Korean Journal of Agricultural Science (농업과학연구)

Institute of Agricultural Science, CNU (충남대학교 농업과학연구소)
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Influence of different strains of Agrobacterium rhizogenes on hairy root induction and growth in Scutellaria baicalensis

Agrobacterium rhizogenes strains이 황금 모상근 유도와 생육에 미치는 영향

  • Park, Woo-Tae (Department of Crop Science, Chungnam National University) ;
  • Kim, Young-Seon (Department of Crop Science, Chungnam National University) ;
  • Park, Nam-Il (Department of Crop Science, Chungnam National University) ;
  • Kim, Haeng-Hoon (National Agrobiodiversity Center, National Academy of Agricultural Science, Rural Development Administration (RDA)) ;
  • Lee, Sook-Young (Chosum University Dental Hospital) ;
  • Park, Sang-Un (Department of Crop Science, Chungnam National University)
  • 박우태 (충남대학교 응용식물학과) ;
  • 김영선 (충남대학교 응용식물학과) ;
  • 박남일 (충남대학교 응용식물학과) ;
  • 김행훈 (농촌진흥청 국립농업과학원 농업유전자원센터) ;
  • 이숙영 (조선대학교 치과병원 의료기기 임상시험센터) ;
  • 박상언 (충남대학교 응용식물학과)
  • Received : 2011.03.17
  • Accepted : 2011.06.20
  • Published : 2011.06.30
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Abstract

Agrobacterium rhizogenes, a gram-negative soil bacterium, is one of the most widely studied among them. A, rhizogenes can transfer T-DNA, excised from Ri (root inducing)-plasmids from the bacterial to the plant cell. It is the causal agent of 'hairy root' diseases in plants, and has been used for the production of hairy root cultures from a multitude of species. Five different strains of Agrobacterium rhizogenes differed in their ability to induce Scutellaria baicalensis hairy roots and also showed varying effects on the growth in hairy root cultures. A. rhizogenes R 1000 is the most effective strain for the induction (57.3%) and growth (11.9 g $L^{-1}$) in hairy root of Scutellaria baicalensis. Our results demonstrate that use of suitable strains of A. rhizogenes may allow study of the regulation of flavone biosynthesis in hairy root cultures of Scutellaria baicalensis.

Keywords

References

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