Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Conservation of the Epothilone-Biosynthetic Genes in Sorangium cellulosum Strains

Sorangium cellulosum 균주들의 에포틸론 생합성 유전자 보존

  • Hyun, Hye-Sook (Myxobacteria Bank, Department of Biotechnology, Hoseo University) ;
  • Youn, Jin-Kwon (Myxobacteria Bank, Department of Biotechnology, Hoseo University) ;
  • Cho, Kung-Yun (Myxobacteria Bank, Department of Biotechnology, Hoseo University)
  • 현혜숙 (호서대학교 생명공학과 점액세균은행) ;
  • 윤진권 (호서대학교 생명공학과 점액세균은행) ;
  • 조경연 (호서대학교 생명공학과 점액세균은행)
  • Received : 2011.06.13
  • Accepted : 2011.06.21
  • Published : 2011.06.30
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Abstract

The epothilone biosynthetic gene cluster (epoA~F, epoK) of Sorangium cellulosum KYC3013, an epothilone producing myxobacterium isolated in Korea, was cloned. When the amino acid sequences of the encoded proteins were compared with those from S. cellulosum SMP44, S. cellulosum So ce90, and S. cellulosum So0157-2, which were isolated in other continents or country, the proteins from different strains were 97.4-99.8% identical each other. This suggested that the epothilone-biosynthetic gene clusters are well conserved in S. cellulosum strains.

국내에서 분리된 에포틸론 생산 점액세균 Sorangium cellulosum KYC3013의 에포틸론 생합성 유전자군(epoA~F, epoK)을 클로닝하였다. 이 유전자들이 암호화하고 있는 단백질들의 아미노산 서열을 다른 대륙 또는 나라에서 분리된 S. cellulosum SMP44, S. cellulosum So ce90, S. cellulosum So0157-2의 단백질들과 비교한 결과 서로 97.4-99.8% 동일하였다. 이러한 결과는 에포틸론 생합성 유전자들이 S. cellulosum 균주들 사이에서 잘 보존되어 있음을 보여주었다.

Keywords

References

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