Korean Journal of Agricultural Science (농업과학연구)

Institute of Agricultural Science, CNU (충남대학교 농업과학연구소)
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Characterization of BLV env gene in Korean Holstein dairy cattle

한국형 홀스타인종 젖소의 BLV env 유전자의 특성분석

  • Jeong, Hang-Jin (Department of Animal Biosystem Science, Chungnam National University) ;
  • Yu, Seong-Lan (Department of Animal Science and Biotechnology, Chungnam National University) ;
  • Lee, Jun-Heon (Department of Animal Science and Biotechnology, Chungnam National University) ;
  • Do, Chang-Hee (Department of Animal Biosystem Science, Chungnam National University) ;
  • Suh, Guk-Hyun (College of Veterinary Medicine, Cheunnam National University) ;
  • Ryoo, Seung-Heui (Government of Changcheongnam-Do, Livestock Research Institute) ;
  • Chung, Sang-Il (College of Veterinary Medicine, Chungnam National University) ;
  • Sang, Byung-Chan (Department of Animal Biosystem Science, Chungnam National University)
  • 정행진 (충남대학교 동물바이오시스템과학과) ;
  • 유성란 (충남대학교 동물자원생명과학과) ;
  • 이준헌 (충남대학교 동물자원생명과학과) ;
  • 도창희 (충남대학교 동물바이오시스템과학과) ;
  • 서국현 (전남대학교 수의과대학) ;
  • 류승희 (충남 축산기술연구소) ;
  • 정상일 (충남대학교 수의과대학) ;
  • 상병찬 (충남대학교 동물바이오시스템과학과)
  • Received : 2011.05.16
  • Accepted : 2011.06.20
  • Published : 2011.06.30
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Abstract

This study was performed to investigate the characterization of infectious BLV env gene isolated form Korean Holstein Cattle and to determine its incoming origin. Gp51 region of BLV env gene known as having important role in immunological function was characterized using PCR-RFLP sequencing and phylogenetic analysis. BLV env gene was grouped into PCR-RFLP patterns with three restriction endonucleases including Pvu II, BamHI and Hae III, and we identified two new RFLP patterns from nucleotide sequences of each group. Phylogenetic analysis showed that 80% of the Korean Holstein was included in the USA and Japanese group. These results here can provide a valuable information about the character of the BLV env gene and research on infection route of BLV.

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References

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