Korean Journal of Agricultural Science (농업과학연구)

Institute of Agricultural Science, CNU (충남대학교 농업과학연구소)
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Sex determination of in vivo- and in vitro-derived bovine embryos

체내 및 체외 수정란의 할구를 이용한 성 판별

  • Han, Rong-Xun (Dept. of Animal Science & Biotechnology, Chungnam National University) ;
  • Kim, Hong-Rye (Dept. of Animal Science & Biotechnology, Chungnam National University) ;
  • Diao, Yun-Fei (Dept. of Animal Science & Biotechnology, Chungnam National University) ;
  • Jin, Dong-Il (Dept. of Animal Science & Biotechnology, Chungnam National University)
  • 한영훈 (충남대학교 동물자원생명과학과) ;
  • 김홍래 (충남대학교 동물자원생명과학과) ;
  • 조운비 (충남대학교 동물자원생명과학과) ;
  • 진동일 (충남대학교 동물자원생명과학과)
  • Received : 2011.04.20
  • Accepted : 2011.06.20
  • Published : 2011.06.30
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Abstract

The objective of this study was to develop a rapid and reliable PCR method for sexing of morula or blastocyst stage bovine embryo. BOV97M and bovine 1.715 satellite DNA sequences were selected for amplification of male and bovine specific DNA, respectively. But the unbalanced number of copies of these two repetitive sequences required some modification of PCR method. Karyotyping of blastomeres were carried for the confirmation of sex determination in bovine embryos. The coincidence rate of sex between biopsied-single blastomere and matched blastocyst was 80.0%. When in vivo- and in vitro- derived embryos were compared, 61.8% and 56.7% were male in in vitro- and in vivo-derived embryos, respectively. In vivo-derived embryos showed better hatching rate than in vitro-derived embryos following biopsy of blastomeres. In conclusion, rapid and effective PCR could be applied to sexing of bovine preimplantation embryos using single blastomere. The sensitivity of this assay may eliminate the need for biopsy of more than one nucleated blastomere and reduce trauma to the embryos derived from biopsy procedure.

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References

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