Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
권호 표지
DOI QR코드

DOI QR Code

Oral Toxicity Study on the 90-day Repeated-dose of 50 kGy Irradiated Methanol Extract Powder of Red Ginseng

50 kGy 감마선 조사된 홍삼 메탄올 추출물의 90일 반복 투여 독성시험

  • Jeon, Young-Eun (Dept. of Food Science and Nutrition & The Korean Institute of Nutrition, Hallym University) ;
  • Kim, Jin-Kyung (Dept. of Medical Life Science, Catholic University of Daegu) ;
  • Yin, Xing-Fu (Dept. of Food Science and Nutrition & The Korean Institute of Nutrition, Hallym University) ;
  • Lee, Ju-Woon (Division of Radiation Application Research, Korea Atomic Energy Research Institute) ;
  • Nam, Jin-Sik (Dept. of Food and Nutrition, Suwon Women's College) ;
  • Kang, Il-Jun (Dept. of Food Science and Nutrition & The Korean Institute of Nutrition, Hallym University)
  • 전영은 (한림대학교 식품영양학과.한국영양연구소) ;
  • 김진경 (대구가톨릭대학교 의생명과학과) ;
  • 윤성복 (한림대학교 식품영양학과.한국영양연구소) ;
  • 이주운 (한국원자력연구원) ;
  • 남진식 (수원여자대학 식품영양과) ;
  • 강일준 (한림대학교 식품영양학과.한국영양연구소)
  • Received : 2010.10.14
  • Accepted : 2010.11.07
  • Published : 2011.06.25
Download PDF
⟨ Previous Next ⟩

Abstract

This study was to investigate 90-day repeated-dose toxicities of 50 kGy irradiated methanol extract powder of red ginseng in ICR mice. The test materials (methanol extract powder of red ginseng with or without 50 kGy irradiation) were administered by gavage to male and female ICR mice at dose levels of 0, 125, 250 and 500 mg/kg/day for 90 days. In the results, no abnormality was observed in mortality, clinical findings, body weight changes, food consumptions, opthalmoscopic findings, necropsy findings and histopathological findings. Although the minor changes in blood and biochemical parameters were observed, they were not dose dependent and not affected by gamma irradiation. In conclusion, 90-day repeated oral dose of the methanol extract powder of red ginseng and 50 kGy irradiated methanol extract powder of red ginseng to ICR mice did not cause apparent toxicological change at the dose of 125, 250 and 500 mg/kg body weight.

본 연구에서는 방사선을 조사한 홍삼 메탄올 추출물의 안전성을 검토하고자 식품의약안전청의 의약품 등의 독성시험기준에 따라 ICR계열의 암수 마우스에 시험물질을 0, 125, 250 및 500 mg/kg/day의 용량으로 90일간 반복경구 투여한 후, 사망률, 일반증상, 체중변화, 혈액 및 혈액생화학적 변화, 부검소견, 조직학적인 변화를 관찰하였다. 시험기간 중 암수 모든 군에서 시험물질 투여에 기인한 일반적인 증상변화는 관찰되지 않았고, 시험물질의 반복 투여로 인한 사망례 역시 관찰되지 않았다. 시험물질의 투여에 기인한 유의적인 체중 감소 또한 나타나지 않았으며, 상기 이외의 육안적인 부검소견에서도 시험물질 투여에 기인한 어떠한 이상소견도 발견되지 않았다. 혈액학적 분석 결과 일부 시험물질 투여군에서 총 백혈구 수 등의 수치가 유의적인 변화를 보였으나, 정상범위 내에서의 변화로 방사선조사에 의해 야기된 독성은 아니었다. 간장과 신장의 조직학적인 관찰에서 시험물질 투여에 의한 변화는 관찰되지 않았다. 따라서 방사선 구조변환 홍삼 추출물을 3개월간 ICR 마우스에 섭취시킨 경우, 시험한 최고 농도인 500 mg/kg/day에서는 독성이 없는 것으로 판명되었다.

Keywords

References

  1. Choi KJ. 1991. Constituent and quality control of ginseng. Korean J Ginseng Sci 15: 247-256.
  2. Garriques S. 1854. On panaquilon, a new vegetable substance. Ann Chem Pharm 90: 231-234. https://doi.org/10.1002/jlac.18540900216
  3. Brekhman II. 1957. Panax ginseng. Media Leningrad 182: 55-58.
  4. Shibata S, Tanaka O, Ando T, Sado M, Tsushima S, Ohsawa T. 1966. Chemical studies on oriental plants drugs. XIV. Protopanaxadiol, a genuine sapogenin of ginseng saponins. Chem Pharm Bull 14: 595-600. https://doi.org/10.1248/cpb.14.595
  5. Nam KY. 2002. Clinical applications and efficacy of Korea ginseng. J Ginseng Res 26: 111-131. https://doi.org/10.5142/JGR.2002.26.3.111
  6. Park JD. 1996. Recent studies on the chemical constituents of Korea ginseng. Korean J Ginseng Sci 20: 389-415.
  7. Chepurnov SA, Chepurnova NE, Park JK, Buzinova EV, Lubimov II, Kabanova NP, Nam KY. 1994. The central effects of saponin components and polysaccharides fraction from Korea red ginseng. Korean J Ginseng Sci 18: 165-174.
  8. Yun TK, Lee YS, Lee YH, Yun HY. 2001. Cancer chemopreventive compounds of red ginseng produced from Panax ginseng C.A. Meyer. J Ginseng Res 25: 107-111.
  9. Keum YS, Park KK, Lee JM, Chun KS, Park JH, Lee SK, Kwon H, Surh YJ. 2000. Antioxidant and anti-tumor promoting activities of the methanol extract of heat-processed ginseng. Cancer Lett 150: 41-48. https://doi.org/10.1016/S0304-3835(99)00369-9
  10. Jeon BH, Kim HS, Chang SJ. 1999. Effect of saponin and non-saponin of Panax ginseng on the blood pressure in the renovascular hypertensive rats. J Ginseng Res 23 : 81-87.
  11. Kang SY, Kim ND. 1992. The antihypertensive effect of red ginseng saponin and the endothelium-derived vascular relaxation. Korean J Ginseng Sci 16: 175-182.
  12. Kim DH, Kwak KH, Lee KJ, Kim SJ. 2004. Effects of Korea red ginseng total saponin on repeated unpredictable stress induced changes of proliferation of neural progenitor cells and BDNF mRNA expression in adult rat hippocampus. J Ginseng Res 28: 94-103. https://doi.org/10.5142/JGR.2004.28.2.094
  13. Petkov VD, Mosharrof AH. 1987. Effects of standardized ginseng extract on learning, memory and physical capabilities. Am J Chin Med 15: 19-29. https://doi.org/10.1142/S0192415X87000047
  14. Lee JH, Park HJ. 1995. Effects of lipophilic fraction from Korean red ginseng on platelet aggregation and blood coagulation in rats fed with corn oil and beeftallow diet. Korean J Ginseng Sci 19: 206-211.
  15. Jung KY, Kim DS, Oh SR, Lee IS, Lee JJ, Park JD, Kim SI, Lee HK. 1998. Platelet activating factor antagonist activity of ginsenosides. Biol Pharm Bull 21: 79-80. https://doi.org/10.1248/bpb.21.79
  16. Bae KC, Kim SH. 1998. Antioxidant effects of Korea ginseng radix, Korea red radix and total saponin. Korean J Oriental Medical Pathology 12: 72-81.
  17. Ananthaswamy HN, Vakil UK, Sreenvasan A. 1970. Effect of gamma radiation on wheat starch and its components. J Food Sci 35: 795-798. https://doi.org/10.1111/j.1365-2621.1970.tb01997.x
  18. Charlesby A. 1981. Crosslinking and degradation of polymers. Radiat Phys Chem 18: 59-66. https://doi.org/10.1016/0146-5724(81)90064-9
  19. Cho M, Kim BY, Rhim JH. 2003. Degradation of alginate solution and powder by gamma irradiation. Food Eng Prog 7: 141-145.
  20. Byun EH, Kim JH, Sung NY, Choi JI, Lim ST, Kim KH, Yook HS, Byun MW, Lee JW. 2008. Effects of gamma irradiation on the physical and structural properties of $\beta$-glucan. Radiat Phys Chem 77: 781-786. https://doi.org/10.1016/j.radphyschem.2007.12.008
  21. Kim JK, Sung NY, Srinivasan P, Choi J, Kim SK, Oh JM, Kim JH, Song BS, Park HJ, Byun MW, Lee JW. 2008. Effect of gamma irradiated hyaluronic acid on acetaminophen induced acute hepatotoxicity. Chem Biol Interact 172: 141- 153. https://doi.org/10.1016/j.cbi.2007.11.015
  22. Kim MS, Kwon B, Park MS, Ji GE. 2008. Isolation of ginsenoside Rh1 and compound K from fermented ginseng and efficasy assessment on systemic anaphylactic shock. Food Sci Biotechnol 17: 805-808.
  23. Wolford ST, Schroer RA, Gohs FX, Gallo PP, Brodec M, Falk HB, Ruhren FR. 1986. Reference range data base for serum chemistry and hematology values in laboratory animals. J Toxicol Environ Health 18: 161-188. https://doi.org/10.1080/15287398609530859

Cited by

  1. Changes in the Components of Red Ginseng after Irradiation and the Korean Consumer's Perception of Irradiated Food vol.45, pp.1, 2011, https://doi.org/10.14407/jrpr.2020.45.1.26