절패모(浙貝母) 에탄올 추출물의 멜라닌 생성 억제 효과

Inhibitory Effect of Fritillaria Verticillata Willd. var. Thunbergii Bak Ethanol Extract on Melanin Biosynthesis

  • 하태광 (상지대학교 한의과대학, 본초학교실) ;
  • 이부균 (세명대학교 본초방제학교실) ;
  • 윤정록 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 문연자 (원광대학교 한의과대학 해부학교실) ;
  • 우원홍 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 박성하 (부산대학교 한의학전문대학원 약물의학부) ;
  • 이장천 (부산대학교 한의학전문대학원 약물의학부)
  • Ha, Tae-Kwang (Department of Herbology, College of Oriental Medicine, Sangji University) ;
  • Lee, Boo-Kyun (Department of Herbology & Prescriptionology, College of Oriental Medicine, Semyung University) ;
  • Yoon, Jeong-Rock (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Mun, Yeun-Ja (Department of Anatomy, College of Oriental Medicine, Wonkwang University) ;
  • Woo, Won-Hong (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Park, Seong-Ha (Division of Pharmacology and Basic Korean Medicine, School of Korean Medicine, Pusan National University) ;
  • Lee, Jang-Cheon (Division of Pharmacology and Basic Korean Medicine, School of Korean Medicine, Pusan National University)
  • 투고 : 2011.04.14
  • 심사 : 2011.06.08
  • 발행 : 2011.06.25

초록

This study was conducted to evaluate the depigmenting properties of ethanol extract from a Fritillaria verticillata Willd. (EFV) in B16F10 cells. Fritillaria verticillata Willd., a perennial herbaceous plant, has been used as a stimulator of mammary gland, expectorant, blood pressure depressant, antitussive agents in Korean herbal medicine. In the present study, we observed that melanin synthesis of B16F10 cells were significantly decreased by EFV without cytotoxicity. However, EFV could not suppress tyrosinase activity in B16F10 cells and mushroom tyrosinase activity. Furthermore, EFV did not effect the protein expression of tyrosinase, tyrosinase-related protein -1 (TRP-1), and TRP-2. These results suggest that EFV inhibited melanin synthesis and the hypopigmentary effect of EVF was not due to regulation of tyrosinase protein.

키워드

참고문헌

  1. 박경아 外. 조직학. 서울, 고려의학, pp 405-411, 1999.
  2. Matsumura, Y. and Ananthaswamy, H.N. Toxic effects of ultraviolet radiation on the skin. Toxicol Appl Pharmacol 195: 298-308, 2004. https://doi.org/10.1016/j.taap.2003.08.019
  3. Chalmers, A.H., Lavin, M., Atisoontornkul, S., Mansbridge, J., Kidson, C. Resistance of human melanoma cells to ultraviolet radiation. Cancer Res. 36: 1930-1934, 1976.
  4. Bissett, D.L., Chatterjee, R., Hannon, D.P. Chronic ultraviolet radiation-induced increase in skin iron and the photoprotective effect of topically applied iron chelators. Photochem Photobio 54: 215-223, 1991. https://doi.org/10.1111/j.1751-1097.1991.tb02009.x
  5. Urabe, K., Aroca, P., Tsukamoto, K., Mascagna, D., Paulumbo, A., Prota, G., Hearing, V.J. The inherent cytotoxicty of melanin precursors. Biochim Biophys Acta 1221: 272-278, 1994. https://doi.org/10.1016/0167-4889(94)90250-X
  6. Kang, S., Cho, S., Chung, J.H., Hammerberg, C., Fisher, G.J., Voorhees, J.J. Inflammation and extracellular matrix degradation mediated by activated transcription factors nuclear factor-kB and activator protein-1 in Inflammatory acne lesions in vivo. Am J Pathol 166: 1691-1699, 2005. https://doi.org/10.1016/S0002-9440(10)62479-0
  7. Paik, J.H., Lee, M.H. Antimelanogenic effects of arbutin. 대한피부과학회지 38(10):1303-1308, 2000.
  8. 이주상, 김정아, 조세훈, 손애량, 장태수, 소명숙, 정시련, 이승호. 감초의 tyrosinase 활성 억제 성분. 생약학회지 34(1):33-39, 2003.
  9. 문연자, 김 진, 임난영, 이승연, 곽 섭, 황충연, 우원홍. 감초물추출물의 멜라닌 형성 억제효과. 동의생리병리학회지 16(6):1230-1235, 2002.
  10. 金昌玟, 辛民敎, 安德均, 李京淳外. 中藥大辭典 서울, 鼎談出版社, pp 3788-3791, 1997.
  11. 韓國生藥學敎授協議會 編著. 本草學, 서울, 아카데미서적, pp 603-605, 2004.
  12. Mosmann, T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65(1-2):55-63, 1983. https://doi.org/10.1016/0022-1759(83)90303-4
  13. Hosoi, J., Abe, E., Suda, T., Kuroki, T. Regulation of melanin synthesis of B16 mouse melanoma cells by 1 alpha, 25-dihydroxyvitamin D3 and retinoic acid. Cancer Res 45(4):1474-1478, 1985.
  14. Martinez-Esparza, M., Jimenez-Cervantes, C., Solano, F., Lozano, J.A., Garcia-Borron, J.C. Mechanisms of melanogenesis inhibition by tumor necrosis factor-alpha in B16/F10 mouse melanoma cells. Eur J Biochem 255(1):139-146, 1998. https://doi.org/10.1046/j.1432-1327.1998.2550139.x
  15. Kitajima, J., Noda, N., Ida, Y., Miyahara, K., Kawasaki, T. Steroid alkaloids of fresh bulbs of Fritillaria thurnbergi Miq. and of crude drug "Bai-Mo" prepared there form. Heterocycles 15: 791-796, 1981. https://doi.org/10.3987/S-1981-02-0791
  16. Qian, B.C., Xu, H.J. Studies on the antitussive and sedative activities of peimine and peiminine. Yao Xue Xue Bao 20(4):306-308, 1985.
  17. Akici, A., Karaalp, A., Skender, E., EI-Fakahany, E.E., Oktay, S. Muscarinic M(2) receptors are not primarily involved in the contraction of guinea-pig gallbladder smooth muscle. Pharmacol Res 40(5):443-449, 1999. https://doi.org/10.1006/phrs.1999.0536
  18. Akbulut, H., Goren, Z., Iskender, E., Eraslsn, A., Ozdemir, O., Oktay, S. Subtypes of muscarinic receptors in rat duodenum: a comparison with rabbit vas deferens, rat atria, guinea-pig ileum and gallbladder by using imperialine. Gen Pharmacol 32(4):505-511, 1993.
  19. Park, Y.J., Ho, Y., Shu, H.S., Shin, J.W., Lee, S.K. Distribution of Medicinal Constituents and content Korean J. Bread. 25: 146-150, 2003.
  20. 천현자, 최은영, 윤성찬, 남항우, 백승화, 우원홍. 백출의 에탄올 추출물에의한 melanin 생성 억제 효과. 약학회지 45(3):269-275, 2001.
  21. 강현성 외 4인. 사삼 물 추출액의 멜라닌 형성 억제 효과. 대한안이비인후피부과학회지 17(1):82-93, 2004.
  22. Mishima, Y., Imokawa, G. Selective aberration and pigment loss in melanosomes of maligment melanoma cells in vitro by glydosylation inhibitors: Premelanosomes as glycoprotein. J Ivest Dermaol 81: 106-114, 1983.
  23. Aroca, P., Solano, F., Salinas, C., Garcia-Borron, J.C., Lozano, J.A. Regulation of final phase of melanogenesis. Eur J Biochem 208: 155-163, 1992. https://doi.org/10.1111/j.1432-1033.1992.tb17169.x
  24. Kobayashi, T., Urabe, K., Winter, A.J., et al. Tyrosinase related protein (TRP-1) functions as a DHICA oxidase activity in melanin biosynthesis. EMBO 13: 5818-5825, 1994.
  25. Ando, H., Wen, Z.M., Kim, H.Y., Valencia, J.C., Costin, G.E., Watabe, H., Yasumoto, K., Niki, Y., Kondoh, H., Ichihashi, M., Hearing, V.J. Intracellular composition of fatty acid affects the processing and function of tyrosinase through the ubiquitin-proteasome pathway. Biochem J 394: 43-50, 2006. https://doi.org/10.1042/BJ20051419
  26. Busca, R., Ballotti, R. Cyclic AMP a key messenger in the regulation of skin pigmentation. Pigment Cell Res 13: 60-69, 2000. https://doi.org/10.1034/j.1600-0749.2000.130203.x
  27. Sassone-Corsi, P. Coupling gene expression to cAMP signalling: role of CREB and CREM. Int J Biochem Cell Biol 30: 27-38, 1998. https://doi.org/10.1016/S1357-2725(97)00093-9
  28. Chen, J.S., Wei, C., Marshall, M.R. Inhibition mechannism of Kojic acid on polyphenol oxidase. J Agric Food Chem 39: 1897-1901, 1991. https://doi.org/10.1021/jf00011a001
  29. Battaini, G., Monzani, E., Casella, L., Santagostini, L., Pagliarin, R. Inhibition of the catecholase activity of biomimetic dinuclear copper complexes by kojic acid. J Biol Inorg Chem 5: 262-268, 2000. https://doi.org/10.1007/s007750050370
  30. Matsuki, M., Watanabe, T., Ogasawara, A., Mikami, T., Matsumoto, T. [Inhibitory mechanism of melanin synthesis by glutathione. Yakugaku Zasshi. 128(8):1203-1237, 2008. https://doi.org/10.1248/yakushi.128.1203