YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Antigenotoxicity of Vegetable or Fruit Extract against Cigarette Smoke Condensate

담배연기응축물의 DNA 손상작용과 야채 및 과일추출물의 보호효과

  • Lee, Hyeong-Ju (College of Pharmacy, Kangwon National University) ;
  • Heo, Chan (College of Pharmacy, Kangwon National University) ;
  • Kim, Nam-Yee (College of Pharmacy, Kangwon National University) ;
  • Heo, Moon-Young (College of Pharmacy, Kangwon National University)
  • 이형주 (강원대학교 약학대학) ;
  • 허찬 (강원대학교 약학대학) ;
  • 김남이 (강원대학교 약학대학) ;
  • 허문영 (강원대학교 약학대학)
  • Received : 2011.05.09
  • Accepted : 2011.06.01
  • Published : 2011.06.30
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Abstract

Cigarette smoke condensate (CSC) is known to be carcinogenic compound. CSC contains many organic compounds such as polycyclic aromatic hydrocarbons (PAHs), and heterocyclic amine compounds (HCAs). Reactive oxygen species (ROS) are also generated and induce oxidative DNA damage during the metabolism of CSC. The rat microsome mediated and DNA repair enzyme treated comet assays together with conventional comet assay were performed to evaluate the mechanisms of CSC genotoxicity. The organic extract of CSC induced oxidative and microsome mediated DNA damage. Vitamin C as a model antioxidant reduced DNA damage in endonuclease III treated comet assay. One of flavonoid, galangin as a CYP1A1 inhibitor, reduced DNA damage in the presence of S-9 mixture. The ethanol extracts of the mixed vegetables (BV) or the mixed fruits (BF) showed potent inhibitory effects against CSC induced DNA damage with oxidative DNA lesions and in the prescence of S-9 mixture. These results indicate that BV and BF could prevent CSC-induced cellular DNA damage by inhibiting oxidative stress and suppressing cytochrome P450 in mammalian cells.

Keywords

References

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