Korean Journal of Poultry Science (한국가금학회지)

The Korean Society of Poultry Science (한국가금학회)
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Molecular Biological Characterization of the First Newcastle Disease Virus Isolated in Mongolia

몽골에서 최초로 분리된 뉴캣슬병 바이러스의 분자생물학적 특성

  • Choi, Kang-Seuk (OIE Reference Laboratory for Newcastle Disease, Avian Disease Division, National Veterinary Research and Quarantine Service) ;
  • Lee, Eun-Kyoung (OIE Reference Laboratory for Newcastle Disease, Avian Disease Division, National Veterinary Research and Quarantine Service) ;
  • Jeon, Woo-Jin (OIE Reference Laboratory for Newcastle Disease, Avian Disease Division, National Veterinary Research and Quarantine Service) ;
  • Batchuulon, D. (State Central Veterinary Laboratory) ;
  • Sodnomdarjaa, R. (State Central Veterinary Laboratory) ;
  • Park, Mi-Ja (OIE Reference Laboratory for Newcastle Disease, Avian Disease Division, National Veterinary Research and Quarantine Service) ;
  • Yoo, Ye-Nah (OIE Reference Laboratory for Newcastle Disease, Avian Disease Division, National Veterinary Research and Quarantine Service) ;
  • Kwon, Jun-Hun (OIE Reference Laboratory for Newcastle Disease, Avian Disease Division, National Veterinary Research and Quarantine Service)
  • 최강석 (국립수의과학검역원 조류질병과 OIE 뉴캣슬병 표준실험실) ;
  • 이은경 (국립수의과학검역원 조류질병과 OIE 뉴캣슬병 표준실험실) ;
  • 전우진 (국립수의과학검역원 조류질병과 OIE 뉴캣슬병 표준실험실) ;
  • ;
  • ;
  • 박미자 (국립수의과학검역원 조류질병과 OIE 뉴캣슬병 표준실험실) ;
  • 유예나 (국립수의과학검역원 조류질병과 OIE 뉴캣슬병 표준실험실) ;
  • 권준헌 (국립수의과학검역원 조류질병과 OIE 뉴캣슬병 표준실험실)
  • Received : 2011.02.14
  • Accepted : 2011.05.09
  • Published : 2011.06.30
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Abstract

The outbreak of Newcastle disease occurred for the first time at a commercial chicken farm near Ulaanbaatar, Mongolia in August 2010. Newcastle disease virus (NDV) obtained from infected chickens in Mongolia was characterized by biological and molecular biological approches. Mongolian NDV isolate killed all of chicken embryos within 60 h in the mean death time assay, indicating virulent for chicken. A genomic region of 695 nts between nts 1055 of the M gene and 508 of the F gene was amplified by RT-PCR and sequenced. The deduced amino acid sequence of the F protein cleavage site was $^{112}RRQKRF^{117}$, which is a typical sequence of velogenic strains of NDV and is agreement with the result of the MDT assay. The sequence of the partial F gene (nts 47 to 435) was used for genotyping by phylogenetic analysis. The phylogenetic analysis showed that the Mongolian isolate was of genotype VII within class II of NDV. Further phylogenetic analysis on the genotype VII strains revealed that the isolates placed in a genetic sublineage of VIId and most closely related with velogenic strains of NDV circulating in Far-east Asian region especially China, suggesting the introduction of velogenic NDV into Mongolia from neighboring countries.

몽골 산란계 농장에서 발생한 뉴캣슬병 사례로부터 뉴캣슬병 바이러스를 분리하고 그 특성을 조사하였다. 그 결과, 발생 농장 산란계 폐사계의 뇌 및 폐 조직으로부터 뉴캣슬병 바이러스 MN1/10 주가 분리되었다. 이 바이러스는 F 단백질 분절 부위가 특징적인 병원성 motif(RRQKRF)를 가지고 있었으며 종란 평균 치사 지수(MDT)가 54.7시간으로 강독형 NDV이었다. 또한 발생 농장 내 생존하고 있는 산란계에서 고역가의 NDV 특이항체가 검출되었다. 유적학적 계통 분석을 실시한 결과, 몽골 분리주는 Class II에 속하는 genotype VIId 바이러스로 확인되었다. 유전학적 계통 분석 결과, 몽골 분리주는 몽골과 인접한 중국에서 유행하는 바이러스 그룹(CN2)에 속하는 것으로 분류되었다. 우리의 연구 결과는 몽골에서의 뉴캣슬병 최초 발생은 동북아시아 지역에서 유행하는 강독형 NDV의 유입에 의해서 이루어졌음을 말해 준다.

Keywords

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