The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
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The anti-inflammatory effects of Huang-Lyun (Coptidis Rhizoma, CR) on injured tissue after burn elicitation

황련이 화상조직에 미치는 항산화와 항염증 및 피부재생 효과

  • Kim, Hee-Kyung (Dept. of Oriental Medical Ophthalomlogy, Otolaryngology & Dermatology College of Oriental Medicine, Dongguk University) ;
  • Hong, Seung-Ug (Dept. of Oriental Medical Ophthalomlogy, Otolaryngology & Dermatology College of Oriental Medicine, Dongguk University)
  • 김희경 (동국대학교 한의과대학 안이비인후피부과학교실) ;
  • 홍승욱 (동국대학교 한의과대학 안이비인후피부과학교실)
  • Received : 2010.12.22
  • Accepted : 2011.02.15
  • Published : 2011.03.30
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Abstract

Background and Objective: Coptidis Rhizoma is a medicinal herb known for its antioxidant and anti-inflammatory effect. The purpose of this study was to examine the effects of CR on the experimental burn elicitation in vitro and in vivo. Material and Methods: In order to know the antioxidant effect on skin cell of mice after burn elicitation, superoxide dismutase (SOD) activity was measured. In vitro, the RAW 264.7 macrophage cells were treated with lipopolysaccharides for experimental inflammation. iNOS mRNA expression was observed after CR-treatment. In order to know effects on the skin regeneration in the burned mice, we counted the nitric oxide (NO) in blood. We also observed the histological structure in the epidermal basal layer and the dermal section, and we studied changes of angiogenesis in the capillaries surrounding the basal layer and dermal papilla. The changes of transcription of iNOS mRNA (inducible nitric oxide synthase mRNA) and changes of NF-${\kappa}$B (nuclear factor ${\kappa}$B) p65 positive reaction were also observed to investigate the changes of the stress in the skin. Results: The results indicated that CR has significant effects on the antioxidant effect on skin cells of mice after burn elicitation by increasing SOD activity in the in vitro test. It seemed that CR decreased the amount of NF-${\kappa}$B which induced the iNOS mRNA dose-dependently and suppress activating NO and angiogenesis. Furthermore, CR facilitated the process of skin recovery after experimental burn. Conclusion: CR can be applied for burned skin via antioxidant effect and skin regeneration.

Keywords

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