YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Inhibitory Effect of Triticum aestivum Ethanol Extract on Lipid Accumulation in 3T3-L1 Preadipocytes

3T3-L1 세포에서 소맥엽 에탄올추출물의 지질생성 억제효과

  • Lee, Sun-Hee (Dept. of Oriental Pharmacy, College of Pharmacy, Wonkwang University and Wonkwang-Oriental Medicines Research Institute) ;
  • Xin, Mingjie (Dept. of Oriental Pharmacy, College of Pharmacy, Wonkwang University and Wonkwang-Oriental Medicines Research Institute) ;
  • Luyen, Bui Thi Thuy (College of Pharmacy, Chungnam National University) ;
  • Cha, Ji-Yun (Dept. of Oriental Pharmacy, College of Pharmacy, Wonkwang University and Wonkwang-Oriental Medicines Research Institute) ;
  • Im, Ji-Young (Dept. of Oriental Pharmacy, College of Pharmacy, Wonkwang University and Wonkwang-Oriental Medicines Research Institute) ;
  • Kwon, Se-Uk (Dept. of Oriental Pharmacy, College of Pharmacy, Wonkwang University and Wonkwang-Oriental Medicines Research Institute) ;
  • Lim, Sung-Won (Dept. of Immunology and Institute of Medical Science, Chonbuk National University Medical School) ;
  • Suh, Joo-Won (Division of Bioscience and Bioinformatics, College of Natural Science and Research Center for Bio Food and Drug Materials, Myongji University) ;
  • Kim, Young-Ho (College of Pharmacy, Chungnam National University) ;
  • Kim, Dae-Ki (Dept. of Immunology and Institute of Medical Science, Chonbuk National University Medical School) ;
  • Lee, Young-Mi (Dept. of Oriental Pharmacy, College of Pharmacy, Wonkwang University and Wonkwang-Oriental Medicines Research Institute)
  • 이선희 (원광대학교 약학대학 한약학과 및 원광한약연구소) ;
  • 신명걸 (원광대학교 약학대학 한약학과 및 원광한약연구소) ;
  • ;
  • 차지윤 (원광대학교 약학대학 한약학과 및 원광한약연구소) ;
  • 임지영 (원광대학교 약학대학 한약학과 및 원광한약연구소) ;
  • 권세욱 (원광대학교 약학대학 한약학과 및 원광한약연구소) ;
  • 임성원 (전북대학교 의과대학 및 의과학연구소) ;
  • 서주원 (명지대학교 자연과학대학 생명과학정보학부 및 농생명바이오식의약소재개발사업단) ;
  • 김영호 (충남대학교 약학대학 약학과) ;
  • 김대기 (전북대학교 의과대학 및 의과학연구소) ;
  • 이영미 (원광대학교 약학대학 한약학과 및 원광한약연구소)
  • Received : 2011.10.29
  • Accepted : 2011.11.11
  • Published : 2011.12.31
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Abstract

Non-alcoholic fatty liver disease is known to be frequently associated with obesity and type 2 diabetes. We examined the effects of EtOH extracts from Triticum aestivum on lipid accumulation during the differentiation of 3T3-L1 preadipocytes to screening the candidate materials in preventing non-alcoholic fatty liver disease. The lipid level in adipocytes was determined by Oil Red O staining. The treatment of 50% ethanol, but not water and 100% ethanol extracts, from Triticum aestivum at concentration of 0.5 $mg/ml$ inhibited lipid accumulation in 3T3-L1 cells, revealing no cell toxicity. Thus, the fractions of $CH_2Cl_2$, EtOAc and BuOH were separated from 50% EtOH extract to characterize anti-adipogenic effect. The $CH_2Cl_2$ fraction at concentration of $50{\mu}g/ml$ effectively inhibited the lipid accumulation in the adipocytes compared to those of EtOAc and BuOH at concentration of $50{\mu}g/ml$. The intracellular triglyceride accumulation also was significantly reduced by treatment of $CH_2Cl_2$ fraction in concentration-dependent manner. Western blot analysis showed that the $CH_2Cl_2$ fraction attenuated the intracelluar level of fatty acid synthase(FAS) accompanied by attenuated expression of Peroxidase proliferator-activated receptor ${\gamma}$ ($PPAR{\gamma}$) adipogenic transcription factor. These results suggest that $CH_2Cl_2$ fraction from 50% EtOH extract of Triticum aestivum may has the potent anti-adipogenic effects by inhibiting the transactivation of $PPAR{\gamma}$.

Keywords

References

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