DOI QR코드

DOI QR Code

수영부하 피로에 대한 마늘추출물 및 비타민 B군 강화 급이의 회복 효과

Recovery Effect of Garlic Extract and Vitamin B group Enhanced Diet on Swimming Fatigue

  • 투고 : 2011.04.28
  • 심사 : 2011.05.02
  • 발행 : 2011.06.30

초록

마늘추출물 및 비타민 B군 강화 식이를 4주간 급이한 후 흰쥐에 수영부하 실험으로 스트레스를 가하였을 때, 피로회복 인자와 관련된 흰쥐의 체내 지질성분 및 간 기능에 미치는 영향을 분석하였다. 혈당은 대조군($193.41{\pm}3.85$ mg/dl)보다 실험식이군이 $152.85{\pm}6.57{\sim}169.93{\pm}8.75$ mg/dl로 유의적으로 낮았다. 혈청 중 총 지질, 총 콜레스테롤 및 LDL-콜레스테롤은 대조군에 비해 낮은 경향이었으며 마늘추출물과 비타민 B군 강화 시료를 각각 첨가한 SG군과 SV군 보다는 이들을 혼합 급이한 SGV군에서 유의적으로 더 낮았다. 혈청 중의 TBARS 함량은 정상군에 비해 대조군에서 17.25%나 증가하였으며 마늘추출물 및 비타민 B군 강화 급이로 낮아지는 경향이었다. 간조직의 지질 성분 중 총지질은 정상군에 비해 SGV군이 10% 더 낮은 함량이었고, 중성지질은 대조군에 비해 SGV군에서 46% 더 낮게 정량 되었다. 수영부하 스트레스에 의한 간조직 중의 글리코겐 함량은 대조군이 정상군보다 유의적으로 낮았으며 SG, SV 및 SGV 군은 정상군과 유의차가 없었다. 이상의 결과를 종합하여 볼 때 마늘추출물과 비타민 B군 강화급이는 혈중 혈당, 총콜레스테롤 및 LDL-콜레스테롤을 유의적으로 감소시켰으며 또한 수영부하 스트레스로 혈중 및 간장에 다량 생성될 수 있는 지질과산화물 함량도 유의적으로 감소시키므로 피로회복 및 스트레스 개선에 효과가 있을 것으로 판단된다.

The purpose of this study was to investigate the effects of a diet supplemented with garlic extract and vitamin B complex on fatigue recovery from swimming load test in rats. Sprague-Dawley rats were divided into a non-swimming control group (Normal) and swimming load test groups. Swimming load test groups were re-divided into swimming control group (Control), 1% of 60 brix garlic extract fed group (SG), vitamin B complex twice mixture fed group (SV) and 1% of 60 brix garlic extract and vitamin B group twice mixture fed group (SGV). Concentration of plasma lipids and glucose were determined in the rats post-swimming. Concentration of plasma glucose, total lipid, total cholesterol and triglyceride were significantly increased in the control group compared to the non-swimming control group (p<0.05), and these levels were shown to be significantly lower in the SG, SV and SGV groups than in the control group. The SGV group showed the lowest level, however, there was no significant difference between the SG and SV groups. There was no significant difference in HDL-cholesterol concentrations between the normal group and control group, but its concentration was the highest in the SG ($45.95{\pm}5.52$ mg/dl) group. LDL-cholesterol concentration was the highest in the control group, but it was decreased significantly in the SGV group. In conclusion, this study demonstrates that a diet supplemented with 1% 60 brix garlic extract and vitamin B group twice mixture has beneficial effects on fatigue recovery in swimming load tests.

키워드

참고문헌

  1. Ahlborg, G. P., L. Felig, R. H. Hender, and J. Wahren. 1974. Substrate turnover during prolonged exercise in man: Splanchnic and leg metabolism of glucose, FFA and amino acid. J. Clin. Invest. 53, 1080-1090. https://doi.org/10.1172/JCI107645
  2. Altland, P. D. and B. Highman. 1961. Effect of exercise on serum enzyme values and tissues of rat. American J. Physiol. 201, 393-395.
  3. Ariga, T., S. Oshiha, and T. Tamada. 1981. Platelet aggregation inhibitor in garlic. Lancet 1, 150-212.
  4. Azevedo, J., L. Jr, J. K. Linderman, S. L. Lehman, and G. A. Brooks. 1998. Training decreases muscle glycogen turnover during exercise. Eur. J. Appl. Physiol. Occup. Physiol. 78, 479-486. https://doi.org/10.1007/s004210050449
  5. Baek, Y. H. 1995. Effect of garlic intake on the antifatigue and fatigue recovery during prolonged exercise. J. Korean Soc. Food Nutr. 24, 970-977.
  6. Belman, S. 1983. Onion and garlic oils inhibit tumor promotion. Carcinogenesis 4, 1063-1067. https://doi.org/10.1093/carcin/4.8.1063
  7. Chi, M. S., E. T. Kor, and T. J. Stewart. 1982. Effect of garlic on lipid metabolism in rats fed cholesterol or lard. J. Nutr. 122, 241-248.
  8. Choi, H. M., S. C. Lee, S. P. Ryu, I. K. Rhee, G. J. Joo, and S. J. Rhee. 2001. Effects of gluconic acid derivatives isolated from xylan on anti oxidative defense system and muscle fatigue recovery after aerobic exercise. Korean J. Nutr. 34, 872-880.
  9. Davies, K. J. A., A. T. Quintaniham, G. A. Brooks, and L. Packer. 1982. Free radical and tissue damage produced by exercise. Biochem. Biophys. Res. Commun. 107, 1198-1205. https://doi.org/10.1016/S0006-291X(82)80124-1
  10. Folch, J., M. Lees, and G. H. Stanley. 1957. A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 226, 497-502.
  11. Friedewald, W. T., R. I. Levy, and D. S. Fredrickson. 1972. Estimation of the concentration of low density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin. Chem. 18, 499-502.
  12. Hassan, I., E. I. Sayad, M. Amoura, A. E. Naga, A. A. Gadallah, and I. H. Bakr. 2010. Protective effect of Allium against defects of hypercholesterolemia on pregnant rats and their offspring. Int. J. Clin. Exp. Med. 3, 152-163.
  13. Ichikawa, M, J. Yoshida, N. Ide, T. Sasaoka, H. Yamaguchi, and K. Ono. 2006. Tetrahydro-$\beta$-carboline derivatives in aged garlic extract show antioxidant properties. J. Nutr. 136, 726-731.
  14. Hikino, H., M. Tohkin, Y. Bukiso, T. Namiki, S. Nishiumurs, and K. Takeyama. 1986. Antihepatotoxin action of Allium Sativum bulbs. Planta Medica 52,163-168. https://doi.org/10.1055/s-2007-969111
  15. Imai, J., N. Ide, S. Nagae, and T. Moriguchi. 1994. Antioxidant and radical scavenging effects of aged garlic extract and its constituents. Planta Medica 60, 417-420. https://doi.org/10.1055/s-2006-959522
  16. Jone, B. D., J. H. Kim, and S. Ryu. 2009. Differences of garlic power ingestion and exercise training on blood lipids, MDA and SOD in rats. J. Life Sci. 19, 1337-1345. https://doi.org/10.5352/JLS.2009.19.10.1337
  17. Jung, H. S., E. Y. Kim, E. S. Shin, H. S. Lee, E. J. Moon, Z. H. Jin, S. Y. Kim, Y. J. Sohn, and N. W. Shon. 2008. Effects of Rhodiolarosea (KH101) on Anti-fatigue forced swimming rats. Korean J. Orient Int. Med. 29, 922-938.
  18. Karlsson, J. 1971. Lactate and phosphagen concentrations in working muscle of man. Acta. Physiol. Scand Supple. 1, 358.
  19. Koo, H. N., J. K. Lee, S. H. Hong, and H. M. Kim. 2004. Herbkines increase physical stamina in mice. Biol. Pharm. Bull. 27, 117-119. https://doi.org/10.1248/bpb.27.117
  20. Lee, H. Y., I. Y. Park, and J. H. Woo. 2005. The effects of pyruvate supplementation on blood fatigue factors and lipid components during prolonged exercise. Korean J. Sport Sci. 16, 18-26.
  21. Lee, S. J., M. J. Kang, J. H. Shin, J. H. Kim, S. K. Kang, and N. J. Sung. 2009. The effect garlic and medicinal plant extracts on the liver function and lipid metabolism of rats administered with alcohol. J. Korean Soc. Food Sci. Nutr. 38, 561-568. https://doi.org/10.3746/jkfn.2009.38.5.561
  22. Lee, S. J., H. Nam, M. M. Kim, H. J. Jang, A. J. Park, B. W. Kim, and K. T. Chung. 2010. In vitro inhibitory effect of aged black garlic extract with antioxidant activity on MMP-2 and MMP-9 related to metastasis. J. Life Sci. 20, 760-767. https://doi.org/10.5352/JLS.2010.20.5.760
  23. Lo, S., J. C. Russel, and W. Taylor. 1970. Determination of glycogen in small tissue. Appl. Physiol. 28, 234-236.
  24. Moch, S. B., M. H. Woo, H. W. Lee, J. W. Choi, and H. S. Kim 2009. Effects of herbal sports drinks with Omija, Maesil and Molasses on the endurance and energy metabolites of experimental animals. J. Life Sci. 19, 219-227. https://doi.org/10.5352/JLS.2009.19.2.219
  25. Morihara, N., I. Sumioka, T. Moriguchi, N. Uda, and E. Kyo. 2002. Aged garlic extract enhances production of nitric oxide. Life Sci. 71, 509-517. https://doi.org/10.1016/S0024-3205(02)01706-X
  26. Park, D. I. and I. J. Cho. 1997. The effect of Hwangkikunjungtang and Gamihwangkikunjoongtang on the recovery of exercise induced fatigue. J. Korean Orient. Med. Soc. 18, 155-166.
  27. Park, S. H., J. S. Kwak, S. J. Park, and J. H. Han. 2004. Effect of beverage including extracts of Artemisia capillaries on fatigue recovery materials, heart rate and serum lipids in university male athletes. J. Korean Soc. Food Sci. Nutr. 33, 839-846. https://doi.org/10.3746/jkfn.2004.33.5.839
  28. Park, Y. Y. and D. I. Park. 1998. The effects of samultang and samultang-mixed-kamibulhwan-gumjungkisan on the recovery of exercise induced fatigue. Korean J. Life Sci. 8, 241-248.
  29. Seo, H. J. 1999. Effect of garlic on the blood lipids and other serum components in rats. J. Korean Soc. Food Sci. Nutr. 28, 1339-1348.
  30. Sharma, K. K., A. L. Sharma, K. K. Dwivedi, and P. K. Sharma. 1976. Effect of raw and boiled garlic on blood cholesterol in butter fat lipasemia. Ind. J. Nutr. Dietet 13, 7-10.
  31. Uchiyama, M. and M. Mihara. 1978. Determination of malondialdehyde precursor in tissues by TBA test. Anal. Biochem. 86, 271-278. https://doi.org/10.1016/0003-2697(78)90342-1
  32. Woo, K. S. 2005. Effects of high temperature and pressure treatment on the physicochemical characterics of licorice (Glycyrrhizauralemsis Fish). Chungbuk University, Cheongju, Korea.
  33. Yagi, K. 1984. Assay for blood plasma or serum. In: Method in enzymology. pp. 328-331. Academic Press, New York
  34. Yu, Y. Y. and Y. M. Shaw. 1994. Garlic reduces plasma lipids by inhibiting hepatic cholesterol and triacylglycerol synthesis. Lipids 29, 189-193. https://doi.org/10.1007/BF02536728
  35. Yoon, G. A. 2006. Effect of garlic supplement and exercise on plasma lipid and antioxidant enzyme system in rats. Korean Nutr. Soc. 39, 3-10.
  36. Yoon, G. A. 2007. Antioxidant effect of garlic supplement against exercise-induced oxidation stress rats. Korean Nutr. Soc. 40, 701-707.
  37. Yoon, Y. H. 1993. The effect of exercise intensity on serum LDH activity and isoenzyme pattern. Dong-A University, Busan, Korea

피인용 문헌

  1. Quality and Fermentation Characteristics of Garlic-added Kimchi vol.19, pp.4, 2012, https://doi.org/10.11002/kjfp.2012.19.4.539
  2. Simultaneous Determination of Water Soluble Vitamins B Group in Health Functional Foods etc. by HPLC vol.30, pp.2, 2015, https://doi.org/10.13103/JFHS.2015.30.2.143
  3. Effect of Black Garlic and Herb Formulas on Lipid Profiles and Antioxidant Status in Rats by Interval Running Training vol.23, pp.12, 2013, https://doi.org/10.5352/JLS.2013.23.12.1436
  4. Antioxidant and anti-fatigue effects of abalone (Haliotis discus hannai) composites containing natural plant vol.22, pp.4, 2015, https://doi.org/10.11002/kjfp.2015.22.4.598