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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Effects of novel chalcone derivatives on α-glucosidase, dipeptidyl peptidase-4, and adipocyte differentiation in vitro

  • Bak, Eun-Jung (Oral Cancer Research Institute, Yonsei University College of Dentistry) ;
  • Park, Hong-Gyu (Atgc Biotechnology Co., Ltd.) ;
  • Lee, Choong-Hwan (ATGen Co., Ltd.) ;
  • Lee, Tong-Il (ATGen Co., Ltd.) ;
  • Woo, Gye-Hyeong (Department of Clinical Laboratory Science, Semyung University) ;
  • Na, Young-Hwa (College of Pharmacy, CHA University) ;
  • Yoo, Yun-Jung (Department of Oral Biology, BK21 Project, Oral Science Research Center, Research Center for Orofacial Hard Tissue Regeneration, Yonsei University College of Dentistry) ;
  • Cha, Jeong-Heon (Oral Cancer Research Institute, Yonsei University College of Dentistry)
  • Received : 2011.04.15
  • Accepted : 2011.04.19
  • Published : 2011.06.30
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Abstract

Chana series are new chalcone derivatives. To evaluate the possibility of Chana series as therapeutic agents of type 2 diabetes, the inhibitory effects of Chana series on the activities of ${\alpha}$-glucosidase and DPP-4 were investigated using in vitro enzyme assays, and their effects on adipocyte differentiation were investigated in C3H10T1/2 cells. Chana 1 and Chana 7 among the Chana series showed significant inhibition of ${\alpha}$-glucosidase activity. In DPP-4 enzyme assay, Chana 1 exhibited the highest inhibitory activity while Chana 7 did not. In MTT assay, Chana 1 did not show significant cytotoxicity up to a concentration of $250{\mu}M$, whereas cytotoxicity was observed with Chana 7 at a concentration of $300{\mu}M$. In addition, Chana 1 induced adipocyte differentiation. Therefore, Chana 1 showed inhibitory effects on ${\alpha}$-glucosidase and DPP-4 as well as a stimulatory effect on adipocyte differentiation, suggesting that Chana 1 may be a potential beneficial agent for the treatment of type 2 diabetes.

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References

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