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대두 추출물 LES가 DNCB로 유도된 랫드의 알레르기성 접촉피부염에 미치는 영향

Effect of LES on Recovery Capability of DNCB-Induced Allergic Contact Dermatitis in Rat

  • 김정숙 (안동대학교 자연과학대학 생명과학과) ;
  • 박영미 (안동대학교 자연과학대학 생명과학과) ;
  • 정진부 (안동대학교 자연과학대학 생약자원학과) ;
  • 임재환 (안동대학교 자연과학대학 생명과학과) ;
  • 정형진 (안동대학교 자연과학대학 생약자원학과) ;
  • 서을원 (안동대학교 자연과학대학 생명과학과)
  • Kim, Jeong-Sook (Department of Biological Science, Andong National University) ;
  • Park, Young-Mi (Department of Biological Science, Andong National University) ;
  • Jeong, Jin-Boo (Department of Medicinal Plant Resource, Andong National University) ;
  • Lim, Jae-Hwan (Department of Biological Science, Andong National University) ;
  • Jeong, Hyung-Jin (Department of Medicinal Plant Resource, Andong National University) ;
  • Seo, Eul-Won (Department of Biological Science, Andong National University)
  • 투고 : 2011.02.07
  • 심사 : 2011.04.08
  • 발행 : 2011.05.30

초록

본 연구에서는 대두 추출물인 LES가 DNCB에 의해 인위적으로 접촉피부염을 유발시킨 랫드의 피부 회복능에 미치는 효과를 조사하였다. 알레르기 접촉피부염이 유발된 피부에 LES 17.6 mg/ml를 3-5회 도포하여 피부 표면을 조직학적 분석하여 본 결과 각질이 제거된 깨끗하고 매끄러운 상태로 되었으며, 비후화된 표피층의 두께도 점차 정상군과 같은 두께로 회복시키는 효과를 나타냈다. 또한 LES는 체내 지질함량을 낮춰 정상수준으로 회복시키는 지질함량의 개선에 효과적으로 작용하고 있으며, 혈장 내 IgE의 수준도 정상군 수준으로 감소시키고 있으며, LES에 의해 염증 반응과 관련된 iNOS와 COX-2의 활성도 감소되어 피부의 과민반응 해소에 관여하는 것으로 생각된다. LES에 포함된 주 성분 중 하나인 lunasin은 세포 침투와 함께 핵 내로 이동하는 것으로 확인되어 전체 유전자 또는 일부 유전자의 발현을 조절하는 데 관여할 것으로 추측된다. 이러한 결과로 보아 LES는 알레르기성 접촉피부염에 매우 효과적인 치료 효과를 나타내고 있기 때문에 기존의 알레르기성 접촉피부염 치료제를 대체할 수 있는 제제로서 응용이 기대된다.

In this study, we evaluated whether Lunasin Enriched Soybean (LES) suppressed 1-chloro-2,4-dinitrochlorobenzene (DNCB) induced allergic contact dermatitis in vivo. In the group treated with DNCB alone, erythema and hemorrhage in the skin were observed, while the application of LES after DNCB treatment modulated erythema and hemorrhage similar to the untreated group. In histopathological analysis of the skin, DNCB-treated groups showed increased skin thickness compared to the untreated groups, while the treatment of LES dose-dependently modulated skin thickness. Also, hypertrophy and hyperkeratosis of the epidermis, intracellular edema and infiltration of inflammatory cells were observed in the group treated with DNCB alone, while treatment of LES inhibited these inflammatory changes. DNCB also induced a significant up-regulation of iNOS and COX-2 expression, as compared to the untreated groups. However, the treatment of LES significantly decreased the down-regulation of iNOS and COX-2 expression, as compared to DNCB-treated groups. Also, LES reduced the IgE level in DNCB-stimulated groups. And lunasin from LES translocated the nucleus of the cells in the skin of Sprague-Dawley rats. Taken together, it is thought that LES has the potential to be used as an alternative treatment for allergic contact dermatitis.

키워드

참고문헌

  1. Afanasieva, T. N. and N. P. Lebkova. 1987. Effect of Eleutherococcus on the subcellular structures of the heart in experimental myocardial infarct. Biull. Eksp. Biol. Med. 103, 212-215.
  2. Ban, M. and D. Hettich. 2001. Relationship between IgE positive cell numbers and serum total IgE levels in mice treated with trimellitic anhydride and dinitrochlorobenzene. Toxicol. Lett. 118, 129-137. https://doi.org/10.1016/S0378-4274(00)00280-0
  3. Choi, K. U. and D. M. Paek. 1995. Asthma and air pollution in korea. Korean J. Epidemiol. 17, 64-75.
  4. Cichocki, M., J. Blumczynska, and W. Baer-Dubowska. 2010. Naturally occurring phenolic acids inhibit 12-O-tetradecanoylphorbol- 13-acetate induced NF-$\kappa$B, iNOS and COX-2 activation in mouse epidermis. Toxicology 268, 118-124. https://doi.org/10.1016/j.tox.2009.12.013
  5. De Lumen, B. O. 2005. Lunasin: a cancer-preventive soy peptide. Nutr. Rev. 63, 16-21. https://doi.org/10.1111/j.1753-4887.2005.tb00106.x
  6. Galvez, A. F. and B. O. de Lumen. 1999. A soybean cDNA encoding a chromatin-binding peptide inhibits mitosis of mammalian cells. Nat. Biotechnol. 17, 495-500. https://doi.org/10.1038/8676
  7. Galvez, A. F., N. Chen, J. Macasieb, and B. O. de Lumen. 2001. Chemopreventive property of a soybean peptide (lunasin) that binds to deacetylated histones and inhibits acetylation. Cancer Res. 61, 7473-7478.
  8. Ishizaka, K. 1984. Regulation of IgE synthesis. Ann. Rev. Immunol. 2, 159-182. https://doi.org/10.1146/annurev.iy.02.040184.001111
  9. Kim, H. H. and D. H. Kim. 2001. The effect of yunkyopaedosangamibang on allergic contact dermatitis. J. Institute Oriental Medicine of Semyung University 3, 67-80.
  10. Kim, M. H., M. C. Kim, J. S. Park, J. W. Kim, and J. O. Lee. 2001. The antioxidative effects of the water-soluble extracts of plants used as tea materials. Korean J. Food Sci. Technol. 33, 12-18.
  11. Kim, N. K., M. H. Kim, C. S. Yoon, and S. W. Choi. 2006. Studies on the anti-inflammatory activity of Paulownia coreana uyeki leaf extract. J. Soc. Cosmet. Scientists Korea 32, 241-247.
  12. Kim, Y. H. and Y. S. Park. 2006. Effect of Acanthopanacis cortex water extract on antioxidative activity, lipid profile and epidermal thickness in DNCB-induced allergic contact dermatitis animal model. Korean J. Food Sci. Technol. 38, 668-673.
  13. Kwon, O. S., J. T. Kim, I. S. Park, S. H. Ahn, H. P. Lee, H. H. Kim, and Y. H. Gang. 1999. The effect of yunkyopaedosangamibang on allergic contact dermatitis. J. Institute Oriental Medicine of Semyung University 8, 77-91.
  14. Lam, Y., A. F. Galvez, and B. O. de Lumen. 2003. Lunasin suppresses E1A-mediated transformation of mammalian cells but does not inhibit growth of immortalized and established cancer cell lines. Nutr. Cancer 47, 88-94. https://doi.org/10.1207/s15327914nc4701_11
  15. Lee, Y. S., S. H. Jung, S. S. Lim, J. Ji, S. H. Lee, and K. H. Shin. 2001. Effect of the water extract from the stem bark of Acanthopanax senticosus on hyperlipidemia in rats. Korean J. Pharmacogn. 32, 103-107.
  16. Matsuda, H., N. Watanabe, G. P. Geba, J. Sperl, M. Tsudzuki, J. Hiroi, M. Matsumoto, H. Ushio, S. Saito, P. W. Askenase, and C. Ra. 1997. Development of atopic dermatitis-like skin lesion with IgE hyperproduction in NC/Nga mice. Int. Immunol. 9, 461-466. https://doi.org/10.1093/intimm/9.3.461
  17. McCartney-Francis, N., J. B. Allen, D. E. Mizel, J. E. Albina, Q. W. Xie, C. F. Nathan, and S. M. Wahl. 1993. Suppression of arthritis by an inhibitor of nitric oxide synthase. J. Exp. Med. 178, 749-754. https://doi.org/10.1084/jem.178.2.749
  18. Miyasaka, N. and Y. Hirata. 1997. Nitric oxide and inflammatory arthritides. Life Sci. 61, 2073-2081. https://doi.org/10.1016/S0024-3205(97)00585-7
  19. Needleman, P. and P. C. Isakson. 1997. The discovery and function of COX-2. J. Rheumatol. Suppl. 49, 6-8.
  20. Park, I. Y., T. S. Park, S. W. Oh, J. H. Woo, H. E. Jin, and H. R. Park. 2002. The effect of Eleutherococcus senticosus on human fatigue factors and oxidant-antioxidant system during maximal and submaximal exercise. Korean J. Physic. Edu. 41, 305-315.
  21. Park, J. H., S. Y. Kim, B. O. de Lumen, K. J. Lee, and H. J. Jeong. 2002. Identification and bioavailability of a chromatin- binding peptide (lunasin) from korean soybean. J. Plant Biol. 45, 96-101. https://doi.org/10.1007/BF03030290
  22. Sator, P. G., J. B. Schmidt, and H. Honigsmann. 2003. Comparison of epidermal hydration and skin surface lipids in healthy individuals and in patients with atopic dermatitis. J. Am. Acad. Dermatol. 48, 352-358. https://doi.org/10.1067/mjd.2003.105
  23. Stuehr, D. J. and M. A. Marletta. 1985. Mammalian nitrate biosynthesis: mouse macrophages produce nitrite and nitrate in response to Escherichia coli lipopolysaccharide. Proc. Natl. Acad. Sci. USA 82, 7738-7742. https://doi.org/10.1073/pnas.82.22.7738
  24. Tasaka, K. 1986. Anti-allergic drugs. Drug of Today 22, 101-133.
  25. Vestergaard, C., H. Yoneyama, M. Murai, K. Nakamura, K. Tamaki, Y. Terashima, T. Imai, O. Yoshie, T. Irimura, H. Mizutani, and K. Matsushima. 1999. Overproduction of Th2-specific chemokines in NC/Nga mice exhibiting atopic dermatitis like lesions. J. Clin. Invest. 104, 1097-1105. https://doi.org/10.1172/JCI7613
  26. Weisz, A., L. Cicatiello, and H. Esumi. 1996. Regulation of the mouse inducible-type nitric oxide synthase gene promoter by interferon-gamma, bacterical lipopolysaccharide and NG-monomethyl-L-arginine. Biochem. J. 316, 209-215.

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