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Antioxidative Effect of Suaeda japonica Ethanol Extract and Solvent Partitioned Fractions

칠면초 추출물의 항산화 효과

  • Received : 2011.04.13
  • Accepted : 2011.05.13
  • Published : 2011.06.25

Abstract

We studied the antioxidant activity of Suaedajaponica, found in Korean mud flats. A 70% ethanol extraction yielded 9.74%. The 70% ethanol extract was further fractionated with hexane, chloroform, ethyl acetate, butanol, and water. The water fraction resulted in the highest extraction yields, but the ethyl acetate fraction resulted in the highest total phenol content and also total flavonoid content. The antioxidative activity of the fractions was evaluated using several methods and compared to commercial antioxidants. The scavenging activities of the hydroxyl radical, hydrogen radical, hydrogen peroxide, and xanthine oxidase of the ethyl acetate fraction were equivalent to that of BHA. The scavenging activities of the hydroxyl radical, hydrogen peroxide, and xanthine oxidase of the ethanol extracts were approximately 80~90% of that of BHA.

본 연구는 염생식물이라는 특성으로 인해 갯벌이 발달한 우리나라에서 쉽게 접할 수 있는 칠면초의 항산화활성을 검토하고자 이루어졌다. 70% ethanol로 추출한 추출수율은 9.74%였으며 이를 hexane, chloroform, ethyl acetate, butanol, water로 순차 분획한 수율은 water 층이 원물대비 8.73%로 가장 높은 수율을 보였고 대체적으로 극성이 높을수록 높은 분획 수율을 나타내었다. 하지만 분획물간 총 페놀과 총 플라보노이드 함량 측정 결과 ethyl acetate 분획물의 에서 가장 높게 측정되었다. Ethyl acetate 분획물의 항산화력을 hydroxyl radical 소거능, hydrogen peroxide 소거능, xanthine oxidase 소거능 등을 측정하여 합성항산화제인 BHA와 비교한 결과 BHA의 80~90% 가량의 활성을 보여주었다.

Keywords

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