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부산, 울산 및 경상남도 지역의 지하수 중 norovirus 오염실태 조사

Groundwater Contamination of Noroviruses in Busan, Ulsan, and Gyeongsangnam-do, Korea

  • Park, Byung-Ju (Department of Microbiology, College of Natural Sciences, Pusan National University) ;
  • Oh, Hae-Ri (Department of Microbiology, College of Natural Sciences, Pusan National University) ;
  • Kang, Ho-Young (Department of Microbiology, College of Natural Sciences, Pusan National University) ;
  • Jang, Kyung-Lib (Department of Microbiology, College of Natural Sciences, Pusan National University)
  • 투고 : 2011.03.18
  • 심사 : 2011.05.20
  • 발행 : 2011.06.30

초록

본 연구에서는 2009년부터 2010년까지 부산, 경남 및 울산 지역의 145 개 지하수 시료들을 대상으로 norovirus의 오염실태를 조사하였다. 먼저 norovirus의 검출을 위한 두 세트의 primer를 제작하였으며 이를 이용하여 최적 nested reverse transcription (RT)-PCR 조건을 확립하였다. 지하수의 norovirus 오염실태 조사에 의하면, 건기(4월~6월)와 우기(7월~8월)에 각각 21개(25.9%)와 15개(23.4%)의 시료에서 norovirus가 검출되었다. 부산, 울산 및 경상남도의 시료에서 각각 15%, 7% 및 32%의 norovirus가 검출되어 지역적인 차이를 나타내었다. norovirus 양성시료에서 GI과 GII가 각각 5개와 31개가 검출되어GI에 비하여 GII가 우세함을 알 수 있었다. norovirus 분리주들의 계통분류학적 분석 결과에 의하면, GI 분리주들은 모두 GI.5 유전자형으로 분류되었으며, GII 분리주들은 GII.3과 GII.4로 양분되었다. norovirus의 검출은 pH, 온도, 산화-환원전위 및 용존산소 등의 이화학적 인자들과 일반세균, 총대장균군 및 대장균과 같은 미생물 수질지표들과 통계적인 유의성이 있는 상관관계를 나타내지 않았다. 반면에 norovirus의 검출과 저탁도(<0.50 NTU) 사이에는 밀접한 상관성이 있음이 밝혀졌다. 본 연구를 통하여 지하수 중 norovirus의 분포실태를 어느 정도 파악할 수 있었으며 수인성 질병의 예방과 국민 보건위생을 위해서는 지하수의 주기적인 norovirus 모니터링이 필요함을 제시하였다.

To inspect norovirus contamination of groundwater in south eastern areas of Korea, a systematic survey of groundwater in Busan, Ulsan, and Gyeongsangnam-do was performed for two years from 2009 to 2010. For this purpose, we first optimized the nested reverse transcription-PCR condition by designing two sets of primers for the detection of norovirus genogroups, GI and GII. Of 145 samples, 21 (25.9%) and 15 (23.4%) were norovirus positive in the dry season (April to June) and wet season (July to August), respectively. The detection frequencies of norovirus in Busan, Ulsan, and Gyeongsangnam-do were 15%, 7%, and 32%, respectively, reflecting a geographical difference in their distribution. The GI and GII isolates were 5 and 31, respectively, indicating the prevalence of GII in the tested areas. According to phylogenetic analysis of their nucleotide sequences, all of the GI isolates were identified to genotype GI.5 whilst the GII isolates were divided into two genotypes, GII.3 and GII.4. Neither physical-chemical parameters such as pH, temperature, oxidation-reduction potential, and dissolved oxygen, nor microbial indicators of water quality such as total bacteria, total coliforms, and Escherichia coli were statistically correlated with contamination of norovirus in the groundwater. Interestingly, however, the presence of norovirus was closely correlated with low turbidity (<0.50 NTU). The present study suggests that periodical monitoring of norovirus in groundwater is necessary to prevent epidemic waterborne diseases and to secure better sanitary conditions for public health.

키워드

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