\mu}g/100g$, $583{\mu}g/100g$으로 높았다. '청일뽕' 품종의 뽕잎에 대한 수확 후 관리방법에 따른 레스베라트롤의 함량을 분석한 결과 뽕잎 채취 후 $-70^{\circ}C$에서 냉동, 동결건조하는 방법보다는 수세 후 바로 실온에서 음건하는 경우 뽕잎의 레스베라트롤 함량이 높았다.

Resveratrol has been associated with reduced cardiovascular disease and reduced cancer risk. This phytochemical has been reported in a number of plant species including grapes, peanuts and pine trees in response to stress such as fungal infection, heavy metal ions or UV irradiation. The objective of this study was to determine the resveratrol contents in leaves of mulberry varieties at different collecting times. Quantitative analysis of 16 cultivars showed a range of $102{\sim}466{\mu}g/100g$ on dry weight basis (which is equivalent to $25{\sim}116.5{\mu}g/100g$ on fresh weight basis). Resveratrol contents in mulberry leaves was higher in autumn than spring, and higher in fully matured leaves than in juvenile leaves. Among the tested samples, 'Kaeryangppong', 'Sugeppong' and 'Cheongilppong' collected in the middle of October showed high resveratrol contents of $838{\mu}g/100g$, $803{\mu}g/100g$, $800{\mu}g/100g$ on dry weight basis, respectively. Especially mulberry leaves dried in the shade showed of $1,030{\mu}g/100g$ on dry weight basis in resveratrol content, this result may contribute to utilization of mulberry leaves.

키워드

참고문헌

  1. Arora, M. K. and R. N. Strange. 1991. Phytoalexin accumulation in groundnuts in response to wounding. Plant Sci. 78: 157-163. https://doi.org/10.1016/0168-9452(91)90194-D
  2. Creasy, L. L. and M. Coffee. 1988. Phytoalexin production potential of grape berries. J. Am, Soc. Hortic. Sci. 113: 230-234.
  3. Dixon, R. A. 2001. Natural products and plant disease resistance. Nature. 411: 843-847. https://doi.org/10.1038/35081178
  4. Fontecave, M., M. Lepoivre, E. Elleingand, C. Gerez, and O. Guittet. 1998. Resveratrol, a remarkable inhibitor of ribonucleotide reductase. FEBS Lett., Jan. 16; 421(3): 277-279. https://doi.org/10.1016/S0014-5793(97)01572-X
  5. Fremont, L. 2000. Biological effects of resveratrol. Life Sci. 66: 663-673. https://doi.org/10.1016/S0024-3205(99)00410-5
  6. Fremont, L., L. Belguendou, and S. Delpal. 1999. Antioxidant activity of resveratrol and alcohol-free wine polyphenols realted to LDL oxidation and polyunsaturated fatty acids. Life Sci. 64: 2511-2521. https://doi.org/10.1016/S0024-3205(99)00209-X
  7. Ingham, J. L. 1976. 3,5,4'-Trihydroxystilbene as a phytoalexin from groundnuts (Arachis hypogaea). Phytochemistry. 15: 1791-1793. https://doi.org/10.1016/S0031-9422(00)97494-6
  8. Jeandel, P., R. Bessis, and B. Gautheron. 1991. The production of resveratrol (3,5,4'-thrhydroxystillbene) by grape berries in different developmental stages. Am. J. Enol. Vitic. 41: 41-46.
  9. Keen, N. T. 1975. The isolation of phytoalexins from germinating seeds of Cicer arictinum, Vigna sinensis, Arachis hypogaea and other plants. Phytopathology. 65 ; 91-92. https://doi.org/10.1094/Phyto-65-91
  10. Kim, D. J., S. G. Kim, M. H. Kim, H. B. Lee, and J. S. Lee. 2003. Analysis od trans-Resveratrol Contents of Grape and Grape Products Consumed in Korea. KOREAN J. FOOD SCI. TECHNOL 35(5): 764-768.
  11. Kim, H. B. 2005. Anti-oxidative Capacity Analysis of Water- Soluble Substances According to Varieties and Maturity Stages in Mulberry Leaves and Fruits. Korean J. Seric. Sci. 47(2): 62-67.
  12. Kim, H. B., J. B. Kim, and S. L. Kim. 2005. Varietal Analysis and Quantification of Resveratrol in Mulberry Fruits. Korean J. Seric. Sci. 47(2): 51-55.
  13. Kim, H. B. and S. L. Kim. 2003. Identification of C3G (cyanidin-3-glucoside) from Mulberry Fruits and Quantification with Different Varieties. Korean J. Seric. Sci. 45(2): 90-95.
  14. Kim, H. B. and S. L. Kim. 2004. Quantification and Varietal Variation of Rutin in Mulberry Fruits. Korean J. Seric. Sci. 46(1): 1-5.
  15. Kim, H. B., S. L. Kim, G. B. Sung, H. W. Nam, S. J. Chang and J. Y. Moon. 2003. Quantification and Varietal Variation of Fatty Acids in Mulberry Fruits. Korean J. Seric. Sci. 45(2): 75-79.
  16. Kim H. B., S. L. Kim, and S. W. Kang. 2004. Varietal Analysis and Quantification of Amino Acid in Mulberry Fruits. Korean J. Seric. Sci. 46(2): 47-53.
  17. Lee, H. S., E. Y. Sur, and W. K. Kim. 2004. Resveratrol Induces Apoptosis in SW480 Human Colon Cancer Cell Lines. Food Sci. Biotechnol. 13(1): 80-84.
  18. MacCarrone, M., T. Lorenzon, P. Guerrieri, and A. F. Agro. 1999. Resveratrol prevents apoptosis in K562 cells by inhibiting lipoxygenase and cyclooxygenase activity. Eur. J. Biochem. 265: 37-34.
  19. Pace-Asciak, C. R., S. E. Hahn, E. P. Diamandis, G. Soleas, and D. M. Goldberg. 1995. The red wine phenolics transresveratrol and quercetin block human platelet aggregation and eicosanoid synthesis: implications for protection against coronary heart disease. Clin. Chim. Acta. 235: 207-219. https://doi.org/10.1016/0009-8981(95)06045-1
  20. Ragione, F. D., V. Cucciolla, A. Borriello, V. D. Pietra, L. Racioppi, G. Soldati, C. Manna, P. Galletti, and V. Zappia. 1998. Resveratrol arrests the cell division cycle at S/G2 phase transition. Biochem. Biophys. Res. Commun., Sep. 8; 250(1): 53-58. https://doi.org/10.1006/bbrc.1998.9263
  21. Sanders, T. H. and R. W. McMichael. 1997. Occurrence of resveratrol in edible peanuts. Book of Abstract, Las Vegas, NV; American Chemical Society: Washington, DC. Abstr. AGFD 214:33.
  22. Sanders, T. H., R. W. McMichael, and K. W. Hendrix. 2000. Occurrence of Resveratrol in Edible Peanuts. J. Agric. Food Chem. 48: 1243-1246. https://doi.org/10.1021/jf990737b
  23. Sobolev, V. S. and R. J. Cole. 1999. trans-Resveratrol content in commercial peanuts and peanut products. J. Agric. Food Chem. 47: 1435-1439. https://doi.org/10.1021/jf9809885
  24. Sobolev, V. S., R. J. Cole, J. W. Dorner, and B. Yagen. 1995. Isolation, purification, and liquid chromatographic determination of stilbene phytoalexins in peanuts. J. Assoc. Off. Anal. Chem. Int. 78: 1177-1182.
  25. Subbaramaiah, K., P. Michaluart, W. J. Chung, T. Tanabe, N. Telang, and A. J. Dannenberg. 1999. Resveratrol inhibits cyclooxygenase-2 transcription in human mammary epithelial cell. Ann. N Y Acad. Sci. 889: 214-223. https://doi.org/10.1111/j.1749-6632.1999.tb08737.x
  26. 농촌진흥청 국립농업과학원. 2008. 2008년도 국립농업과학원 시험연구사업보고서-농업생물연구 I- p.788.

피인용 문헌

  1. Seasonal Resveratrol Contents of Wild-type Mulberry Leaves Collected from Gangwon Province in Korea vol.50, pp.1, 2012, https://doi.org/10.7852/jses.2012.50.1.10
  2. Bioactivity analysis of Resveratrol from peanut sprouts using On-line screening HPLC-ABTS vol.14, pp.8, 2013, https://doi.org/10.5762/KAIS.2013.14.8.4100
  3. Comparison with various mulberry leaves' and fruit's extract in lipid accumulation inhibitory effect at adipocyte model vol.35, pp.1, 2011, https://doi.org/10.7852/ijie.2017.35.1.1