Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Temperature Sensitivity of Sigma Background Is Suppressed by the Disruption of ScKNS1 in Saccharomyces cerevisiae

ScKns1 결손에 의한 Saccharomyces cerevisiae ${\Sigma}1278b$ 균주의 온도 민감성 억제 효과

  • Park, Yun-Hee (Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University) ;
  • Park, Hee-Moon (Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University)
  • 박윤희 (충남대학교 생명시스템과학대학 미생물.분자생명과학과) ;
  • 박희문 (충남대학교 생명시스템과학대학 미생물.분자생명과학과)
  • Received : 2011.06.03
  • Accepted : 2011.06.14
  • Published : 2011.06.30
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Abstract

The Saccharomyces cerevisiae S288c strain does not show haploid and diploid filamentous growth, and biofilm formation, because it has a flo8 nonsense mutation unlike ${\Sigma}1278b$ strain which has a FLO8 gene. During the heat stress experiments to investigate the role of ScKns1, LAMMER kinase in S. cerevisiae, we found that ${\Sigma}1278b$ strain revealed heat sensitivity at $37^{\circ}C$, a mild heat stress in contrast to S288c strain. We also found that the disruption of ScKns1 and the addition of sorbitol suppress heat sensitivity of ${\Sigma}1278b$ strain. These results suggest the possibility that Flo8 and ScKns1 may interact to transducer a signal for regulating heat stress through a novel signaling pathway.

Saccharomyces cerevisiae ${\Sigma}1278b$ 균주는 FLO8 유전자를 가지고 있는 반면, S288c 균주는 flo8 정지돌연변이를 포함하고 있어서 반수체의 부착 생장, 배수체의 위균사형 생장 및 생물막 형성 현상을 보이지 않는다. S. cerevisiae의 LAMMER kinase인 ScKns1의 기능을 파악하기 위한 온도 감수성 실험을 수행한 결과, S288c 균주와 달리 ${\Sigma}1278b$ 균주가 $37^{\circ}C$의 열 스트레스에 민감함을 발견하였으며, 흥미롭게도 $Sckns1{\Delta}$ 돌연변이가 이러한 감수성을 회복시킴을 확인하였다. 또한 ${\Sigma}1278b$ 균주의 열 감수성은 삼투안정제인 sorbitol의 첨가에 의해 회복되었다. 이러한 결과는 Flo8은 ScKns1이 연관된 열 스트레스를 조절하는 신호전달경로를 구성할 가능성을 제시한다.

Keywords

References

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