Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Antioxidant Activities of Extracts from Fermented Mulberry (Cudrania tricuspidata) Fruit. and Inhibitory Actions on Elastase and Tyrosinase

꾸지뽕열매 발효 추출물의 항산화 특성과 Elastase 및 Tyrosinase 저해활성

  • Kang, Dae-Hun (Sangro Co. Ltd.) ;
  • Kim, Jae-Won (Department of Food Science and Technology, Catholic University of Daegu) ;
  • Youn, Kwang-Sup (Department of Food Science and Technology, Catholic University of Daegu)
  • Received : 2010.10.28
  • Accepted : 2011.03.04
  • Published : 2011.04.30
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Abstract

We evaluated the nutritional value of 70% ethanol extracts (EE) from fermented cudrania tricuspidata fruit (ECT: EE of C. tricuspidata, EFCT: EE of fermented C. tricuspidata, EFCTL: EE of fermented C. tricuspidata by B. licheniformis, EFCTS: EE of fermented C. tricuspidata by B. subtilis) by determined the total polyphenol and flavonoid contents, antioxidant activities, and inhibitory actions on elastase and tyrosinase. The yields of freeze-dried powder of ECT, EFCT, EFCTL, and EFCTS were 54.22%, 54.43%, 57.51%, and 57.23% (each w/w), respectively. The color of $L^*,\;a^*,\;b^*$ values decreased of fermented cudrania tricuspidata. The fermented EFCTL and EFCTS had relatively higher contents of total polyphenol and flavonoid than those of other extracts. ABTS radical scavenging ability were 94.61%, 95.85%, 94.36%, and 96.69%, respectively. SOD (superoxide dismutase)-like activities were in the order EFCT (32.60%) > EFCTS (27.10%) > EFCT (23.30%) > ECT (22.00%), and nitrite scavenging activity was in order of EFCTS (51.18%) > EFCTL (45.61%) > EFCT (41.93%) > ECT (27.76%), respectively. Ferrous ion chelating activity of EFCTL (67.34%) and EFCTS (60.36%) was significantly the highest, whereas ECT (52.34%) and EFCT (51.73%) had not different significantly. Xanthine oxidase, elastase and tyrosinase inhibitory activities at 0.5% (w/v) solutions of EFCTL and EFCTS were somewhat higher than those of non-fermented ECT. In conclusion, we provide experimental evidence that extracts of fermented cudrania tricuspidata of B. subtilis and B. licheniformis exhibited higher antioxidant activities and inhibitory actions on elastase and tyrosinase compared with non fermented cudrania tricuspidata.

발효 꾸지뽕열매의 기능성 소재으로서의 개발 가능성을 알아보고자 70% ethanol 추출물에 대한 항산화 활성과 elastase 및 tyrosinase 저해활성을 측정하였다. 실험군은 꾸지뽕열매 분말 70% ethanol 추출물(ECT), $40^{\circ}C$에서 48시간 발효시킨 분말의 70% ethanol 추출물(FECT), B. lichenifornis 균주로 $40^{\circ}C$에서 48시간 발효시킨 분말의 70% ethanol 추출물(FECTL), B. subtilis 균주로 $40^{\circ}C$에서 48시간 발효시킨 분말의 70% ethanol 추출물(FECTS) 각각을 동결건조하여 실험에 사용하였다. 수율은 ECT, EFCT, EFCTL 및 EFCTS가 각각 54.22%, 54.43%, 57.71% 및 57.23%로 높은 수율을 나타내었으며, 균주 발효 시 증가하는 경향을 보였다. L*, a* 및 b* 값은 발효 처리구에서 감소하였다. Total polyphenol 함량은 ECT 8.13 mg/g (w/w), EFCT 9.53 mg/g (w/w)로 EFCT가 ECT에 비하여 17.22%가 증가하였고, 균주발효군인 EFCTL (11.03 mg/g, w/w) 및 EFCTS (11.90 mg/g, w/w)는 ECT에 비하여 각각 35.67% 및 46.37%가 증가하였다. Total flavonoid 함량 역시 ECT 1.54 mg/g (w/w), EFCT 1.73 mg/g (w/w)로 EFCT가 ECT에 비하여 12.34%가 증가하였으며, EFCTL (1.94 mg/g, w/w) 및 EFCTS (1.85 mg/g, w/w)는 ECT에 비하여 각각 25.97% 및 20.13%가 증가하였다. ABS 라디칼 소거능은 ECT (94.61%), EFCT (95.85%), EFCTL (94.36%), EFCTS (96.69%)로 유사하였다. SOD 유사활성은 ECT (22.00%), EFCT(23.30%), EFCTL (32.60%), EFCTS (27.10%)로 EFCTL에서 높았으며 아질산염소거능에서도 유사한 경향을 나타내었다. Ferrous ion chelating 효과에서는 EFCTL (67.34%) > EFCTS (60.36%) > ECT (52.34%) > EFCT (51.73%) 순이었다. 발효처리군 EFCT, EFCTL 및 EFCTS는 무처리군 ECT에 비하여 xanthine oxidase 저해활성은 각각 9.53%, 38.66% 및 49.78%가, elastase는 19.93%, 86.19% 및 77.60%가, tyrosinase는 23.94%, 89.14% 및 78.86%가 증가하였으며 균주발효군에서 그 활성이 증가하였다. 이상의 결과, 발효 꾸지뽕열매 70% 에탄올 추출물은 항산화활성이 우수하여 기능성 증진용 소재 활용에 효과가 있을 것으로 판단된다.

Keywords

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