Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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Protective Effect of Korean Red Ginseng against Aflatoxin B1-Induced Hepatotoxicity in Rat

  • Kim, Yong-Seong (College of Veterinary Medicine, Chungnam National University) ;
  • Kim, Yong-Hoon (Korea Research Institute of Bioscience and Biotechnology) ;
  • Noh, Jung-Ran (Korea Research Institute of Bioscience and Biotechnology) ;
  • Cho, Eun-Sang (College of Veterinary Medicine, Chungnam National University) ;
  • Park, Jong-Ho (College of Veterinary Medicine, Chungnam National University) ;
  • Son, Hwa-Young (College of Veterinary Medicine and Graduate School of New Drug Discovery and Development, Chungnam National University)
  • Received : 2011.01.19
  • Accepted : 2011.02.22
  • Published : 2011.06.29
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Abstract

Korean red ginseng (KRG), the steamed root of Panax ginseng Meyer, has a variety of biological properties, including anti-inflammatory, antioxidant and anticancer effects. Aflatoxin $B_1$ ($AFB_1$) produced by the Aspergillus spp. causes acute hepatotoxicity by lipid peroxidation and oxidative DNA damage, and induces liver carcinoma in humans and laboratory animals. This study was performed to examine the protective effects of KRG against hepatotoxicity induced by $AFB_1$ using liver-specific serum marker analysis, histopathology, and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling. In addition, to elucidate the possible mechanism of hepatoprotective effects, superoxide dismutase, catalase, glutathione peroxidase, and malondialdehyde were analyzed. Rats were treated with 250 mg/kg of KRG (KRG group) or saline ($AFB_1$ group) for 4 weeks and then received 150 ${\mu}g/kg$ of $AFB_1$ intraperitoneally for 3 days. Rats were sacrificed at 12 h, 24 h, 48 h, 72 h, or 1 wk after $AFB_1$ treatment. In the KRG pre-treatment group, serum alanine aminotransferase, aspartate aminotransferase, and malondialdehyde levels were low, but superoxide dismutase, catalase, and glutathione peroxidase activities were high as compared to the $AFB_1$ alone group. Histopathologically, $AFB_1$ treatment induced necrosis and apoptosis in hepatocytes, and led to inflammatory cells infiltration in the liver. KRG pre-treatment ameliorated these changes. These results indicate that KRG may have protective effects against hepatotoxicity induced by $AFB_1$ that involve the antioxidant properties of KRG.

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References

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