Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Analysis of Expression Pattern of the Limonoid UDP-glucosyltransferase Gene as an Indicator for Delayed Bitterness from the Citrus Species Endemic in Jeju

재래귤의 성숙시기별 리모노이드 쓴맛 표시자로서 limonoid UDP-glucosyltransferase 발현 분석

  • Kim, Young-Mee (Department of Medicine, Medical School, Jeju National University) ;
  • Lee, Do-Seung (Faculty of Biotechnology, College of Applied Life Sciences, Jeju National University) ;
  • Jeon, Deok-Hyoen (Faculty of Biotechnology, College of Applied Life Sciences, Jeju National University) ;
  • Song, Yeon-Woo (Faculty of Biotechnology, College of Applied Life Sciences, Jeju National University) ;
  • Lee, Dong-Sun (Faculty of Biotechnology, College of Applied Life Sciences, Jeju National University) ;
  • Ryu, Key-Zung (Faculty of Biotechnology, College of Applied Life Sciences, Jeju National University) ;
  • Cho, Moon-Jae (Department of Medicine, Medical School, Jeju National University) ;
  • Lee, Dong-Hoon (Citrus Research station, National Institute of horticultural & Herbal Science, Rural Development Administration) ;
  • KimCho, So-Mi (Faculty of Biotechnology, College of Applied Life Sciences, Jeju National University)
  • 김영미 (제주대학교 의과대학 의학과) ;
  • 이도승 (제주대학교 생명자원과학대학 생명공학부) ;
  • 전덕현 (제주대학교 생명자원과학대학 생명공학부) ;
  • 송연우 (제주대학교 생명자원과학대학 생명공학부) ;
  • 이동선 (제주대학교 생명자원과학대학 생명공학부) ;
  • 류기중 (제주대학교 생명자원과학대학 생명공학부) ;
  • 조문제 (제주대학교 의과대학 의학과) ;
  • 이동훈 (국립원예특작과학원 감귤시험장) ;
  • 김소미 (제주대학교 생명자원과학대학 생명공학부)
  • Received : 2010.07.28
  • Accepted : 2011.03.18
  • Published : 2011.04.30
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Abstract

Limonoid UDP-glucosyltransferase (LUGT) is an enzyme that converts limonoids into their corresponding glucosides and ultimately ameliorates limonoid bitterness in Citrus species. In this paper, the LUGT gene was cloned via PCR from 10 Jeju Citrus species. All the deduced glucosyltransferase proteins harbored a highly conserved plant secondary product glucosyltransferase (PSPG) motif within the C terminal region. Phylogenetic analysis based on the amino acid sequence comparison of the LUGT proteins from 10 Citrus species generated three distinct types. The expression patterns of LUGT gene in three representative species from each type were quite different with that of C. unshiu Marc. cv. Miyagawawase(Gungcheon), which his without distinctive juice delayed bitterness. Ourresultssho wth at some Citrus speciessuchas Citrusleiocarpa HORT(Bingul), Citruserythrosa HORT (Dongjunggul), and Citrustachibana TANAKA(Honggul) end emicin Jeju maybe susceptible to intense juice delayed bitterness due to delay inexpression of LUGT.

Limonoid UDP-glucosyltransferase (LUGT)는 리모노이드에 포도당을 붙여줌으로써 궁극적으로 감귤에서 발생하는 limonoid bitterness를 제거해 주는 효소이다. 본 연구에서는 10종의 제주산 감귤로부터 LUGT유전자를 PCR 클로닝하고 그 염기서열을 비교했다. 실험에 사용한 모든 종에서 카르복실기 말단에 식물 당전달 효소에서 발견되는 전형적인 아미노산 서열인 p1ant secondary product glycosyltransferase(PSPG) 모티브가 존재했다. 아미노산 서열에 의한 계통수 분석을 실시해본 결과 10종의 제주산 감귤은 3그룹으로 분류했다. 각 그룹의 대표적인 3종 재래귤 품종 빈귤, 동정귤, 홍귤의 성숙 시기별 LUGT 발현양상은 delayed bitterness가 거의 없는 궁천과는 다른 양상을 나타내었다. 이러한 결과들은 빈귤, 동정귤, 홍귤과 같은 일부 재래귤 품종에서의 쓴맛은 LUGT발현이 지연됨에 따라 발생하는 delayed bitterness에 기인할 수 있음을 시사하고 있다.

Keywords

References

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