Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Protective Effect of Enerbalance on Cadmium-induced Testicular Damages in Mice

카드뮴에 의해 유도된 마우스의 고환 독성 모델에서 에너발란스의 보호 효과

  • Park, Kwang-Hyun (Department of Biochemistry, Chonbuk National University Medical School) ;
  • Mok, Ji-Ye (School of Alternative Medicine & Health Science, College of Alternative Medicine, Jeonju University) ;
  • Kim, Sung-Zoo (Department of Physiology and Center for Healthcare Technology Development, Chonbuk National University Medical School) ;
  • Kang, Hyung-Sub (Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Chonbuk National University) ;
  • Shim, Jae-Suk (Imsil Herbal Medicine Association) ;
  • Jang, Seon-Il (School of Alternative Medicine & Health Science, College of Alternative Medicine, Jeonju University)
  • 박광현 (전북대학교 의과대학 생화학교실) ;
  • 목지예 (전주대학교 대체의학대학 대체건강관리학부) ;
  • 김성주 (전북대학교 의과대학 생리학교실과 헬스케어사업단) ;
  • 강형섭 (전북대학교 수의과대학 약리학교실) ;
  • 심재석 (임실생약영농조합법인) ;
  • 장선일 (전주대학교 대체의학대학 대체건강관리학부)
  • Received : 2011.03.09
  • Accepted : 2011.06.10
  • Published : 2011.06.25
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Abstract

Cadmium (Cd) is well known as a spermatotoxic and gonadotoxic heavy metal ion. This study was performed to assess the possible protective effect of Enerbalance on Cd-induced spermiotoxicity and testicular damage. The control group received isotonic saline; Cd group received Cd (2 mg/kg BW per day) orally; extract-treated groups were orally administrated with Enerbalance (50 mg and 100 mg/kg BW per day) and Cd for 10 days. Morphological changes of testicular tissue, sperm characteristics, oxidative/antioxidative parameters from testis, and serum sexual hormone level were determined. Enerbalance was significantely increased sperm amount in cauda epididymis without changes of ratio of epididymis/body weight and testis/body weight. Cd caused a marked decrease in epididymal sperm concentration and chemotactic sperm motility, testicular superoxide dismutase (SOD), catalase (CAT), Enerbalance was significantly ameliorated loss of epididymal sperm concentration, sperm chemotactic motility, antioxidative parameters, and male hormone whereas decreased abnormal architecture by testis damage. Enerbalance was successfully attenuated these adverse effects of Cd and offers a dose-dependent protection. Our study demonstrated that Enerbalance could proffer a measure of protection against Cd-induced testicular damage and spermiotoxicity by possibly reducing oxidative stress and increasing the antioxidant defense mechanism in mice.

Keywords

References

  1. World Health Organization, Environmental Health Criteria 134, Cadmium. International Programme on Chemical Safety (IPCS), Geneva, 1992.
  2. Satarug, S., Baker, J.R., Urbenjapol, S., Haswell-Elkins, M., Reilly, P.E., Williams, D.J., Moore, M.R. A global perspective on cadmium pollution and toxicity in non-occupationally exposed population. Toxicol. Lett. 137: 65-83, 2003. https://doi.org/10.1016/S0378-4274(02)00381-8
  3. Hew, K.W., Ericson, W.A., Welsh, M.J. A single low cadmium dose cause failure of spermiation in the rat. Toxicol. Appl. Pharmacol. 121: 15-21, 1993. https://doi.org/10.1006/taap.1993.1123
  4. Xu, G., Jiang, X.Z. Male reproductive toxicity of cadmium. J. Chin. Public. Health. 15: 17-18, 1996.
  5. Laskey, J.W., Phelps, P.V. Effects of cadmium and other metal cations on other metal cations on in vitro Leydig cell testosterone production. Toxicol. Appl. Pharmacol. 108:296-306, 1991. https://doi.org/10.1016/0041-008X(91)90119-Y
  6. Oteiza, P.I., Adonaylo, V.N., Keen, C.L. Cadmium- induced testes oxidative damage in rats can be influenced by dietary zinc intake. Toxicology. 137: 13-22, 1999. https://doi.org/10.1016/S0300-483X(99)00067-0
  7. El-Demerdash, F.M., Yousef, M.I., Kedwany, F.S., Bahadadi, H.H. Cadmiuminduced changes in lipid peroxidation, blood hematology, biochemical parameters and semen quality of male rats: protective role of vitamin E and $\beta$-carotene. Food. Chem. Toxicol. 42: 1563-1571, 2004. https://doi.org/10.1016/j.fct.2004.05.001
  8. Yang, H.S., Han, D.K., Kim, J.R., Sin, J.C. Effect of $\alpha$-tocopherol on cadmium induced toxicity in rat testis and spermatogenesis. J. Korean Med. Sci. 21: 445-451, 2006. https://doi.org/10.3346/jkms.2006.21.3.445
  9. Suzuki, T., Yoshida, A. Long-term effectiveness of dietary iron and ascorbic acid in the prevention and cure of cadmium toxicity in rats. Am. J. Clin. Nutr. 31: 1491-1498, 1978. https://doi.org/10.1093/ajcn/31.9.1491
  10. Sen Gupta, R., Sen Gupta, E., Dhakal, B.K., Thakur, A.R., Ahnn, J. Vitamin C and vitamin E protect the rat testes from cadmium-induced reactive oxygen species. Mol. Cells. 17: 132-139, 2004.
  11. Manna, P., Sinha, M., Sil, P.C. Cadmium induced testicular pathophysiology: prophylactic role of taurine. Reprod. Toxicol. 26: 282-291, 2008. https://doi.org/10.1016/j.reprotox.2008.09.009
  12. Cao, G., Sofic, E., Prior, R.L. Antioxidant capacity of tea and common vegetables. J. Agric. Food. Chem. 44: 3426-3441, 1996. https://doi.org/10.1021/jf9602535
  13. Velioglu, Y.S., Mazza, G., Gao, L., Oomah, B.D. Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. J. Agric. Food. Chem. 46: 4113-4117, 1998. https://doi.org/10.1021/jf9801973
  14. Ames, B.M., Shigena, M.K., Hagen, T.M. Oxidants antioxidants and the degenerative diseases of aging. Proc. Natl. Acad. Sci. U.S.A. 90: 7915-7922, 1993. https://doi.org/10.1073/pnas.90.17.7915
  15. Dragsted, L.O., Strube, M., Larsen, J.C. Cancer-protective factors in fruits and vegetables: biochemical and biological background. Pharmacol. Toxicol. 72(Suppl. 1):116-135, 1993.
  16. Verlangieri, A. J., Kapeghian, J. C., El-Dean, S., Bush, M. Fruit and vegetable consumption and cardiovascular mortality. Med. Hypoth. 16: 7-15, 1985. https://doi.org/10.1016/0306-9877(85)90035-0
  17. Ascherio, A., Rimm, E.B., Giovannucci, E.L., Colditz, G.A., Rosner, B., Willett, W.C., Sacks, F., Stampfer, M.J. A prospective study of nutritional factors and hypertension among US men. Circulation. 86: 1475-1484, 1992. https://doi.org/10.1161/01.CIR.86.5.1475
  18. Sacks, F.M., Kass, E.H. Low blood pressure in vegetarians: effects of specific food and nutrients. Am. J. Clin. Nutr. 48: 795-800, 1988. https://doi.org/10.1093/ajcn/48.3.795
  19. Kim, Y.C., Kim, M.S., Lee, N.K., Lee, J.H. Effects of water extracts of Torilis Fructus, Rubus Fructus, Schizandrae Fructus, Lycil Fructus and Cuscutae Semen on the immune system in mice fed with protein deficient diet. The Proceedings of 15th Annual Convention. 16(1):48-49, 1985.
  20. Kim, I.D., Kwon, R.H., Hur, Y.H., Jung, H.J., Kang, H.Y., Ha, B.J. Supercritical extraction of oriental herb: Anti-aging and anti-wrinkle effects. Korean. J. Biotechnol. Bioeng. 23: 529-534, 2008.
  21. Linder, R.E., Strader, L.F., McElroy, W. K. Measurement of epididymal sperm motility as a test variable in the rat. Bull. Environ. Contam. Toxicol. 36: 317-324, 1986. https://doi.org/10.1007/BF01623514
  22. Ralt, D., Manor, M., Cohen-Dayag, A., Tur-Kaspa, I., Ben-Shlomo, I., Makler, A., Yuli, I., Dor, J., Blumberg, S., Mashiach, S., Eisenbach, M. Chemotaxis and chemokinesis of human spermatozoa to follicular factors. Biol. Reprod. 50: 774-785, 1994. https://doi.org/10.1095/biolreprod50.4.774
  23. Bradford, M.M. Rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254, 1976. https://doi.org/10.1016/0003-2697(76)90527-3
  24. Wang, S.Y., Lin, H.S. Antioxidant activity in fruits and leaves of blackberry, raspberry, and strawberry varies with cultivar and developmental stage. J. Agric. Food. Chem. 48: 140-146, 2000. https://doi.org/10.1021/jf9908345
  25. Kim, J.O., Kim, K.S., Lee, G.D., Kwon, J.H. Antihyperglycemic and antioxidative effects of new herbal formula in streptozotocin-induced diabetic rats. J. Med. Food. 12: 728-735, 2009. https://doi.org/10.1089/jmf.2008.1195
  26. Liu, J.H., Ho, S.C., Lai, T.H., Liu, T.H., Chi, P.Y., Wu, R.Y. Protective effects of Chinese herbs on D-galactose-induced oxidative damage. Methods. Find. Exp. Clin. Pharmacol. 25: 447-452, 2003. https://doi.org/10.1358/mf.2003.25.6.769650
  27. Li, Y., Ye, M., Liu, H., Ji, X., Yan, Y. Analysis of flavonoids in Semen Cuscutae by micellar electrokinetic chromatography and high-performance liquid chromatoraphy. J. Capill. Electrophor. Microchip. Technol. 6: 157-162. 1999.
  28. Sharma, R.K., Argawal, A. Role of reactive oxygen species in male infertility. Urology. 48: 835-850, 1996. https://doi.org/10.1016/S0090-4295(96)00313-5
  29. Agarwal, A., Saleh, R.A. Role of oxidants in male infertility: rationale, significance, and treatment. Urol. Clin. North. Am. 29: 817-827, 2002. https://doi.org/10.1016/S0094-0143(02)00081-2
  30. Vernet, P., Aitken, R.J., Drevet, J.R. Antioxidant strategies in the epididymis. Mol. Cell. Endocrinol. 216: 31-39, 2004. https://doi.org/10.1016/j.mce.2003.10.069
  31. Shen, Y., Sangiah, S. Na+, K+-ATPase, glutathione, and hydroxyl free radicals in cadmium chloride-induced testicular toxicity in mice. Arch. Environ. Contam. Toxicol. 29: 174-179, 1995.
  32. Wasowicz, W., Gromadzinska, J., Rydzynski, K. Blood concentration of essential trace elements and heavy metals in workers exposed to lead and cadmium. Int. J. Occup. Med. Environ. Health. 14: 223-229, 2001.
  33. Cox, R.M., John-Alder, H.B. Testosterone has opposite effects on male growth in lizards (Sceloporus spp.) with opposite patterns of sexual size dimorphism. J. Exp. Biol. 208(Pt 24):4679-4687, 2005. https://doi.org/10.1242/jeb.01948
  34. Reed, W.L., Clark., M.E., Parker, P.G., Raouf, S.A., Arguedas, N., Monk, D.S., Snajdr, E., Nolan, V., Ketterson, E.D. Physiological effects on demography: a long-term experimental study of testosterone's effects on fitness. Am. Nat. 167: 667-683, 2006. https://doi.org/10.1086/503054
  35. Mooradian, A.D., Morley, J.E., Korenman, S.G. Biological actions of androgens. Endocr. Rev. 8: 1-28, 1987. https://doi.org/10.1210/edrv-8-1-1