Korean Journal of Agricultural Science (농업과학연구)

Institute of Agricultural Science, CNU (충남대학교 농업과학연구소)
권호 표지
DOI QR코드

DOI QR Code

Development of clubroot race4 resistant inbreds using conventional breeding and microspore culture method in Chinese cabbage

교배육종 및 소포자 배양에 의한 뿌리혹병 race4 저항성 배추 계통 육성

  • Park, Su-Hyoung (National Institute of Horticultural and Herbal Science, RDA) ;
  • Yoon, Moo-Kyoung (National Institute of Horticultural and Herbal Science, RDA) ;
  • Lim, Yong-Pyo (Department of Horticulture, Chungnam National University)
  • 박수형 (농촌진흥청 국립원예특작과학원) ;
  • 윤무경 (농촌진흥청 국립원예특작과학원) ;
  • 임용표 (충남대학교 원예학과)
  • Received : 2011.12.02
  • Accepted : 2011.12.18
  • Published : 2011.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

To develop clubroot resistant Chinese cabbage inbreds, IT 033820, a clubroot resistant turnip, was cross pollinated with a Chinese cabbage inbred of BP 079. From 2005, conventional breeding and microspore culture method performed using these F1 plants as parental materials. In 2007, conventional breeding method resulted in 21 F3 inbreds. After inoculation of clubroot race 4, one inbred showing 83% resistant was selected and registered as 'onkyo 20036ho' in 2008. From 2005, we scanned hybrid cultivars using micro spore culture and developed many doubled haploid (DH) lines in Chinese cabbage. Using Chinese cultivar of 'oong-baek 2ho' we developed 26 DH inbreds in 2007. After inoculation of clubroot race 4, one DH inbred showing 77% resistant and yellow inner leaf color was selected and registered as 'onkyo 20034'in 2008. We found conventional breeding method was effective using introduced germplasm showing low germination. However, when using hybrid cultivar as starting material, microspore culture method was powerful for developing various inbred in short time.

Keywords

References

  1. Braselton JP. 1995. Current status of the plasmodiophorids. Critical Reviews in Microbiology. 21: 263-275. https://doi.org/10.3109/10408419509113543
  2. Castlebury LA, Domier LL. 1998. Small subunit ribosomal RNA gene phylogeny of Plasmodiophora brassicae. Mycologia. 90: 102-107. https://doi.org/10.2307/3761018
  3. Cheah LH, Veerakone S, Kent G. 2000. Biological control of clubroot on cauliflower with Trichoderma and Streptomyces. Spp. New Zealand Plant Protection 53: 18-21.
  4. Datnoff LE, Kroll TK, Lacy GH. 1987. Efficacy of chlorine for decontaminatining water infected with resting spores of Plasmodiophora brassicae. Plant Disease 71: 734-736. https://doi.org/10.1094/PD-71-0734
  5. Down GJ, Grenville LJ, Clarkson MJ. 2002. Phylogenetic analysis of Spongospora and implications for the taxonomic status of the plasmodiophorids. Mycological Research. 106: 1060-1065. https://doi.org/10.1017/S0953756202006391
  6. Hong SY, Cho KS, Moon JY, Ryu SY, Lee HC, Yoon HK. 2005. Microspore Culture and its Progeny Test for Resistant Line to Clubroot (Plasmodiophora brassicae) in Chinese Cabbage. Kor. J. Hort. Sci. Technol. 23(3): 45-45.
  7. Jang CS, Piao Z, Park YJ, Lim YP. 2001. Development of anther-derived lines resistant to clubroot disease in Chinese cabbage. J. Kor. Soc. Hort. Sci. 42: 689-694.
  8. Kang WJ, Yoon MK, Kim DH, Kim JS, Harn JS, Om YH. 2000. Classification and selection for resistance to clubroot in Cruciferous vegetables. Kor. J. Hort. Sci. Technol. 18(2): 187.
  9. Kim DW, Oh JH. 1997. Incidence, pathogenicity of club root fungus (Plasmodiophora brassicaea) and viral resistance in Chinese cabbage. Korean J. Plant. Pathol. 13: 95-99.
  10. Kim YH, Lee SS. 1995. Culture of microspore of B. campestrisssp. Kor. J. Hort. Sci. Technol. 10: 372-373.
  11. Lee JT, Ru GL, Lee JN, Kweon HJ, Lee EH, Kim WB, Park HY. 2001. Selection of alternative crops on Chinese cabbage in alpine. Kor. J. Hort. Sci. Technol. 19(suppl II): 63.
  12. Lee SS, Nam SC. 1995. Microspore culture of Broccoli. J. Kor. Soc. Hort. Sci. 36(5): 635-640.
  13. Park S, Kwak JH, Yoon MK. 2008. Development of an effective inoculation method for large quantaties of clubroot disease using hydroponics in Chinese cabbage. Kor. J. Hort. Sci. Technol. 26(4): 449-453.
  14. Van de Peer Y, Baldauf SL, Doolittle WF, Meyer A. 2000. An updated and comprehensive rRNA phylogeny of eukaryotes based on rate-calibrated evolutionary distances. J. Mol. Evol. 51: 565-576.
  15. Yeoung YR, Kim JH, Kim BS, Jeon JY, Yoon CS. 2003. Effects of beneficial antagonists (Bacillussp., Pseudomonassp., and Trichodermasp.) on control of club root of Chinese cabbage. 2003. Kor. J. Hort. Sci. Technol. 21(3): 194-198.