DOI QR코드

DOI QR Code

Effects of Different Additives on Fermentation Characteristics and Protein Degradation of Green Tea Grounds Silage

  • Wang, R.R. (China Agricultural University) ;
  • Wang, H.L. (China Agricultural University) ;
  • Liu, X. (China Agricultural University) ;
  • Xu, C.C. (China Agricultural University)
  • Received : 2010.09.29
  • Accepted : 2010.12.29
  • Published : 2011.05.01

Abstract

This study evaluated the fermentation characteristics and protein degradation dynamics of wet green tea grounds (WGTG) silage. The WGTG was ensiled with distilled water (control), or lactic acid bacteria (LAB), enzyme (E), formic acid (FA) and formaldehyde (FD) prior to ensiling. Three bag silos for each treatment were randomly opened at 0, 3, 7, 14, 28 and 60 days after anaerobic storage. For all the treatments, except for FA, there was a rapid decline in pH during the first 7 days of ensiling. LAB treatment had higher lactic acid content, lower ammonia-N ($NH_3$-N) and free-amino nitrogen (FAA-N) contents than other treatments (p<0.05). E treatment had higher lactic acid, water-soluble carbohydrates (WSC) and non-protein nitrogen (NPN) content than the control (p<0.05). FA treatment had higher $NH_3$-N and FAA-N content than the control (p<0.05). FD treatment had lower NPN and FAA-N content than the control, but it did not significantly inhibit the protein degradation when compared to LAB treatment (p>0.05). Results indicate that LAB treatment had the best effect on the fermentation characteristics and protein degradation of WGTG silage.

Keywords

References

  1. AOAC. 1990. Official Methods of Analysis. 15th ed. Association of Official Analytical Chemists, Arlington, Virginia.
  2. Barry, T. N., J. E. Cook, R. J. Wilkins and D. E. Beever. 1978. The influence of formic acid and formaldehyde and type of harvesting machine on the utilization of silage: 2. Changes in amino acid composition during ensiling and their influence on nutritive value. J. Agric. Sci. Cambridge. 91:701-715. https://doi.org/10.1017/S002185960006010X
  3. Broderick, G. A. and J. H. Kang. 1980. Automated simultaneous determination of ammonia and total amino acid in ruminal fluid and in vitro media. J. Dairy Sci. 63:64-75. https://doi.org/10.3168/jds.S0022-0302(80)82888-8
  4. Buchanan-Smith, J. G. 1978. Metabolism of silage non-protein nitrogen in the rumen. In: Proceedings of the 5th Silage Conference. Hannah Research Institute, Ayr, Scotland, UK. pp. 30-31.
  5. Cai, Y., Y. Benno, M. Ogawa, S. Ohmomo, S. Kumai and T. Nakase. 1998. Influence of Lactobacillus spp. from an inoculant and of Weissella and Leuconostoc spp. from forage crops on silage fermentation. Appl. Environ. Microbiol. 64:2982-2987.
  6. Fairbairn, R., I. Alli and B. E. Baker. 1988. Proteolysis associated with the ensiling of chopped alfalfa. J. Dairy Sci. 71:152-158. https://doi.org/10.3168/jds.S0022-0302(88)79536-3
  7. Guo, X. S., W. Ding, J. Han and H. Zhou. 2008. Characterization of protein fractions and amino acids in ensiled alfalfa treated with different chemical additives. Anim. Feed Sci. Technol. 142:89-98. https://doi.org/10.1016/j.anifeedsci.2007.07.005
  8. Hassanat, F., A. F. Mustafa and P. Seguin. 2006. Chemical composition and ensiling characteristics of normal and brown midrib pearl millet harvested at two stages of development in southern Quebec. Can. J. Anim. Sci. 86:71-80.
  9. Henderson, N. 1993. Silage additives. Anim. Feed Sci. Technol. 45:35-56. https://doi.org/10.1016/0377-8401(93)90070-Z
  10. Hu, Z. 2010. Marketing prospects of china tea drink industry. Tea Times. 6:26-27 (In Chinese).
  11. Kondo, M., K. Kita and H. Yokota. 2004a. Effects of tea leaf waste of green tea, oolong tea, and black tea addition on sudangrass silage quality and in vitro gas production. J. Sci. Food. Agric. 84:721-727. https://doi.org/10.1002/jsfa.1718
  12. Kondo, M., N. Naoki, K. Kazumi and H. Yokota. 2004b. Enhanced lactic acid fermentation of silage by the addition of green tea waste. J. Sci. Food Agric. 84:728-734. https://doi.org/10.1002/jsfa.1726
  13. McDonald, P. and A. R. Henderson. 1964. Determination of watersoluble carbohydrates in grass. J. Sci. Food Agric. 15:395-398. https://doi.org/10.1002/jsfa.2740150609
  14. McDonald, P., A. R. Henderson and S. J. E. Heron. 1991. The Biochemistry of silage. 2nd ed. Chalcombe Publ., Cambrian Printers, Ltd., Merlow, Bucks, Aberystwyth, Wales, UK.
  15. Muck, R. E. 1987. Dry matter level effects on alfalfa silage quality: 1. Nitrogen transformations. Trans. ASAE 30:7-14. https://doi.org/10.13031/2013.30393
  16. Nagel, S. and G. A. Broderick. 1992. Effect of formic acid for formaldehyde treatment of alfalfa silage on nutrient utilization by dairy cows. J. Dairy Sci. 75:140-154. https://doi.org/10.3168/jds.S0022-0302(92)77748-0
  17. Nishino, N., T. Kawai and M. Kondo. 2007. Changes during ensilage in fermentation products, tea catechins, antioxidative acitivity and in vitro gas production of green tea waste stored with or without dried beet pulp. J. Sci. Food Agric. 87:1639-1644. https://doi.org/10.1002/jsfa.2842
  18. Rooke, J. A. and R. D. Hatfield. 2003. Biochemistry of ensiling. In: Silage Science and Technology (Ed. D. R. Buxton, R. E. Muck and J. H. Harrison). ASA Inc., Madison, WI, USA, pp. 95-140.
  19. SAS Institute. 1997. SAS/STAT User's Guide: Statistics. SAS Institute Inc., Cary, NC, USA.
  20. Tengerdy, R. P., Z. G. Weinberg, G. Szakacs, M. Wu, J. C. Linden, L. L. Henk and D. E. Johnson. 1991. Ensiling alfalfa with additives of lactic acid bacteria and enzymes. J. Sci. Food Agric. 55:215-228. https://doi.org/10.1002/jsfa.2740550207
  21. Van Soest, P. J., J. B. Robertson and B. A. Lewis. 1991. Methods for dietary fiber, neutral detergent fiber, and non-starch polysaccharides in relation to animal nutrition. J. Dairy Sci. 74:3583-3597. https://doi.org/10.3168/jds.S0022-0302(91)78551-2
  22. Voss, N. 1966. Amines and ammonia as products of protein decomposition in silage. In: Proceedings of 10th International Grassl Congress, Helsinki, Finland. pp. 540-546.
  23. Wang, J., J. Q. Wang, H. Zhou and T. Feng. 2009. Effects of addition of previously fermented juice prepared from alfalfa on fermentation quality and protein degradation of alfalfa silage. Anim. Feed Sci. Technol. 151:280-290. https://doi.org/10.1016/j.anifeedsci.2009.03.001
  24. Wilkinson, J. M., J. T. Huber and H. E. Henderson. 1976. Acidity and proteolysis as factors affecting the nutritive value of corn silage. J. Anim. Sci. 42:208-218.
  25. Xing, L., L. Chen and L. Han. 2009. The effect of an inoculant and enzymes on fermentation and nutritive value of sorghum straw silages. Biores. Tech. 100:488-491. https://doi.org/10.1016/j.biortech.2008.06.017
  26. Xu, C., Y. Cai, Y. Fujita, H. Kawamoto, T. Sato and N. Masuda. 2003. Chemical composition and nutritive value of tea grounds silage treated with lactic acid bacteria and acremonium cellulase. Nihon Chikusan Gakkaiho 74:355-361. (In Japanese) https://doi.org/10.2508/chikusan.74.355
  27. Xu, C., Y. Cai, N. Moriya and M. Ogawa. 2007. Nutritive value for ruminants of green tea grounds as a replacement of brewers' grains in totally mixed ration silage. Anim. Feed Sci. Technol. 138:228-238. https://doi.org/10.1016/j.anifeedsci.2006.11.014
  28. Xu, C., Y. Cai, J. Zhang, M. Fukasawa, H. Matsuyama and N. Moriya. 2008. Ensiling and subsequent ruminal degradation characteristics of barley tea grounds treated with contrasting additives. Anim. Feed Sci. Technol. 141:368-374. https://doi.org/10.1016/j.anifeedsci.2007.05.032

Cited by

  1. Fermentation Characteristics, Tannin Contents and <i>In vitro</i> Ruminal Degradation of Green Tea and Black Tea By-products Ensiled at Different Temperatures vol.27, pp.7, 2014, https://doi.org/10.5713/ajas.2013.13387
  2. Fermentation Characteristics and Lactic Acid Bacteria Succession of Total Mixed Ration Silages Formulated with Peach Pomace vol.28, pp.4, 2015, https://doi.org/10.5713/ajas.14.0508
  3. Effects of graded levels of liquid brewer's yeast on chemical composition and fermentation quality in cassava pulp and rice straw-based total mixed ration silage vol.88, pp.4, 2016, https://doi.org/10.1111/asj.12682
  4. Utilization of tea grounds as feedstuff for ruminant vol.4, pp.1, 2013, https://doi.org/10.1186/2049-1891-4-54
  5. Effects of Ensiling Fodder Ramie (Boehmeria nivea L.) with Lactic Acid Bacteria and Molasses on Fermentation Quality vol.524, pp.None, 2011, https://doi.org/10.4028/www.scientific.net/amr.524-527.2167
  6. Solid-State Anaerobic Microbial Ensilage: A Combined Wet Storage and Pretreatment Method for the Bioconversion of Lignocellulosic Biomass vol.11, pp.7, 2011, https://doi.org/10.1007/s12649-019-00684-z