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Inhibitory Effects of Serotonin Derivatives on Adipogenesis

홍화씨 추출물 유래 세로토닌 유도체의 지방전구세포 분화억제 효능에 대한 연구

  • 정은선 (바이오스펙트럼(주) 생명과학연구소) ;
  • 김승범 (바이오스펙트럼(주) 생명과학연구소) ;
  • 김무한 (바이오스펙트럼(주) 생명과학연구소) ;
  • 신승우 (바이오스펙트럼(주) 생명과학연구소) ;
  • 이종성 (바이오스펙트럼(주) 생명과학연구소) ;
  • 박덕훈 (바이오스펙트럼(주) 생명과학연구소)
  • Received : 2011.06.01
  • Accepted : 2011.06.13
  • Published : 2011.06.30

Abstract

N-feruloylserotonin (FS) and N-(pcoumaroyl) serotonin (CS), serotonin derivatives, which have been isolated as major and unique phenolics of safflower seed extract (SSE), are member of hydroxycinnamic acid amides and are implicated in the defense against pathogen infection and insect feeding. In this study, we evaluate inhibitory effects of N-(p-Coumaroyl)serotonin and N-Feruloylserotonin on adipogenesis using oil-red O staining, triglyceride and GPDH activity. we found that while serotonin itself did not suppress differentiation of preadipocytes into adipocytes, N-(p-Coumaroyl)serotonin and N-Feruloylserotonin inhibited the differentiation of preadipocytes into adipocytes in a concentration-dependent manner. In addition, they showed antioxidant effects in DPPH assay. Taken together, these results show that N-feruloylserotonin (FS) and N-(pcoumaroyl) serotonin (CS) suppress differentiation of preadipocytes, suggesting the possibility that these serotonin derivatives can be utilized as an anti-obesity agent.

N-(p-Coumaroyl)serotonin과 N-Feruloylserotonin은 홍화씨에서 유래하는 특이적인 세로토닌유도체로 주로 식물이 병원균을 방어하기 위해서 만들어내는 hydroxycinnamic acid amides계 물질이다. 본 연구에서는 N-(p-Coumaroyl) serotonin과 N-Feruloylserotonin이 지방전구세포의 지방분화에 미치는 영향을 oil-red O염색과 triglyceride 양 측정, GPDH 활성 측정 등을 통해 알아보았고, 그 결과 두 물질 모두 유의적으로 지방세포분화를 억제함을 관찰하였다. 효능 비교물질로 세로토닌을 처리했을 때 세로토닌 자체로는 지방분화에 유의미한 효과는 관찰되지 않았다. N-(p-Coumaroyl) serotonin과 N-Feruloylserotonin은 또한 우수한 항산화능을 보여주었다. 이러한 결과를 통해, N-(p-Coumaroyl)serotonin과 N-Feruloylserotonin이 지방세포분화억제를 통한 항비만 소재로서의 가능성을 확인하였다.

Keywords

References

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