Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Understanding of Extracellular Fumarate Induced dctA Gene Expression Profile Using GFP Reporter

GFP 리포터를 이용한 외부 푸마르산 유도 dctA 유전자 발현 특성 파악

  • ;
  • ;
  • 김주한 (울산대학교 공과대학 생명화학공학부) ;
  • 홍순호 (울산대학교 공과대학 생명화학공학부)
  • Received : 2011.05.17
  • Accepted : 2011.06.27
  • Published : 2011.06.30
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Abstract

In Escherichia coli, DcuS/R two-component system controls fumarate import and utilization related gene expression. To understand the dynamic response of the bacterium DcuS/R two-component system with respect to fumarate concentrations, DcuS/R induced dctA promoter was integrated with GFP reporter protein. Expression monitoring study using recombinant strain showed that dctA promoter was upregulated with 1 mM of fumarate in M9 minimal medium.

본 연구에서는 외부의 푸마르산을 인식하는 DcuS/R TCS에 의하여 발현이 조절되는 dctA 유전자의 발현 특성을 GFP를 이용하여 관찰하였으며, 1 mM의 푸마르산을 감지하여 GFP를 발현 시킬 수 있음을 확인하였다. 결론적으로, 개량된 E. coli는 간단한 dctA 프로모터와 GFP의 융합을 이용하여 푸마르산을 모니터 할 수 있으며, 이것은 상승된 푸마르산 농도 조건하에서 원활히 작동함을 확인할 수 있었다.

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References

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