The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Anti-oxidation and Anti-aging Effect of Mixed Extract from Korean Medicinal Herbs

한약재 복합 추출물이 항산화 및 항노화에 미치는 영향

  • Hwang, Jae-Gyu (Daegu Technopark Oriental Medicine Industry Support Center) ;
  • Yun, Jong-Kuk (Daegu Technopark Oriental Medicine Industry Support Center) ;
  • Han, Kil-Hwan (Daegu Technopark Oriental Medicine Industry Support Center) ;
  • Do, Eun-Ju (Daegu Technopark Oriental Medicine Industry Support Center) ;
  • Lee, Jin-Sang (Daegu Technopark Oriental Medicine Industry Support Center) ;
  • Lee, Eun-Ju (NUC Electronics Co., Ltd.) ;
  • Kim, Jong-Boo (NUC Electronics Co., Ltd.) ;
  • Kim, Mi-Ryeo (Daegu Technopark Oriental Medicine Industry Support Center)
  • 황재규 ((재)대구테크노파크 한방산업지원센터) ;
  • 윤종국 ((재)대구테크노파크 한방산업지원센터) ;
  • 한길환 ((재)대구테크노파크 한방산업지원센터) ;
  • 도은주 ((재)대구테크노파크 한방산업지원센터) ;
  • 이진상 ((재)대구테크노파크 한방산업지원센터) ;
  • 이은주 ((주)엔유씨전자) ;
  • 김종부 ((주)엔유씨전자) ;
  • 김미려 ((재)대구테크노파크 한방산업지원센터)
  • Received : 2011.02.16
  • Accepted : 2011.03.10
  • Published : 2011.03.30
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Abstract

Objectives : The present study was designed to investigate effects of mixed extract from korean medicinal herbs (MIX) on oxidation/reduction reaction-related and aging-related enzyme in vitro. Methods : We performed MTT assay, collagenase inhibition assay, elastase inhibition assay, tyrosinase inhibition assay, DPPH free radical scavenging assay, SOD-like activity and xanthine oxidase inhibition assay. Results : Recently, many studies have reported that elastin is also involved in inhibiting or repairing wrinkle formation, although collagen is a major factor in the skin wrinkle formation. The MIX showed 97% inhibition of collagenase activity, and 64% inhibition of elastase activity at 1 mg/ml concentration of MIX, next only to positive control, which indicate good efficacy for anti-wrinkle ingredient. Also it's treatment showed 34% inhibition of tyrosinase activity, to relate whitening effect, at the same dose of MIX. Antioxidant activities were determined by DPPH radical scavenging, xanthine oxidase (XO) inhibiting activity and SOD-like activity. Also these scavenging, XO-inhibiting and SOD-like activities were measured in 91%, 80%, and 63% inhibition, respectively, at a treated dose of 1 mg/ml, compare to control. Conclusions : These results suggest that possibility of mixed korean medicinal herbs as a functional ingredient for anti-wrinkle and whitening, anti-oxidation and anti-aging cosmetic formula.

Keywords

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