Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Nutritional Properties and Biological Activities of Artemisia annua L.

개똥쑥의 영양적 특성 및 생리활성

  • Ryu, Ji-Hyun (Dept. of Food Science and Nutrition, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Kim, Ra-Jeong (Dept. of Food Science and Nutrition, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Lee, Soo-Jung (Dept. of Food Science and Nutrition, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Kim, In-Soo (Dept. of Food Science and Nutrition, Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Lee, Hyun-Ju (Division of Food Science, International University of Korea) ;
  • Sung, Nak-Ju (Dept. of Food Science and Nutrition, Institute of Agriculture and Life Science, Gyeongsang National University)
  • 류지현 (경상대학교 식품영양학과.농업생명과학연구원) ;
  • 김라정 (경상대학교 식품영양학과.농업생명과학연구원) ;
  • 이수정 (경상대학교 식품영양학과.농업생명과학연구원) ;
  • 김인수 (경상대학교 식품영양학과.농업생명과학연구원) ;
  • 이현주 (한국국제대학교 식품과학부) ;
  • 성낙주 (경상대학교 식품영양학과.농업생명과학연구원)
  • Received : 2010.11.18
  • Accepted : 2011.01.26
  • Published : 2011.02.28
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Abstract

The nutritional properties and biological activities of leaves and stems of Gaeddongssuk (Artemisia annua L.) were investigated. Contents of moisture, crude lipid and crude protein were significantly higher in the leaves, and then ash, crude fiber and mineral were significantly higher in the stems. Contents of total phenols and flavonoids of leaves were about 2 fold higher than those of stems. Antioxidant activity was significantly increased in a dose-dependent manner; also, water and ethanol extracts of leaves were stronger than those of stems. Especially, DPPH radical scavenging activity, reducing power and tyrosinase inhibition activity were significantly higher in leaves extracts than stems extracts of Gaeddongssuk. But, ${\alpha}$-glucosidase inhibition activity was higher in stem than its leaves extract. In MTT assay by human breast adenocarcinoma cell line MCF-7 and MDA-MB-231, ethanol extracts of leaves showed the highest anticancer activity; the rates of growth inhibition were 76.26% and 52.59% on MCF-7 and MDA-MB-231 cells, at the concentration of $250\;{\mu}g$/mL, respectively. In conclusion, biological activities of extracts from Gaeddongssuk were dependent on the fiber, phenolic and flavonoid content.

개똥쑥(Artemisia annua L.)의 기능성을 밝히고 식품으로써 이용성을 증대시키기 위하여 개똥쑥 잎과 줄기의 영양 성분 및 생리활성을 측정하였다. 수분, 조지방 및 조단백질 함량은 잎에서 높았으나 회분, 조섬유 및 무기물 함량은 줄기에서 유의적으로 높았다. 총 페놀과 플라보노이드 함량은 잎이 줄기에 비해 약 2배 정도 높았다. 추출물의 농도를 달리하여 항산화능을 측정한 결과 농도에 의존적으로 활성이 증가하였으며, 잎은 줄기에 비해 활성이 높았다. 특히, DPPH 라디칼 소거능, 환원력 및 tyrosinase 저해활성은 모든 농도에서 잎이 줄기에 비해 유의적으로 높았으나, ${\alpha}$-glucosidase 저해활성은 줄기 추출물이 더 높았다. 인체 유방암 세포인 MCF-7 및 MDA-MB-231에 대한 증식억제 활성은 줄기보다 잎 추출물에서, 물보다 에탄올 추출물에서 유의적으로 활성이 높았다. 특히, 잎 에탄올 추출물의 MCF-7 및 MDAMB-231 세포에 대한 증식억제 활성은 $250\;{\mu}g$/mL에서 각각 76.26% 및 52.59%로 추출물 중 가장 높은 활성을 보였다. 이상의 결과, 개똥쑥 추출물의 생리활성은 시료 중의 섬유소, 페놀 및 플라보노이드 함량에 의존적인 것으로 생각된다.

Keywords

References

  1. Lee CB. 1997. Korea botanical book. Jin Myung Publication Co., Seoul, Korea. p 292.
  2. Lee SD. Park HH, Kim DW, Bang BH. 2000. Bioactive constituents and utilities of Artemisia sp. as medical herb and foodstuff. Korean J Food & Nutr 13: 490-505.
  3. Sim YJ, Han YS, Chun HJ. 1992. Studies on the nutritional components of mugwort Artemisia mongolica Fischer. Korean J Food Sci Technol 24: 49-53.
  4. Choi BB, Lee HJ, Bang SK. 2005. Studies on the volatile components and biochemical characterizations of Artemisia princeps and A. argyi. Korean J Food & Nutr 18: 334-340.
  5. Lee GD, Kim JS, Bae JO, Yoon HS. 1992. Antioxidative effectiveness of water extract and ether extract in wormwood (Artemisia montana Pampan). J Korean Soc Food Nutr 21: 17-22.
  6. Hong JH, Jeon JL, Lee JH, Lee IS. 2007. Antioxidative properties of Artemisia princeps Pamp. J Korean Soc Food Sci Nutr 36: 657-662. https://doi.org/10.3746/jkfn.2007.36.6.657
  7. Jung MJ, Yin Y, Heo SI, Wang MH. 2008. Antioxidant and anticancer activities of extract from Artemisia capollaries. Kor J Pharmacogn 39: 194-198.
  8. Lee HO, Han KY, Han DM. 1999. Antibacterial and antifungal effect by Artemisia lavandulaefolia essential oil. Korean J Food & Nutr 12: 559-563.
  9. Lee SJ, Chung HY, Lee IK, Yoo ID. 1999. Isolation and identification of flavonoid from ethanol extracts of Artemisia vulgaris and their antioxidant activity. Korean J Food Sci Technol 31: 815-822.
  10. Wang XJ, Sun H, Choi SU. 1997. Quantitative analysis of 6,7-dimethylesculetin and capillarisine in Artemisia capillares Thunb. and prescriptions containing the crude drug. Zhongguo Zhong Yao Za Zhi 19: 667-670.
  11. Klayman DL. 1985. Qinghaosa (Artemisia): An antimalarial drug from China. Science 228: 1049-1055. https://doi.org/10.1126/science.3887571
  12. Fabien J, Veronique M, Jean MB, Mechel D, Josette V. 2002. Antibacterial and antioxidant activities of Artemisia annua essential oil. Fitoterapia 73: 532-535. https://doi.org/10.1016/S0367-326X(02)00175-2
  13. Kim KB, Yoo KH, Park HY, Jeong JM. 2006. Anti-oxidatives activities of commercial edible plant extracts distributed in Korea. J Korean Soc Appl Biol Chem 49: 328-333.
  14. Kim HC, Kil BS, Lee YH. 2001. The antifungal activity of chemical substances from Artemisia annua. Korean J Ecol 24: 137-140.
  15. AOAC. 1995. Official methods of analysis. 16th ed. Patricia C, ed. Association of Official Analytical Chemists, Arlington, VA, USA. Vol 2, Ch 26, p 36.
  16. Kang YH, Park YK, Oh SR, Moon KD. 1995. Studies on the physiological functionality of pine needle and mugwort extracts. Korean J Food Technol 27: 978-984.
  17. Gutfinger T. 1981. Polyphenols in olive oil. J Am Oil Chem Soc 58: 966-968. https://doi.org/10.1007/BF02659771
  18. Moreno MIN, Isla MI, Sanpietro AR, Vattuone MA. 2000. Comparison of the free radical scavenging activity of propolis from several region of Argentina. J Enthropharmacol 71: 109-114. https://doi.org/10.1016/S0378-8741(99)00189-0
  19. Blois MS. 1958. Antioxidant determination by the use of a stable free radical. Nature 181: 1199-1200. https://doi.org/10.1038/1811199a0
  20. Oyaizu M. 1986. Studies on products of browning reaction: Antioxidant activities of products of browning reaction prepared from glucosamine. Japanese J Nutr 44: 307-315. https://doi.org/10.5264/eiyogakuzashi.44.307
  21. Yagi A, Kanbara T, Morinobu N. 1986. The effect of tyrosinase inhibition for aloe. Planta Med 3981: 517-519.
  22. Choe M, Kim DJ, Lee HJ, You JK, Seo DJ, Lee JH, Chung MJ. 2008. A study on the glucose regulating enzymes and antioxidant activities of water extracts from medicinal herbs. J Korean Soc Food Sci Nutr 37: 542-547. https://doi.org/10.3746/jkfn.2008.37.5.542
  23. Mosmann T. 1983. Rapid colorimetric assay for cellular growth and survival: applicaton to proliferation and cytotoxicity assays. J Immunol Meth 65: 55-63. https://doi.org/10.1016/0022-1759(83)90303-4
  24. Brisibe EA, Umoren UE, Brisibe F, Magalhaes PM, Ferreira JFS, Luthria D, Wu X, Prior RL. 2009. Nutritional characterization and antioxidant capacity of different tissues of Artemisia annua L. Food Chem 115: 1240-1246. https://doi.org/10.1016/j.foodchem.2009.01.033
  25. Lee SD, Park HH. 2001. Effect of feeding basal diet supplemented with mugwort power on the serum components in growing rat. Korean J Food & Nutr 13: 411-417.
  26. Choi SR, You DH, Kim JY, Park CB, Ryu J, Kim DH, Eun JS. 2008. Antioxidant and antimicrobial activities of Artemisia capillaris Thunberg. Korean J Medicinal Crop Sci 16: 112-117.
  27. Roh TH, Lee JC, Lee KS, Shim JS. 1994. Component comparison of Artemisia selengensis and Artemisia sp. for Artemisia selengensis utilization. Korean J Medicinal Crop Sci 2: 174-179.
  28. Lee HJ, Hwang EH, Yu HH, Song IS, Kim CM, Kim MC, Hong JH, Kim DS, Han SB, Kang KJ, Lee EJ, Chung HW. 2002. The analysis of nutrients in Artemisia capillaris Thunberg. J Korean Soc Food Sci Nutr 31: 361-366. https://doi.org/10.3746/jkfn.2002.31.3.361
  29. Kim CH. 2009. Antioxidant activity and quality characteristics of Artemisia sp. with different heat treatments. Korean J Culinary Res 15: 128-138.
  30. Jung IC. 2006. Rheological properties and sensory characteristics of white bread with added mugwort powder. J East Asian Soc Dietary Life 16: 332-343.
  31. Park CS, Kwon CJ, Choi MA, Park GS, Choi KH. 2002. Antioxidative and nitrite scavenging activities of mugwort and pine needle extracts. Korean J Food Preserv 9: 248-252.
  32. Kim EY, Baik IH, Kim JH, Kim SR, Rhyu MR. 2004. Screening of the antioxidant activity of some medicinal plants. Korean J Food Sci Technol 36: 333-338.
  33. Gordon MF. 1990. The mechanism of antioxidant action in vitro. In Food Antioxidants. Hudson BJF, ed. Elsevier Applied Science, London, UK. p 1-18.
  34. Tabart J, Kevers C, Pincemail J, Defraigne JO, Dommes J. 2009. Comparative antioxidant capacities of phenolic compounds measured by various tests. Food Chem 113: 1226-1233. https://doi.org/10.1016/j.foodchem.2008.08.013
  35. Kwak JH, Seo UK, Han YH. 2001. Inhibitory effect of mugwort extracts on tyrosinase activity. Korean J Biotechnol Bioeng 16: 220-223.
  36. Shin JY. 2001. Screening of natural products that have activities against skin-aging. Korean J Food & Nutr 14: 568-572.
  37. Hyun SH, Jung SK, Jwa MK, Song CK, Kim JH, Lim SB. 2007. Screening of antioxidants and cosmeceuticals from natural plant resources in Jeju island. Korean J Food Sci Technol 39: 200-208.
  38. Cho YJ, Bang MA. 2004. Hypoglycemic and antioxidative effects of dietary sea-tangle extracts supplementation in streptozotocin-induced diabetic rats. Korean J Nutr 37: 5-14.
  39. Sharma B, Balomajumder C, Roy P. 2008. Hypoglycemic and hypolipidemic effects of flavonoid rich extract from Eugenia jambolana seeds on streptozotocin induced diabetic rats. Food Chem Toxicol 46: 2376-2383. https://doi.org/10.1016/j.fct.2008.03.020
  40. Tastekin D, Atasever M, Adiguzel G, Keles M, Tastekin A. 2006. Hypoglycemic effect of Artemisia herba-alba in experimental hyperglycemic rats. Bull Vet Inst Pulawy 50: 235-238.
  41. Baron AD. 1998. Postprandial hyperglycemia and $\alpha$-glucosidase inhibitors. Diabetes Res Clin Pract 40: 51-55. https://doi.org/10.1016/S0168-8227(98)00043-6
  42. Lee MK, Choi GP, Ryu LH, Lee GY, Yu CY, Lee HY. 2004. Enhanced immune activity and cytotoxicity of Artemisia capillaris Thunb. extracts against human cell line. Korean J Medicinal Crop Sci 12: 36-42.
  43. Kim KN, Lee JA, Yoon WJ, Kim JY, Song GP, Park SY. 2007. The cytotoxicity of Artemisia fukudo extracts against HL-60 cells. J Korean Soc Food Sci Nutr 36: 819-824. https://doi.org/10.3746/jkfn.2007.36.7.819
  44. Singh NP, Lai H. 2001. Selective toxicity of dihydroartemisinin and holotransferin toward human breast cancer cells. Life Sci 70: 49-56. https://doi.org/10.1016/S0024-3205(01)01372-8
  45. Hwang YK, Kim DC, Hwang WI, Han YB. 1998. Inhibitory effect of Artemisia princeps Pampan. extract on growth of cancer cell lines. Korean J Nutr 31: 799-808.
  46. Park CS, Kim ML. 2006. Functional properties of mugwort extracts and quality characteristics of noodles added mugwort powder. Korean J Food Preserv 13: 161-167.

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