Experimental Study on the Expression of Hair Growth Related Factors after Bee Venom Acupuncture Therapy

봉독약침요법(蜂毒藥鍼療法)에 의한 발모관련 인자들의 발현에 대한 연구

  • Kim, Ho-Il (Department of Anatomy, College of Oriental Medicine, Woosuk University) ;
  • Kim, Cheong-Moo (Department of Anatomy, College of Oriental Medicine, Woosuk University) ;
  • Shin, Hyun-Jong (Department of Oriental Pathology, College of Oriental Medicine, Woosuk University) ;
  • Lee, Chang-Hyun (Department of Anatomy, College of Oriental Medicine, Woosuk University)
  • 김호일 (우석대학교 한의과대학 해부학교실) ;
  • 김정무 (우석대학교 한의과대학 해부학교실) ;
  • 신현종 (우석대학교 한의과대학 한방병리학교실) ;
  • 이창현 (우석대학교 한의과대학 해부학교실)
  • Received : 2011.04.04
  • Accepted : 2011.06.02
  • Published : 2011.06.25

Abstract

The present study was undertaken to investigate the effect of bee venom acupuncture therapy on the hair follicle growth by macroscopic, microscopic and immunohistochemical observation of skin 10 and 17 days after treatment. The results were as follows : Macroscopic hair follicle growth of 0.2 ml S.B.V. acupuncture treated group was more prominent than those of 0.1 ml S.B.V. acupuncture treated group and control group. Microscopic observation indicated that the hair follicle growth of control group reached anagen phase IV of hair growing cycle, and that of 0.1 ml and 0.2 ml S.B.V. acupuncture treated groups reached anagen phase VI and catagen, respectively. Immunohistochemical observations of the expression of various cytokines, enzymes and receptors in association with hair follicle cycle after local treatment of S.B.V. acupuncture therapy are as follows: Expression of fibroblast growth factor was more intense in epidermis and outer root sheath in 0.2 ml S.B.V. acupuncture treated group than that of 0.1 ml S.B.V. acupuncture treated group and control group. Expression of epidermal growth factor was more intense in bulge and outer root sheath in 0.2 ml S.B.V. acupuncture treated group than that of 0.1 ml S.B.V. acupuncture treated group and control group. Expression of c-kit receptor was more intense in epidermis, bulge and outer root sheath in 0.2 ml S.B.V. acupuncture treated group than that of control group. Expression of vascular endothelial growth factor was more intense in epidermis, bulge and outer root sheath in 0.2 ml S.B.V. acupuncture treated group than that of control group. Expression of protein kinase C-${\alpha}$ was more intense in epidermis, bulge and outer root sheath in 0.2 ml S.B.V. acupuncture treated group than control group. It is concluded that bee venom acupuncture therapy promoted the expression of various cytokines, enzymes and receptors related to the hair growth cycle for hair growth. This findings indicates that bee venom acupuncture therapy is applicable to the treatment of hair loss.

Keywords

References

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